1887

Abstract

RNAs 1 and 2 of the tripartite genome of (AMV) encode the two viral replicase subunits. Full-length DNA copies of RNAs 1 and 2 were used to transform tobacco plants (R12 lines). None of the transgenic lines showed resistance to AMV infection. In healthy R12 plants, the transcripts of the viral cDNAs were copied by the transgenic viral replicase into minus-strand RNAs but subsequent steps in replication were blocked. When the R12 plants were inoculated with AMV RNA 3, this block was lifted and the transgenic RNAs 1 and 2 were amplified by the transgenic replicase together with RNA 3. The transgenic expression of RNAs 1 and 2 largely circumvented the role of coat protein (CP) in the inoculum that is required for infection of nontransgenic plants. The results for the first time demonstrate the role of CP in AMV plus-strand RNA synthesis at the whole plant level.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-82-1-25
2001-01-01
2024-05-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/82/1/0820025a.html?itemId=/content/journal/jgv/10.1099/0022-1317-82-1-25&mimeType=html&fmt=ahah

References

  1. Angell S. A., Baulcombe D. C. 1997; Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA. EMBO Journal 16:3675–3684
    [Google Scholar]
  2. Bol J. F., van Vloten-Doting L., Jaspars E. M. J. 1971; A functional equivalence of top component a RNA and coat protein in the initiation of infection by alfalfa mosaic virus. Virology 46:73–85
    [Google Scholar]
  3. de Graaff M., Man in’t Veld M. R., Jaspars E. M. J. 1995; In vitro evidence that the coat protein of alfalfa mosaic virus plays a direct role in the regulation of plus and minus-RNA synthesis: implications for the life cycle of alfalfa mosaic virus. Virology 208:583–589
    [Google Scholar]
  4. Dever T. E. 1999; Translation initiation: adept at adapting. Trends in Biochemical Sciences 24:398–403
    [Google Scholar]
  5. Gallie D. R. 1991; The cap and poly(A) tail function synergistically to regulate mRNA translational efficiency. Genes & Development 5:2108–2116
    [Google Scholar]
  6. Horsch R. B., Fry J. E., Hoffmann N. L., Eichholtz D., Rogers S. G., Fraley R. T. 1985; A simple and general method for transferring genes into plants. Science 227:1229–1231
    [Google Scholar]
  7. Houser-Scott F., Ansel-McKinney P., Cai J.-M., Gehrke L. 1997; In vitro genetic selection analysis of alfalfa mosaic virus coat protein binding to 3’-terminal AUGC repeats in viral RNAs. Journal of Virology 71:2310–2319
    [Google Scholar]
  8. Houwing C. J., Jaspars E. M. J. 1993; Coat protein stimulates replication complexes of alfalfa mosaic virus to produce virion RNAs in vitro . Biochimie 75:617–622
    [Google Scholar]
  9. Imataka H., Gradi A., Sonenberg N. 1998; A newly identified N-terminal amino acid sequence of human eIF4G binds poly(A) binding protein and functions in poly(A)-dependent translation. EMBO Journal 17:7480–7489
    [Google Scholar]
  10. Iyer L. M., Hall T. C. 2000; Virus recovery is induced in Brome mosaic virus p2 transgenic plants showing synchronous complementation and RNA-2-specific silencing. Molecular Plant–Microbe Interactions 13:247–258
    [Google Scholar]
  11. Kaido M., Mori M., Mise K., Okuno T., Furusawa I. 1995; Inhibition of brome mosaic virus (BMV) amplification in protoplasts from transgenic tobacco plants expressing replicable BMV RNAs. Journal of General Virology 76:2827–2833
    [Google Scholar]
  12. Mori M., Mise K., Okuno T., Furusawa I. 1992; Expression of brome-mosaic virus-encoded replicase genes in transgenic tobacco plants. Journal of General Virology 73:169–172
    [Google Scholar]
  13. Neeleman L., Bol J. F. 1999; Cis -acting functions of alfalfa mosaic virus proteins involved in replication and encapsidation of viral RNA. Virology 254:324–333
    [Google Scholar]
  14. Neeleman L., van der Kuyl A. C., Bol J. F. 1991; Role of alfalfa mosaic virus coat protein gene in symptom formation. Virology 181:687–693
    [Google Scholar]
  15. Neeleman L., van der Vossen E. A. G., Bol J. F. 1993; Infection of tobacco with alfalfa mosaic virus cDNAs sheds light on the early function of the coat protein. Virology 196:883–887
    [Google Scholar]
  16. Olsthoorn R. C. L., Mertens S., Brederode F. Th., Bol J. F. 1999; A conformational switch at the 3’ end of a plant virus RNA regulates viral replication. EMBO Journal 18:4856–4864
    [Google Scholar]
  17. Pacha R. F., Allison R. F., Ahlquist P. 1990; Cis -acting sequences required for in vivo amplification of genomic RNA 3 are organized differently in related bromoviruses. Virology 174:436–443
    [Google Scholar]
  18. Peach C., Velten J. 1991; Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Molecular Biology 17:49–60
    [Google Scholar]
  19. Quadt R., Rosdorff H. J. M., Hunt T. W., Jaspars E. M. J. 1991; Analysis of the protein composition of alfalfa mosaic virus RNA-dependent RNA polymerase. Virology 182:309–315
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  21. Suzuki M., Masuata C., Takanami Y., Kuwata S. 1996; Resistance against cucumber mosaic virus in plants expressing the viral replicon. FEBS Letters 379:26–30
    [Google Scholar]
  22. Taschner P. E. M., van der Kuyl A. C., Neeleman L., Bol J. F. 1991; Replication of an incomplete alfalfa mosaic virus genome in plants transformed with viral replicase genes. Virology 181:445–450
    [Google Scholar]
  23. van der Kuyl A. C., Neeleman L., Bol J. F. 1991a; Complementation and recombination between alfalfa mosaic virus RNA 3 mutants in tobacco plants. Virology 183:731–738
    [Google Scholar]
  24. van der Kuyl A. C., Neeleman L., Bol J. F. 1991b; Role of alfalfa mosaic virus coat protein in regulation of the balance between viral plus and minus strand RNA synthesis. Virology 185:496–499
    [Google Scholar]
  25. van der Vossen E. A. G., Neeleman L., Bol J. F. 1994; Early and late functions of alfalfa mosaic virus coat protein can be mutated separately. Virology 202:891–903
    [Google Scholar]
  26. van Vloten-Doting L., Jaspars E. M. J. 1972; The uncoating of alfalfa mosaic virus by its own RNA. Virology 48:699–708
    [Google Scholar]
  27. Vende P., Piron M., Castagné N., Poncet D. 2000; Efficient translation of rotavirus mRNA requires simultaneous interaction of NSP3 with the eukaryotic translation initiation factor eIF4G and the mRNA 3) end. Journal of Virology 74:7064–7071
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-82-1-25
Loading
/content/journal/jgv/10.1099/0022-1317-82-1-25
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error