1887

Abstract

To determine an antiapoptotic activity of poliovirus type 1 (PV-1), we examined the effect of PV-1 infection on apoptosis that was induced in HEp-2 cells by the treatment with 1 M sorbitol. The virus did not induce apoptosis in the infected cells and could suppress both the fragmentation of chromosomal DNA and morphological cell and cell nuclei changes in the sorbitol-treated cells, indicating that PV-1 induces an antiapoptotic state. Comparison of the kinetics showed that this ability of the virus appeared in the infected cells at the time of progeny virus formation (maturation step of virus multiplication). Simultaneously with this antiapoptotic activity, PV-1 infection also suppressed non-apoptotic cell death induced by sodium chloride. Electron microscopic observation revealed that the cells killed by the sodium chloride treatment had undergone liquefactive necrosis, indicating that PV-1 can inhibit both apoptosis and necrosis. In addition, PV-1 can grow in the apoptotic cells, although the virus yield was reduced to a quarter of the yield in normal cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-12-2965
2001-12-01
2020-09-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/12/0822965a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-12-2965&mimeType=html&fmt=ahah

References

  1. Agol V. I., Belov G. A., Bienz K., Egger D., Kolesnikova M. S., Romanova L. I., Sladkova L. V., Tolskaya E. A.. 2000; Competing death programs in poliovirus-infected cells: commitment switch in the middle of the infectious cycle. Journal of Virology74:5534–5541
    [Google Scholar]
  2. Ammendolia M. G., Tinari A., Calcabrini A., Superti F.. 1999; Poliovirus infection induces apoptosis in CaCo-2 cells. Journal of Medical Virology59:122–129
    [Google Scholar]
  3. Barco A., Feduchi E., Carrasco L.. 2000; Poliovirus protease 3C(pro) kills cells by apoptosis. Virology266:352–360
    [Google Scholar]
  4. Fukumori T., Akari H., Iida S., Hata S., Kagawa S., Aida Y., Koyama A. H., Adachi A.. 1998; The HIV-1 Vpr displays strong anti-apoptotic activity. FEBS Letters432:17–20
    [Google Scholar]
  5. Girard S., Couderc T., Destombes J., Thiesson D., Delpeyroux F., Blondel B.. 1999; Poliovirus induces apoptosis in the mouse central nervous system. Journal of Virology73:6066–6072
    [Google Scholar]
  6. Goldstaub D., Gradi A., Bercovitch Z., Grosmann Z., Nophar Y., Luria S., Sonenberg N., Kahana C.. 2000; Poliovirus 2A protease induces apoptotic cell death. Molecular and Cellular Biology20:1271–1277
    [Google Scholar]
  7. Goudie R. B.. 1985; Molecular and cellular pathology of tissue damage. In Muir’s Textbook of Pathology , 12th edn. Edited by Anderson J. R.. Baltimore, MD: Edward Arnold;
    [Google Scholar]
  8. Hata S., Koyama A. H., Shiota H., Adachi A., Goshima F., Nishiyama Y.. 1999; Antiapoptotic activity of herpes simplex virus type 2: a role of US3 protein kinase gene. Microbes and Infection1:601–607
    [Google Scholar]
  9. Hirt B.. 1967; Selective extraction of polyoma DNA from infected mouse cell culture. Journal of Molecular Biology26:365–369
    [Google Scholar]
  10. Kerr J. F. R., Harmon B. V.. 1991; Definition and incidence of apoptosis: an historical perspective In Apoptosis: The Molecular Basis of Cell Death. pp5–29 Edited by Tomei L. D., Cope F. O. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  11. Koyama A. H.. 1995; Induction of apoptotic DNA fragmentation by the infection of vesicular stomatitis virus. Virus Research37:285–290
    [Google Scholar]
  12. Koyama A. H., Adachi A.. 1997; Induction of apoptosis by herpes simplex virus type 1. Journal of General Virology78:2909–2912
    [Google Scholar]
  13. Koyama A. H., Miwa Y.. 1997; Suppression of apoptotic DNA fragmentation in herpes simplex virus type 1-infected cells. Journal of Virology71:2567–2571
    [Google Scholar]
  14. Koyama A. H., Uchida T.. 1994; Inhibition by brefeldin A of the envelopment of nucleocapsids in herpes simplex virus type 1-infected Vero cells. Archives of Virology135:305–317
    [Google Scholar]
  15. Koyama A. H., Irie H., Fukumori T., Hata S., Iida S., Akari H., Adachi A.. 1998a; Role of virus-induced apoptosis in a host defense mechanism against virus infection. Journal of Medical Investigation45:37–45
    [Google Scholar]
  16. Koyama A. H., Akari H., Adachi A., Goshima F., Nishiyama Y.. 1998b; Induction of apoptosis in HEp-2 cells by infection with herpes simplex virus type 2. Archives of Virology143:2435–2441
    [Google Scholar]
  17. Koyama A. H., Arakawa T., Adachi A.. 1998c; Acceleration of virus-induced apoptosis by tumor necrosis factor. FEBS Letters426:179–182
    [Google Scholar]
  18. Koyama A. H., Arakawa T., Adachi A.. 2000a; Characterization of apoptosis induced by sorbitol: a unique system for the detection of antiapoptotic activities of viruses. Microbes and Infection2:599–606
    [Google Scholar]
  19. Koyama A. H., Fukumori T., Fujita M., Irie H., Adachi A.. 2000b; Physiological significance of apoptosis in animal virus infection. Microbes and Infection2:1111–1117
    [Google Scholar]
  20. Koyama A. H., Ogawa M., Kato A., Nagai Y., Adachi A.. 2001; Lack of apoptosis in the Sendai virus-infected HEp-2 cells without participation of viral antiapoptosis gene. Microbes and Infection 3 (in press
    [Google Scholar]
  21. Kurokawa M., Koyama A. H., Yasuoka S., Adachi A.. 1999; Influenza virus overcomes apoptosis by rapid multiplication. International Journal of Molecular Medicine3:527–530
    [Google Scholar]
  22. Lopez-Guerrero J. A., Alonso M., Martin-Belmonte F., Carrasco L.. 2000; Poliovirus induces apoptosis in the human U937 promonocytic cell line. Virology272:250–256
    [Google Scholar]
  23. McGarrity G. J.. 1979; Detection of contamination. Methods in Enzymology58:18–29
    [Google Scholar]
  24. Roulston A., Marcellus R. C., Branton P. E.. 1999; Viruses and apoptosis. Annual Review of Microbiology53:577–628
    [Google Scholar]
  25. Shen Y., Shenk T. E.. 1995; Viruses and apoptosis. Current Opinion in Genetics & Development5:105–111
    [Google Scholar]
  26. Tolskaya E. A., Romanova L. I., Kolesnikova M. S., Ivannikova T. A., Smirnova E. A., Raikhlin N. T., Agol V. I.. 1995; Apoptosis-inducing and apoptosis-preventing functions of poliovirus. Journal of Virology69:1181–1189
    [Google Scholar]
  27. Yasuda H., Toida S.. 1986; Application of pop-off method to bone marrow and peripheral blood specimens for purposes of electron microscopy. Ultrastructural Pathology10:577–582
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-12-2965
Loading
/content/journal/jgv/10.1099/0022-1317-82-12-2965
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error