1887

Abstract

The specific-pathogen-free (SPF) flocks of chickens maintained by the Department of Microbiology and Immunology at Cornell University became infected, inadvertently, with chicken anaemia virus (CAV), as demonstrated by seroconversion. Chickens from five flocks representing three different strains were examined for the presence of CAV using nested PCR. Virus was detected in ovaries, infundibula, vas deferentia, testes and spleens. Ovaries were positive in 38 to 72% of the hens in four flocks with 13 to 56 birds examined per flock. Interestingly, the ovaries were often the only positive tissues, while a few hens had only positive spleens. In roosters, the vas deferens was positive in 30 to 79% of the birds with 5 to 19 birds examined per flock; the vas deferens was the only positive tissue in 20 to 37%. Individual cells in the theca externa and rare epithelial cells in the infundibular epithelium were positive for CAV by PCR. Positive cells were not detected in testes or vas deferentia. The SH-1 strain of CAV was isolated from these tissues and partially sequenced. Only minor sequence differences were found compared to CIA-1 and Cux-1. Embryos from matings between persistently infected dams and sire had CAV-positive cells in mesenchyme near the developing vertebral column. The data show that CAV persists in the reproductive tissues far longer than previously thought, and that it can be vertically transmitted from persistently infected birds.

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2000-08-01
2020-04-02
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References

  1. Adair B. M., McNeilly F., McConnell C. D., McNulty M. S.. 1993; Characterization of surface markers present on cells infected by chicken anemia virus in experimentally infected chickens. Avian Diseases37:943–950
    [Google Scholar]
  2. Bassami M. R., Berryman D., Wilcox G. E., Raidal S. R.. 1998; Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anemia virus. Virology249:453–459
    [Google Scholar]
  3. Calnek B. W., Lucio-Martinez B., Cardona C., Harris R. W., Schat K. A., Buscaglia C.. 2000; Comparative susceptibility of Marek’s disease cell lines to chicken infectious anemia virus. Avian Diseases44:114–124
    [Google Scholar]
  4. Cardona C. J., Lucio B., O’Connell P., Jagne J., Schat K. A.. 2000; Humoral immune responses to chicken infectious anemia virus in three strains of chickens in a closed flock. Avian Diseases44: (in press)
    [Google Scholar]
  5. Chandratilleke D., O’Connell P., Schat K. A.. 1991; Characterization of proteins of chicken infectious anemia virus with monoclonal antibodies. Avian Diseases35:854–862
    [Google Scholar]
  6. Chettle N. J., Eddy R. K., Wyeth P. J., Lister S. A.. 1989; An outbreak of disease due to chicken anemia agent in broiler chickens in England. Veterinary Record124:211–215
    [Google Scholar]
  7. Engström B. E., Luthman M.. 1984; Blue wing disease of chickens: experimental infection with a Swedish isolate of chicken anaemia agent and an avian reovirus. Avian Pathology17:23–32
    [Google Scholar]
  8. Etches R. J.. 1996; The male. In Reproduction in Poultry pp208–233 Wallingford, UK: CAB International;
    [Google Scholar]
  9. Fares F., Habib M., Verniol C., Drouet E., Niveleau A.. 1998; In situ amplification of the Epstein–Barr virus genome in cell suspensions. Journal of Virological Methods71:211–218
    [Google Scholar]
  10. Goryo M., Sugimura H., Matsumoto S., Umemura T., Itakura C.. 1985; Isolation of an agent inducing chicken anaemia. Avian Pathology14:483–496
    [Google Scholar]
  11. Goryo M., Shibata Y., Suwa T., Umemura T., Itakura C.. 1987; Outbreak of anemia associated with chicken anemia agent in young chicks. Japanese Journal of Veterinary Science49:867–873
    [Google Scholar]
  12. Goryo M., Suwa T., Umemura T., Itakura C., Yamashiro S.. 1989; Histopathology of chicks inoculated with chicken anaemia agent (MSB1-TK5803 strain. Avian Pathology18:73–89
    [Google Scholar]
  13. Hodges R. D.. 1974; The Histology of the Fowl p– 648 New York: Academic Press;
    [Google Scholar]
  14. Hoop R. K.. 1992; Persistence and vertical transmission of chicken anaemia agent in experimentally infected laying hens. Avian Pathology21:493–501
    [Google Scholar]
  15. Hoop R. K.. 1993; Transmission of chicken anaemia virus with semen. Veterinary Record133:551–552
    [Google Scholar]
  16. Hoop R. K., Reece R. L.. 1991; The use of immunofluorescence and immunoperoxidase staining in studying the pathogenesis of chicken anemia agent in experimentally infected chickens. Avian Pathology20:349–356
    [Google Scholar]
  17. Hoop R. K., Guscetti F., Keller B.. 1992; An outbreak of infectious chicken anemia in fattening chickens in Switzerland. Schweizer Archiv für Tierheilkunde134:485–489
    [Google Scholar]
  18. Hu L.-B., Lucio B., Schat K. A.. 1993a; Abrogation of age-related resistance to chicken infectious anemia by embryonal bursectomy. Avian Diseases37:157–169
    [Google Scholar]
  19. Hu L.-B., Lucio B., Schat K. A.. 1993b; Depletion of CD4+ and CD8+ T lymphocyte subpopulations by CIA-1, a chicken infectious anemia virus. Avian Diseases37:492–500
    [Google Scholar]
  20. Lucio B., Schat K. A., Shivaprasad H. L.. 1990; Identification of the chicken anemia agent, reproduction of the disease, and serological survey in the United States. Avian Diseases34:146–153
    [Google Scholar]
  21. McNulty M. S.. 1991; Chicken anemia agent: a review. Avian Pathology20:187–203
    [Google Scholar]
  22. Miyata H., Tsunoda H., Kazi A., Yamada A., Khan M. A., Murakami J., Kamahora T., Shiraki K., Hino S.. 1999; Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus. Journal of Virology73:3582–3586
    [Google Scholar]
  23. Moore D. D.. 1988; Preparation and analysis of DNA. In Current Protocols in Molecular Biology vol 1 Sections 2.1.1–2.1.10 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K.. New York: Greene Publishing Associates/Wiley Interscience;
    [Google Scholar]
  24. Niagro F. D., Forsthoefel A. N., Lawther R. P., Kamalanathan L., Ritchie B. W., Latimer K. S., Lukert P. D.. 1998; Beak and feather disease virus and porcine circovirus genomes: intermediates between the geminiviruses and plant circoviruses. Archives of Virology143:1723–1744
    [Google Scholar]
  25. Noteborn M. H. M., Koch G.. 1995; Chicken anemia virus infection: molecular basis of pathogenicity. Avian Pathology24:11–31
    [Google Scholar]
  26. Nuovo G. J., Lidonnici K., MacConnell P., Lane B.. 1993; Intracellular localization of polymerase chain reaction (PCR)-amplified hepatitis C cDNA. American Journal of Surgery and Pathology17:683–690
    [Google Scholar]
  27. Plummer P. J., Alefantis T., Kaplan S., O’Connell P., Shawky S., Schat K. A.. 1998; Detection of duck enteritis virus by polymerase chain reaction. Avian Diseases42:554–564
    [Google Scholar]
  28. Pope C. R.. 1991; Chicken anemia agent. Veterinary Immunology and Immunopathology30:51–65
    [Google Scholar]
  29. Renshaw R. W., Soiné C., Weinkle T., O’Connell P. H., Ohashi K., Watson S., Lucio B., Harrington S., Schat K. A.. 1996; A hypervariable region in VP1 of chicken infectious anemia virus mediates rate of spread and cell tropism in tissue culture. Journal of Virology70:8872–8878
    [Google Scholar]
  30. Ritchie B. W., Niagro F. D., Lukert P. D., Steffens W. L., Latimer K. S.. 1989; Characterization of a new virus from cockatoos with psittacine beak and feather disease. Virology171:83–88
    [Google Scholar]
  31. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Analysis and cloning of eukaryotic genomic DNA. In Molecular Cloning: A Laboratory Manual vol 2, 2nd edn.pp. 9.1–9.62 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  32. Smyth J. A., Moffett D. A., McNulty M. S., Todd D., Mackie D. P.. 1993; A sequential histopathologic and immunocytochemical study of chicken anemia virus infection at one day of age. Avian Diseases37:324–338
    [Google Scholar]
  33. Soiné C., Watson S. K., Rybicki E., Lucio B., Nordgren R. M., Parrish C. R., Schat K. A.. 1993; Determination of the detection limit of the polymerase chain reaction for chicken infectious anemia virus. Avian Diseases37:467–476
    [Google Scholar]
  34. Studdert M. J.. 1993; Circoviridae: new viruses of pigs, parrots and chickens. Australian Veterinary Journal70:121–122
    [Google Scholar]
  35. Taniguchi T., Yuasa N., Maeda M., Horiuchi T.. 1983; Chronological observations on hemato-pathological changes in chicks inoculated with chicken anemia agent. National Institute of Animal Health Quarterly (Japan)23:1–12
    [Google Scholar]
  36. Tischer I., Gelderblom H., Vettermann W., Koch M. A.. 1982; A very small porcine virus with circular single-stranded DNA. Nature295:64–66
    [Google Scholar]
  37. Vielitz E., Landgraf H.. 1988; Anaemia-dermatitis of broilers: field observations on its occurrence, transmission and prevention. Avian Pathology17:113–120
    [Google Scholar]
  38. Von Bülow V., Witt M.. 1986; Vermehrung des Erregers der aviären infektiösen Anämie (CAA) in embryonierten Hühnereieren. Journal of Veterinary Medicine, Series B33:664–669
    [Google Scholar]
  39. Von Bülow V., Schat K. A.. 1997; Infectious anemia. In Diseases of Poultry pp739–756 Edited by Calnek B. W., Barnes H. J., Beard C. W., McDougald L. R., Saif Y. M. Ames, IA: Iowa State University Press;
    [Google Scholar]
  40. Yuasa N., Yoshida I.. 1983; Experimental egg transmission of chicken anemia agent. National Institute of Animal Health Quarterly (Japan23:99–100
    [Google Scholar]
  41. Yuasa N., Taniguchi T., Yoshida I.. 1979; Isolation and characteristics of an agent inducing anemia in chicks. Avian Diseases23:366–385
    [Google Scholar]
  42. Yuasa N., Taniguchi T., Imada T., Hihara H.. 1983; Distribution of chicken anemia agent (CAA) and detection of neutralizing antibody in chicks experimentally inoculated with CAA. National Institute of Animal Health Quarterly (Japan)23:78–81
    [Google Scholar]
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