1887

Abstract

Genetic analysis of selected genome regions of hepatitis A virus (HAV) suggested that distinct genotypes of HAV could be found in different geographical regions. In order to gain insight into the genetic variability and mode of evolution of HAV in South America, an analysis was performed of sequence data obtained from the VP1 amino terminus and the VP1/2A region of HAV strains isolated over a short period of time in Uruguay, Argentina and Chile. Sequences obtained from 22 distinct HAV isolates were compared with published sequences from 21 different strains isolated all over the world. Phylogenetic analysis revealed that all strains isolated belong to a unique sub-genotype (IA). Strains isolated during an outbreak period showed a higher degree of heterogeneity than anticipated previously and the co-circulation of different isolates. The genetic variability among strains isolated in this region seems to be higher in comparison with strains isolated in other regions of the world.

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2001-11-01
2020-06-05
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References

  1. Apaire-Marchais V., Robertson B. H., Aubineau-Ferre V., Le Roux M. G., Leveque F., Schwartzbrod L., Billaudel S.. 1995; Direct sequencing of hepatitis A virus strains isolated during an epidemic in France. Applied and Environmental Microbiology61:3977–3980
    [Google Scholar]
  2. Bruisten S. M., Van Steenbergen J. E., Pijl A. S., Niesters H. G. M., Van Doornum G. J. J., Coutinho R. A.. 2001; Molecular epidemiology of hepatitis A virus in Amsterdam, the Netherlands. Journal of Medical Virology63:88–95
    [Google Scholar]
  3. Cohen J. I., Ticehurst J. R., Purcell R. H., Buckler-White A., Baroudy B. M.. 1987; Complete nucleotide sequence of wild-type hepatitis A virus: comparison with different strains of hepatitis A virus and other picornaviruses. Journal of Virology61:50–59
    [Google Scholar]
  4. Felsenstein J.. 1993; Phylogeny inference package, version 3.5 Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  5. Jukes T. H., Cantor C. R.. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp121–132 Edited by Munro H. N.. New York: Academic Press;
    [Google Scholar]
  6. Kedda M. A., Kew M. C., Cohn R. J., Field S. P., Schwyzer R., Song E., Fernandes-Costa F.. 1995; An outbreak of hepatitis A among South African patients with haemophilia: evidence implicating contaminated factor VIII concentrate as the source. Hepatology22:1363–1367
    [Google Scholar]
  7. Khanna B., Spelbring J. E., Innis B. L., Robertson B. H.. 1992; Characterization of a genetic variant of human hepatitis A virus. Journal of Medical Virology36:118–124
    [Google Scholar]
  8. Kumar S., Tamura K., Nei M.. 1994; MEGA: molecular evolutionary genetic analysis software for microcomputers. Computer Applications in the Biosciences10:189–191
    [Google Scholar]
  9. Matthews R. E. F.. editor 1982; Classification and nomenclature of viruses. Fourth Report of the International Committee on Taxonomy of Viruses. Intervirology171–199
    [Google Scholar]
  10. Melnick J. L.. 1982; Classification of hepatitis A virus as enterovirus type 72 and of hepatitis B virus as hepadnavirus type 1. Intervirology18:105–106
    [Google Scholar]
  11. Normann A., Pfisterer-Hunt M., Schade S., Graff J., Chaves R. L., Crovari P., Icardi G., Flehmig B.. 1995; Molecular epidemiology of an outbreak of hepatitis A in Italy. Journal of Medical Virology47:467–471
    [Google Scholar]
  12. Palmenberg A. C.. 1989; Sequence alignments of picornaviral capsid proteins. In Molecular Aspects of Picornaviral Infection and Detection. Edited by Semler B. L., Ehenfeld E.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  13. Robertson B. H., Nainan O. V.. 1997; Genetic and antigenic variants of hepatitis A virus. In Viral Hepatitis and Liver Diseases pp14–18 Edited by Rizzetto M., Purcell R. H., Verme G.. Torino: Edizioni Minerva Medica;
    [Google Scholar]
  14. Robertson B. H., Brown V. K., Khanna B.. 1989; Altered hepatitis A VP1 protein resulting from cell culture propagation of virus. Virus Research13:207–212
    [Google Scholar]
  15. Robertson B. H., Khanna B., Nainan O. V., Margolis H. S.. 1991; Epidemiologic patterns of wild-type hepatitis A virus determined by genetic variation. Journal of Infectious Diseases163:286–292
    [Google Scholar]
  16. Robertson B. H., Jansen R. W., Khanna B., Totsuka A., Nainan O. V., Siegl G., Widell A., Margolis H. S., Isomura S., Ito K., Ishizu T., Moritsugu Y., Lemon S. W.. 1992; Genetic relatedness of hepatitis A virus strains recovered from different geographical regions. Journal of General Virology73:1365–1377
    [Google Scholar]
  17. Robertson B. H., Averhoff F., Cromeans T. L., Han X., Khoprasert B., Nainan O. V., Rosenberg J., Paikoff L., DeBess E., Shapiro C. N., Margolis H. S.. 2000; Genetic relatedness of hepatitis A virus isolates during a community-wide outbreak. Journal of Medical Virology62:144–150
    [Google Scholar]
  18. Tamura K., Nei M.. 1993; Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution10:512–526
    [Google Scholar]
  19. Tanaka J.. 2000; Hepatitis A shifting epidemiology in Latin America. Vaccine18:Suppl. 1S57–S60
    [Google Scholar]
  20. Tapia-Conyer R., Santos J. I., Cavalcanti A. M., Urdaneta E., Rivera L., Manterola A., Potin M., Ruttiman R., Tanaka Kido J.. 1999; Hepatitis A in Latin America: a changing epidemiologic pattern. American Journal of Tropical Medicine and Hygiene61:825–829
    [Google Scholar]
  21. Taylor M. B.. 1997; Molecular epidemiology of South African strains of hepatitis A virus: 1982–1996. Journal of Medical Virology51:273–279
    [Google Scholar]
  22. Thompson J. D., Higgins D. G., Gibson T. J.. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research22:4673–4680
    [Google Scholar]
  23. Ticehurst J., Cohen J. I., Purcell R. H.. 1988; Analysis of molecular sequences demonstrates that hepatitis A virus (HAV) is a unique picornavirus. In Viral Hepatitis and Liver Disease pp33–35 Edited by Zuckerman A. J.. New York: Alan R. Liss;
    [Google Scholar]
  24. Ticehurst J., Cohen J. I., Feinstone S. M., Purcell R. H., Jansen R. W., Lemon S. M.. 1989; Replication of hepatitis A virus: new ideas from studies with cloned cDNA. In Molecular Aspects of Picornaviral Infection and Detection pp27–50 Edited by Semler B. L., Ehenfeld E.. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  25. Wimmer E., Murdin A.. 1991; Hepatitis A and the molecular biology of picornaviruses: a case for a new genus of the family Picornaviridae . In Viral Hepatitis and Liver Disease pp1–41 Edited by Hollinger F. B., Margolis H. S., Lemon S. M.. Baltimore: Williams & Wilkins;
    [Google Scholar]
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