1887

Abstract

Six genotypes of hepatitis B virus (HBV) have been described. However, relatively few complete genomes originating from East Asia, where most of the world’s HBV carriers live, have been studied. We analysed five complete HBV genomes of Vietnamese origin, which in our previous studies had produced atypical genotyping patterns. All five strains had HBsAg sequences with markers for serotype . In phylogenetic tree analysis, two of the genomes clustered with genotype C, and three clustered on a separate branch between genotypes A, B and C, suggesting a new genotype. However, these three strains showed signs of recombination in similarity plot and bootscanning analysis. Phylogenetic tree analysis of two segments separately supported recombination between genotype C and a putative new genotype (or possibly a subgroup of genotype A). The segment between nt 1801 and 2865 was clearly of genotype C origin, while the major part of the genome (nt 2866–1800) was placed on a branch close to genotype A. The findings encourage further study of genotypes and recombination in HBV from this geographical region.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-9-2267
2000-09-01
2020-11-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/9/0812267a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-9-2267&mimeType=html&fmt=ahah

References

  1. Bollyky P. L., Rambaut A., Harvey P. H., Holmes E. C.. 1996; Recombination between sequences of hepatitis B virus from different genotypes. Journal of Molecular Evolution42:97–102
    [Google Scholar]
  2. Bowyer S. M., Sim J. G. M.. 2000; Relationships within and between genotypes of hepatitis B virus at points across the genome: footprints of recombination in certain isolates. Journal of General Virology81:379–392
    [Google Scholar]
  3. Felsenstein J.. 1989; PHYLIP: phylogeny inference package (version 3.2. Cladistics5:164–166
    [Google Scholar]
  4. Georgi-Geisberger P., Berns H., Loncarevic I. F., Yu Z. Y., Tang Z. Y., Zentgraf H., Schroder C. H.. 1992; Mutations on free and integrated hepatitis B virus DNA in a hepatocellular carcinoma: footprints of homologous recombination. Oncology49:386–395
    [Google Scholar]
  5. Gerner P. R., Friedt M., Oettinger R., Lausch E., Wirth S.. 1998; The hepatitis B virus seroconversion to anti-HBe is frequently associated with HBV genotype changes and selection of preS2-defective particles in chronically infected children. Virology245:163–172
    [Google Scholar]
  6. Hannoun C., Horal P., Lindh M.. 1999; Long-term mutation rates in the hepatitis B virus genome. Journal of General Virology81:75–83
    [Google Scholar]
  7. Kishino H., Hasegawa M.. 1989; Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. Journal of Molecular Evolution29:170–179
    [Google Scholar]
  8. Li J. S., Tong S. P., Wen Y. M., Vitvitski L., Zhang Q., Trepo C.. 1993; Hepatitis B virus genotype A rarely circulates as an HBe-minus mutant: possible contribution of a single nucleotide in the precore region. Journal of Virology67:5402–5410
    [Google Scholar]
  9. Lindh M., Andersson A. S., Gusdal A.. 1997; Genotypes, nt 1858 variants, and geographic origin of hepatitis B virus – large-scale analysis using a new genotyping method. Journal of Infectious Diseases175:1285–1293
    [Google Scholar]
  10. Lindh M., Gonzalez J. E., Norkrans G., Horal P.. 1998; Genotyping of hepatitis B virus by restriction pattern analysis of a pre-S amplicon. Journal of Virology Methods72:163–174
    [Google Scholar]
  11. Lole K. S., Bollinger R. C., Paranjape R. S., Gadkari D., Kulkarni S. S., Novak N. G., Ingersoll R., Sheppard H. W., Ray S. C.. 1999; Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. Journal of Virology73:152–160
    [Google Scholar]
  12. Norder H., Hammas B., Löfdahl S., Couroucé A. M., Magnius L. O.. 1992; Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains. Journal of General Virology73:1201–1208
    [Google Scholar]
  13. Norder H., Hammas B., Lee S.-D., Bile K., Couroucé A.-M., Mushahwar I. K., Magnius L. O.. 1993; Genetic relatedness of hepatitis B viral strains of diverse geographical origin and natural variations in the primary structure of the surface antigen. Journal of General Virology74:1341–1348
    [Google Scholar]
  14. Norder H., Couroucé A.-M., Magnius L. O.. 1994; Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology198:489–503
    [Google Scholar]
  15. Norder H., Ebert J. W., Fields H. A., Mushahwar I. K., Magnius L. O.. 1996; Complete sequencing of a gibbon hepatitis B virus genome reveals a unique genotype distantly related to the chimpanzee hepatitis B virus. Virology218:214–223
    [Google Scholar]
  16. Okamoto H., Tsuda F., Sakugawa H., Sastrosoewignjo R. I., Imai M., Miyakawa Y., Mayumi M.. 1988; Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. Journal of General Virology69:2575–2583
    [Google Scholar]
  17. Salminen M. O., Carr J. K., Burke D. S., McCutchan F. E.. 1995; Identification of breakpoints in intergenotypic recombinants of HIV type 1 by bootscanning. AIDS Research and Human Retroviruses11:1423–1425
    [Google Scholar]
  18. Strimmer K., Haeseler A.. 1996; Quartet puzzling: a quartet maximum-likelihood method for reconstructing tree topologies. Molecular Biology and Evolution13:964–969
    [Google Scholar]
  19. Stuyver L., De Gendt S., Van Geyt C., Zoulim F., Fried M., Schinazi R. F., Rossau R.. 2000; A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. Journal of General Virology81:67–74
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-9-2267
Loading
/content/journal/jgv/10.1099/0022-1317-81-9-2267
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error