1887

Abstract

cDNA expression vectors of (PLRV) were used to analyse specific mutations in the proteinase and replicase domains of the proteins encoded by ORF1 and ORF2. -mediated DNA transfer was used to introduce a PLRV RNA expression unit, controlled by the 35S promoter of , into potato leaf cells. Expression of unmodified PLRV cDNA led to the replication of viral genomic and subgenomic RNAs and accumulation of the viral capsid protein, whereas alteration of amino acids GDD513–515 of the replicase to VHD abolished PLRV replication. Mutations in the presumed H-D-S catalytic triad of the viral proteinase abolished the formation of viral genomic and subgenomic RNAs as well as synthesis of the viral capsid protein. Co-agroinoculation of the GDD mutant along with any of the proteinase mutants restored virus replication in leaf discs, showing that these mutants are able to complement each other. Moreover, mutation of the postulated serine residue of the catalytic triad of the proteinase altered the pattern of proteins synthesized in comparison to wild-type, further supporting the relevance of the H-D-S motif.

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2001-06-01
2020-05-26
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References

  1. Amberg S. M., Nestorowicz A., McCourt D. W., Rice C. M.. 1994; NS2B-3 proteinase-mediated processing in the yellow fever virus structural region: in vitro and in vivo studies. Journal of Virology68:3794–3802
    [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. 1987; Current Protocols in Molecular Biology New York: Greene Publishing Associates/Wiley-Interscience;
    [Google Scholar]
  3. Babé L. M., Craik Ch. S. 1997; Viral proteases: evolution of diverse structural motifs to optimize function. Cell91:427–430
    [Google Scholar]
  4. Bailey C. J., Lockhart B. P., Redpath M. B., Smith T. P.. 1995; The epidermolytic (exfoliative) toxins of Staphylococcus aureus . Medical Microbiology and Immunology184:53–61
    [Google Scholar]
  5. Bazan J. F., Fletterick R. J.. 1990; Structural and catalytic models of trypsin-like viral proteases. Seminars in Virology1:311–322
    [Google Scholar]
  6. Beck D. L., Forster R. L., Bevan M. W., Boxen K. A., Lowe S. C.. 1990; Infectious transcripts and nucleotide sequence of cloned cDNA of the potexvirus white clover mosaic virus. Virology177:152–158
    [Google Scholar]
  7. Bernstein H. D., Sarnow P., Baltimore D.. 1986; Genetic complementation among poliovirus mutants derived from an infectious cDNA clone. Journal of Virology60:1040–1049
    [Google Scholar]
  8. Bevan M.. 1984; Binary Agrobacterium vectors for plant transformation. Nucleic Acids Research12:8711–8721
    [Google Scholar]
  9. Boyer J.-Ch., Haenni A.-L.. 1994; Infectious transcripts and cDNA clones of RNA viruses. Virology198:415–426
    [Google Scholar]
  10. Charini W. A., Burns C. C., Ehrenfeld E., Semler B. L.. 1991; Trans-rescue of a mutant poliovirus RNA polymerase function. Journal of Virology65:2655–2665
    [Google Scholar]
  11. Daros J. A., Carrington J. C.. 1997; RNA binding activity of NIa proteinase of tobacco etch potyvirus. Virology237:327–336
    [Google Scholar]
  12. De Francesco R., Urbani A., Nardi M. C., Tomei L., Steinkuhler C., Tramontano A.. 1996; A zinc binding site in viral serine proteinases. Biochemistry35:13282–13287
    [Google Scholar]
  13. Demler S. A., Rucker D. G., de Zoeten G. A.. 1993; The chimeric nature of the genome of pea enation mosaic virus: the independent replication of RNA2. Journal of General Virology74:1–14
    [Google Scholar]
  14. Ding J., McGrath W. J., Sweet R. M., Mangel W. F.. 1996; Crystal structure of the human adenovirus proteinase with its 11 amino acid cofactor. EMBO Journal15:1778–1783
    [Google Scholar]
  15. Dore J.-M., Erny C., Pinck L.. 1990; Biologically active transcripts of alfalfa mosaic virus RNA3. FEBS Letters264:183–186
    [Google Scholar]
  16. Dougherty W. G., Semler B. L.. 1993; Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymes. Microbiological Reviews57:781–822
    [Google Scholar]
  17. Eggen R., Verver J., Wellink J., De Jong A., Goldbach R., van Kammen A.. 1989; Improvements of the infectivity of in vitro transcripts from cloned cowpea mosaic virus cDNA: impact of terminal nucleotide sequences. Virology173:447–455
    [Google Scholar]
  18. Elmer J. S., Brand L., Sunter G., Gardiner W. E., Bisaro D. M., Rogers S. G.. 1988; Genetic analysis of the tomato golden mosaic virus. II. The product of the AL1 coding sequence is required for replication. Nucleic Acids Research16:7043–7060
    [Google Scholar]
  19. Frisch D. A., Harris-Haller L. W., Yokubaitis N. T., Thomas T. L., Hardin S. H., Hall T. C.. 1995; Complete sequence of the binary vector Bin19. Plant Molecular Biology27:405–409
    [Google Scholar]
  20. Gorbalenya A. E., Donchenko A. P., Blinov V. M., Koonin E. V.. 1989; Cysteine proteases of positive strand RNA viruses and chymotrypsin-like serine proteases. A distinct protein superfamily with a common structural fold. FEBS Letters243:103–114
    [Google Scholar]
  21. Grimsley N., Hohn T., Davies J. W., Hohn B.. 1987; Agrobacterium -mediated delivery of infectious maize streak virus into maize plants. Nature325:177–179
    [Google Scholar]
  22. Guilley H., Dudley R. K., Jonard G., Balazs E., Richards K. E.. 1982; Transcription of cauliflower mosaic virus DNA: detection of promoter sequences, and characterization of transcripts. Cell30:763–773
    [Google Scholar]
  23. Harrison B. D.. 1984; Potato leafroll virus. CMI/AAB Description of Plant Viruses no 291
    [Google Scholar]
  24. Hong Y., Hunt A. G.. 1996; RNA polymerase activity catalyzed by a potyvirus-encoded RNA-dependent RNA polymerase. Virology226:146–151
    [Google Scholar]
  25. Inokuchi Y., Hirashima A.. 1987; Interference with viral infection by defective RNA replicase. Journal of Virology61:3946–3949
    [Google Scholar]
  26. Jablonski S. A., Morrow C. D.. 1995; Mutation of the aspartic acid residues of the GDD sequence motif of poliovirus RNA-dependent RNA polymerase results in enzymes with altered metal ion requirements for activity. Journal of Virology69:1532–1539
    [Google Scholar]
  27. Joyce C. M., Steitz T. A.. 1994; Function and structure relationships in DNA polymerases. Annual Review of Biochemistry63:777–822
    [Google Scholar]
  28. Kamer G., Argos P.. 1984; Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Research12:7269–7282
    [Google Scholar]
  29. Keese P., Martin R. R., Kawchuk L. M., Waterhouse P. M., Gerlach W. L.. 1990; Nucleotide sequences of an Australian and a Canadian isolate of potato leafroll luteovirus and their relationships with two European isolates. Journal of General Virology71:719–724
    [Google Scholar]
  30. Kim I. C., Kim J. S., Lee S. H., Byun S. M.. 1996; C-terminal peptide of streptokinase, Met369-Pro373, is important in plasminogen activation. Biochemistry and Molecular Biology International40:939–945
    [Google Scholar]
  31. Koonin E. V.. 1991; The phylogeny of RNA-dependent RNA polymerase of positive strand RNA viruses. Journal of General Virology72:2197–2206
    [Google Scholar]
  32. Koonin E. V., Dolja V. V.. 1993; Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Critical Reviews in Biochemistry and Molecular Biology28:375–430
    [Google Scholar]
  33. Kujawa A. B., Drugeon G., Hulanicka D., Haenni A.-L.. 1993; Structural requirements for efficient translational frameshifting in the synthesis of the putative viral RNA-dependent RNA polymerase of potato leafroll virus. Nucleic Acids Research21:2165–2171
    [Google Scholar]
  34. Laemmli U. K.. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature227:680–685
    [Google Scholar]
  35. Leiser R.-M., Ziegler-Graff V., Reutenauer A., Herrbach E., Lemaire O., Guilley H., Richards K., Jonard G.. 1992; Agroinfection as an alternative to insects for infecting plants with beet western yellows luteovirus. Proceedings of the National Academy of Sciences, USA89:9136–9140
    [Google Scholar]
  36. Li X. H., Carrington J. C.. 1995; Complementation of tobacco etch potyvirus mutants by active RNA polymerase expressed in transgenic cells. Proceedings of the National Academy of Sciences, USA92:457–461
    [Google Scholar]
  37. Li X., Ryan M. D., Lamb J. W.. 2000; Potato leafroll virus protein P1 contains a serine proteinase domain. Journal of General Virology81:1857–1864
    [Google Scholar]
  38. Longstaff M., Brigneti G., Boccard F., Chapman S., Baulcombe D.. 1993; Extreme resistance to potato virus X infection in plants expressing a modified component of the putative viral replicase. EMBO Journal12:379–386
    [Google Scholar]
  39. Mattanovich D., Ruker F., da Camara Machado A., Laimer M., Regner F., Steinkellner H., Himmler G., Katinger H.. 1989; Efficient transformation of Agrobacterium spp. by electroporation. Nucleic Acids Research17:6747
    [Google Scholar]
  40. Matthews D. A., Smith W. W., Ferre R. A., Condon B., Budahazi G., Sisson W., Villafranca J. E., Janson C. A., McElroy H. E., Gribskov C. L.. 1994; Structure of human rhinovirus 3C protease reveals a trypsin-like polypeptide fold, RNA-binding site, and means for cleaving precursor polyprotein. Cell77:761–771
    [Google Scholar]
  41. Mayo M. A., Barker H., Robinson D. J., Tamada T., Harrison B. D.. 1982; Evidence that potato leafroll virus RNA is positive-stranded, is linked to a small protein and does not contain polyadenylate. Journal of General Virology59:163–167
    [Google Scholar]
  42. Mayo M. A., Robinson D. J., Jolly C. A., Hyman L.. 1989; Nucleotide sequence of potato leafroll luteovirus RNA. Journal of General Virology70:1037–1051
    [Google Scholar]
  43. Miller W. A., Brown C. M., Wang S.. 1997; New punctuation for the genetic code: luteovirus gene expression. Seminars in Virology8:3–13
    [Google Scholar]
  44. Molinari P., Marusic C., Lucioli A., Tavazza R., Tavazza M.. 1998; Identification of artichoke mottled crinkle virus (AMCV) proteins required for virus replication: complementation of AMCV p33 and p92 replication-defective mutants. Journal of General Virology79:639–647
    [Google Scholar]
  45. Moreno I. M., Malpica J. M., Rodriguez-Cerezo E., Garcia-Arenal F.. 1997; A mutation in tomato aspermy cucumovirus that abolishes cell-to-cell movement is maintained to high levels in the viral RNA population by complementation. Journal of Virology71:9157–9162
    [Google Scholar]
  46. Oster S. K., Wu B., White A. K.. 1998; Uncoupled expression of p33 and p92 permits amplification of tomato bushy stunt virus RNAs. Journal of Virology72:5845–5851
    [Google Scholar]
  47. Pałucha A., Sadowy E., Kujawa A., Juszczuk M., Zagórski W., Hulanicka D.. 1994; Nucleotide sequence of RNA of a Polish isolate of potato leafroll luteovirus. Acta Biochimica Polonica41:405–414
    [Google Scholar]
  48. Petersen J. F., Cherney M. M., Liebig H. D., Skern T., Kuechler E., James M. N.. 1999; The structure of the 2A proteinase from a common cold virus: a proteinase responsible for the shut-off of host-cell protein synthesis. EMBO Journal18:5463–5475
    [Google Scholar]
  49. Pinon J. D., Mayreddy R. R., Turner J. D., Khan F. S., Bonilla P. J., Weiss S. R.. 1997; Efficient autoproteolytic processing of the MHV-A59 3C-like proteinase from the flanking hydrophobic domains requires membranes. Virology230:309–322
    [Google Scholar]
  50. Pringle C. R.. 1998; Virus taxonomy –. San Diego: 1998; Report of the 27th Meeting of the Executive Committee of the International Committee on Taxonomy of Viruses. Archives of Virology143:1449–1459
    [Google Scholar]
  51. Prüfer D., Tacke E., Schmitz J., Kull B., Kaufmann A., Rohde W.. 1992; Ribosomal frameshifting in plants: a novel signal directs the −1 frameshift in the expression of the putative viral replicase of potato leafroll virus. EMBO Journal11:1111–1117
    [Google Scholar]
  52. Prüfer D., Wipf-Schibel C., Richards K., Guiley H., Lecoq H., Jonard G.. 1995; Synthesis of a full-length infectious cDNA clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses. Virology214:150–158
    [Google Scholar]
  53. Prüfer D., Schmitz J., Tacke E., Kull B., Rohde W.. 1997; In vivo expression of a full-length cDNA copy of potato leafroll virus (PLRV) in protoplasts and transgenic plants. Molecular & General Genetics253:609–614
    [Google Scholar]
  54. Prüfer D., Kawchuk L., Monecke M., Nowok S., Fischer R., Rohde W.. 1999; Immunological analysis of potato leafroll luteovirus (PLRV) P1 expression identifies a 25 kDa RNA-binding protein derived via P1 processing. Nucleic Acids Research27:421–425
    [Google Scholar]
  55. Ribas J. C., Wickner R. B.. 1992; RNA-dependent RNA polymerase consensus sequence of the L-A double-stranded RNA virus: definition of essential domains. Proceedings of the National Academy of Sciences, USA89:2185–2189
    [Google Scholar]
  56. Rizzo T. M., Palukaitis P.. 1990; Construction of full-length cDNA clones of cucumber mosaic virus RNA 1, 2 and 3: generation of infectious RNA transcripts. Molecular & General Genetics222:249–256
    [Google Scholar]
  57. Rohde W., Gramstat A., Schmitz J., Tacke E., Prüfer D.. 1994; Plant viruses as model systems for the study of non-canonical mechanisms in higher plants. Journal of General Virology75:2141–2149
    [Google Scholar]
  58. Rubino L., Russo M.. 1998; Membrane targeting sequences in tombusvirus infections. Virology252:431–437
    [Google Scholar]
  59. Rumenapf T., Stark R., Heimann M., Thiel H. J.. 1998; N-terminal protease of pestiviruses: identification of putative catalytic residues by site-directed mutagenesis. Journal of Virology72:2544–2547
    [Google Scholar]
  60. Sadowy E., Pluta K., Gronenborn B., Hulanicka D.. 1998; Infectious transcripts from cloned cDNA of potato leafroll virus. Acta Biochimica Polonica45:611–619
    [Google Scholar]
  61. Sadowy E., Maasen A., Juszczuk M., David C., Zagórski-Ostoja W., Gronenborn B., Hulanicka M. D.. 2001; The ORF0 product of Potato leafroll virus is indispensable for virus accumulation. Journal of General Virology82:1529–1532
    [Google Scholar]
  62. Smith O. P., Harris K. F.. 1990; Potato leafroll virus 3′ genome organization: sequence of the coat protein gene and identification of a viral subgenomic RNA. Phytopathology80:609–614
    [Google Scholar]
  63. Taschner P. E., van der Kuyl A. C., Neeleman L., Bol J. F.. 1991; Replication of an incomplete alfalfa mosaic virus genome in plants transformed with viral replicase genes. Virology181:445–450
    [Google Scholar]
  64. Teterina N. L., Zhou W. D., Cho M. W., Ehrenfeld E.. 1995; Inefficient complementation activity of poliovirus 2C and 3D proteins for rescue of lethal mutations. Journal of Virology69:4245–4254
    [Google Scholar]
  65. Töpfer R., Matzeit V., Gronenborn B., Schell J., Steinbiss H. H.. 1987; A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Research15:5890
    [Google Scholar]
  66. van der Wilk F., Huisman M. J., Cornelissen B. J., Huttinga H., Goldbach R.. 1989; Nucleotide sequence and organization of potato leafroll virus genomic RNA. FEBS Letters245:51–56
    [Google Scholar]
  67. van der Wilk F., Verbeek M., Dullemans A. M., van den Heuvel J. F. J. M.. 1997; The genome-linked protein of potato leafroll virus is located downstream of the putative protease domain of the ORF1 product. Virology234:300–303
    [Google Scholar]
  68. Veidt I., Bouzoubaa S. E., Leiser R.-M., Ziegler-Graff V., Guilley H., Richards K., Jonard G.. 1992; Synthesis of full-length transcripts of beet western yellows virus RNA: messenger properties and biological activity in protoplasts. Virology186:192–200
    [Google Scholar]
  69. Weiland J. J., Dreher T. W.. 1993; Cis-preferential replication of the turnip yellow mosaic virus RNA genome. Proceedings of the National Academy of Sciences, USA90:6095–6099
    [Google Scholar]
  70. White K. A., Skuzeski J. M., Li W., Wei N., Morris T. J.. 1995; Immunodetection, expression strategy and complementation of turnip crinkle virus p28 and p88 replication component. Virology211:525–534
    [Google Scholar]
  71. Wu F. S., Wang M. Y.. 1984; Extraction of proteins for sodium dodecyl sulfate–polyacrylamide gel electrophoresis from protease-rich plant tissues. Analytical Biochemistry139:100–103
    [Google Scholar]
  72. Young M. J., Kelly L., Larkin P. J., Waterhouse P. M., Gerlach W. L.. 1990; Infectious in vitro transcripts from a cloned cDNA of barley yellow dwarf virus. Virology180:372–379
    [Google Scholar]
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