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Journal of General Virology header logo Journal of General Virology

Volume 82, Issue 2

Banana bunchy top nanovirus DNA-1 encodes the ‘master’ replication initiation protein No Access

Abstract

Banana bunchy top nanovirus has a multicomponent, circular single-stranded DNA genome comprising at least six integral components, BBTV DNA-1 to -6, which have been consistently associated with bunchy top disease worldwide. At least three other components, BBTV S1, S2 and Y, which have been isolated from Taiwanese BBTV isolates, do not appear to be integral components. We show here that both BBTV DNA-1 and S1, which encode replication initiation (Rep) proteins, were capable of self-replication when bombarded into banana embryogenic cell suspensions. However, only BBTV DNA-1 was capable of directing the replication of two other BBTV genomic components, namely BBTV DNA-3 which encodes the coat protein, and DNA-5 which encodes a retinoblastoma binding-like protein. These results indicate that (i) BBTV DNA-1 is the minimal replicative unit of BBTV and encodes the ‘master’ viral Rep and (ii) BBTV S1 is possibly a satellite DNA which is unable to replicate integral BBTV components.

  • Received:
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© Microbiology Society
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/content/journal/jgv/10.1099/0022-1317-82-2-459
2001-02-01
2025-04-28
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References

  1. Aronson M. N., Meyer A. D., Gyorgyey J., Katul L., Vetten H. J., Gronenborn B., Timchenko T. 2000; Clink, a nanovirus-encoded protein, binds both pRB and SKP1. Journal of Virology 74:2967–2972
     [Google Scholar]
  2. Beetham P. R., Hafner G. J., Harding R. M., Dale J. L. 1997; Two mRNAs are transcribed from banana bunchy top virus DNA-1. Journal of General Virology 78:229–236
     [Google Scholar]
  3. Beetham P. R., Harding R. M., Dale J. L. 1999; Banana bunchy top virus DNA-2 to 6 are monocistronic. Archives of Virology 144:89–105
     [Google Scholar]
  4. Boevink P., Chu P. W. G., Keese P. 1995; Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses. Virology 207:354–361
     [Google Scholar]
  5. Burns T. M., Harding R. M., Dale J. L. 1995; The genome organization of banana bunchy top virus: analysis of six ssDNA components. Journal of General Virology 76:1471–1482
     [Google Scholar]
  6. Dugdale B., Becker D. K., Harding R. M., Dale J. L. 1998; Promoter activity associated with the intergenic regions of banana bunchy top virus DNA-1 to-6 in transgenic tobacco and banana cells. Journal of General Virology 79:2301–2311
     [Google Scholar]
  7. Hafner G. J., Harding R. M., Dale J. L. 1997a; A DNA primer associated with banana bunchy top virus. Journal of General Virology 78:479–486
     [Google Scholar]
  8. Hafner G. J., Stafford M. R., Wolter L. C., Harding R. M., Dale J. L. 1997b; Nicking and joining activity of banana bunchy top virus replication protein in vitro . Journal of General Virology 78:1795–1799
     [Google Scholar]
  9. Harding R. M., Burns T. M., Hafner G. J., Dietzgen R. G., Dale J. L. 1993; Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase. Journal of General Virology 74:323–328
     [Google Scholar]
  10. Horser C. L., Karan M., Harding R. M., Dale J. L. 2000; Additional Rep-encoding DNAs associated with banana bunchy top virus. Archives of Virology 145:1–6
     [Google Scholar]
  11. Karan M., Harding R. M., Dale J. L. 1994; Evidence for two groups of banana bunchy top virus isolates. Journal of General Virology 75:3541–3546
     [Google Scholar]
  12. Karan M., Harding R. M., Dale J. L. 1997; Association of banana bunchy top virus DNA components 2 to 6 with banana bunchy top disease. Molecular Plant Pathology On-line [http://www.bspp.org.uk/mppol/]
    [Google Scholar]
  13. Katul L., Maiss E., Vetten H. J. 1995; Sequence analysis of a faba bean necrotic yellows virus DNA component containing a putative replicase gene. Journal of General Virology 76:475–479
     [Google Scholar]
  14. Mahon R. E., Bateson M. F., Chamberlain D. A., Higgins C. M., Drew R. A., Dale J. L. 1996; Transformation of an Australian variety of Carica papaya using microprojectile bombardment. Australian Journal of Plant Physiology 23:679–685
     [Google Scholar]
  15. Sano Y., Wada M., Hashimoto Y., Matsumoto T., Kojima M. 1998; Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome. Journal of General Virology 79:3111–3118
     [Google Scholar]
  16. Southern E. 1985; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
     [Google Scholar]
  17. Stewart C. N., Via L. E. 1993; A rapid CTAB DNA isolation technique for RAPD fingerprint and PCR applications. BioTechniques 14:748–750
     [Google Scholar]
  18. Timchenko T., Kouchkovsky F., Katul L., David C., Vetten H. J., Gronenborn B. 1999; A single Rep protein initiates replication of multiple genome components of faba bean necrotic yellows virus, a single-stranded DNA virus of plants. Journal of Virology 73:10173–10182
     [Google Scholar]
  19. Timchenko T., Katul L., Sano Y., Kouchkovsky F., Vetten H. J., Gronenborn B. 2000; The master Rep concept in nanovirus replication: identification of missing genome components and potential for natural genetic reassortment. Virology 274:189–195
     [Google Scholar]
  20. Wanitchakorn R., Harding R. M., Dale J. L. 1997; Banana bunchy top virus DNA-3 encodes the viral coat protein. Archives of Virology 142:1673–1680
     [Google Scholar]
  21. Wanitchakorn R., Hafner G. J., Harding R. M., Dale J. L. 2000a; Functional analysis of proteins encoded by banana bunchy top virus DNA-4 to -6. Journal of General Virology 81:299–306
     [Google Scholar]
  22. Wanitchakorn R., Harding R. M., Dale J. L. 2000b; Sequence variability in the coat protein gene of two groups of banana bunchy top isolates. Archives of Virology 145:1–10
     [Google Scholar]
  23. Wu R. Y., You L. R., Soong T. S. 1994; Nucleotide sequence of two circular single-stranded DNA‘s associated with banana bunchy top virus. Phytopathology 84:952–958
     [Google Scholar]
  24. Yeh H., Su H. J., Chao Y. 1994; Genome characterization and identification of viral-associated dsDNA component of banana bunchy top virus. Virology 198:645–652
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-82-2-459
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Banana bunchy top nanovirus DNA-1 encodes the ‘master’ replication initiation protein
J. Gen. Virol. 82, 459 (2001); https://doi.org/10.1099/0022-1317-82-2-459
/content/journal/jgv/10.1099/0022-1317-82-2-459
/content/journal/jgv/10.1099/0022-1317-82-2-459
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