1887

Abstract

In this study the protective effects of a live attenuated bovine leukaemia provirus (pBLVDX) with deletion in the R3 and G4 genes were tested. Six out of six sheep appeared to resist challenge with parental BLV344. Two out of three animals transfected with pBLVDX were protected against challenge with bovine leukaemia virus (BLV) from a naturally infected cow. As a model for the protection against infection by members of the human T-lymphotropic virus/BLV group, these data provide evidence that a DNA-based vaccination with an attenuated provirus is able to protect against challenge infections.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-4-965
2000-04-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/4/0810965a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-4-965&mimeType=html&fmt=ahah

References

  1. Daniel, M. F., Kirchhoff, F., Czajak, S. C., Sehgal, P. K. & Desrosiers, R. C. ( 1992; ). Protective effects of a live attenuated SIV vaccine with a deletion in the nef gene. Science 258, 1938-1941.[CrossRef]
    [Google Scholar]
  2. Desrosiers, R. C. ( 1992; ). HIV with multiple gene deletions as a live attenuated vaccine for AIDS. AIDS Research & Human Retroviruses 8, 411-420.[CrossRef]
    [Google Scholar]
  3. Desrosiers, R. C., Lifson, J. D., Gibbs, D., Czajak, S. C., Howe, A. Y. M., Arthur, L. O. & Johnson, R. P. ( 1998; ). Identification of highly attenuated mutants of simian immunodeficiency virus. Journal of Virology 72, 1431-1437.
    [Google Scholar]
  4. Hosie, M. J., Flynn, J. N., Rigby, A. A., Cannon, C., Dunsford, T., Mackay, N. A., Argyle, D., Willett, B. J., Miyazawa, T., Onions, D. E., Jarrett, O. & Neil, J. C. ( 1998; ). DNA vaccination affords significant protection against feline immunodeficiency virus infection without inducing detectable antiviral antibodies. Journal of Virology 72, 7310-7319.
    [Google Scholar]
  5. Kerkhofs, P., Heremans, H., Burny, A., Kettmann, R. & Willems, L. ( 1998; ). In vitro and in vivo oncogenic potential of bovine leukemia virus G4 protein. Journal of Virology 72, 2554-2559.
    [Google Scholar]
  6. Kerkhofs, P., Gatot, J.-S., Knapen, K., Mammerickx, M., Birny, A., Portetelle, D., Willems, L. & Kettmann, R. ( 2000; ). Long-term protection against bovine leukaemia virus replication in cattle and sheep. Journal of General Virology 81, 957-963.
    [Google Scholar]
  7. Langlois, A. J., Desrosiers, R. C., Lewis, M. G., Kewalramani, V. H., Littman, D. R., Zhou, J. Y., Manson, K., Wyand, M. S., Bolognesi, D. P. & Montefiori, D. C. ( 1998; ). Neutralizing antibodies in sera from macaques immunized with attenuated simian immunodeficiency virus. Journal of Virology 72, 6950-6955.
    [Google Scholar]
  8. Lassauzet, M., Johnson, W., Thurmond, M. & Stevens, F. ( 1989; ). Protection of colostral antibodies against bovine leukemia virus infection in calves on a California dairy. Canadian Journal of Veterinary Research 53, 424-430.
    [Google Scholar]
  9. Lu, Y., Salvato, M. S., Pauza, C. D., Li, J., Sodroski, J., Manson, K., Wyand, M. S., Letvin, N., Jenkins, S., Touzian, N., Chutkowski, C., Kushner, N., LeFaile, M., Payne, G. L. & Roberts, B. ( 1996; ). Utility of SHIV for testing HIV-1 vaccine candidates in macaques. Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology 12, 99-106.[CrossRef]
    [Google Scholar]
  10. Mammerickx, M., Portetelle, D., Burny, A. & Leunen, J. ( 1980; ). Detection by immunodiffusion – and radioimmunoassay – tests of antibodies to bovine leukemia virus antigens in sera of experimentally infected sheep and cattle. Zentralblatt für Veterinärmedizin, Reihe B 27, 291-303.
    [Google Scholar]
  11. Mammerickx, M., Portetelle, D., De Clerco, K. & Burny, A. ( 1987; ). Experimental transmission of enzootic bovine leukosis to cattle, sheep and goats: infectious doses of blood and incubation period of the disease. Leukemia Research 11, 353-358.[CrossRef]
    [Google Scholar]
  12. Ohishi, K., Suzuki, H., Yamamoto, T., Maruyama, T., Miki, K., Ikawa, Y., Numakunai, S., Okada, K., Ohshima, K. & Sugimoto, M. ( 1991; ). Protective immunity against bovine leukaemia virus (BLV) induced in carrier sheep by inoculation with a vaccinia–BLV env recombinant: association with cell-mediated immunity. Journal of General Virology 72, 1887-1892.[CrossRef]
    [Google Scholar]
  13. Ohishi, K., Suzuki, H., Yasutomi, Y., Onima, M., Okada, K., Numakumai, S., Ohsima, K., Ikawa, Y. & Sugimoto, M. ( 1992; ). Augmentation of bovine leukaemia virus (BLV)-specific lymphocyte proliferation responses in ruminant by inoculation with BLV env-recombinant vaccinia virus: the role in the suppression of BLV replication. Microbiology and Immunology 36, 1317-1323.[CrossRef]
    [Google Scholar]
  14. Portetelle, D., Callebaut, I., Bex, F. & Burny, A. ( 1993; ). Vaccination against animal retroviruses. In Progress in Vaccinology, pp. 87-138. Edited by R. Pandey, S. Hoglund & G. Prassad. Berlin: Springer-Verlag.
  15. Reichert, M. & Grundboeck-Juśko, J. ( 1991; ). Molecular cloning of provirus DNA from bovine leukaemia lymphocytes and its application as a probe for diagnostic purpose. Acta Biochimica Polonica 38, 111-114.
    [Google Scholar]
  16. Sagata, N., Yasunaga, Y., Tsuzuku-Kawamura, J., Ohishi, K., Ogawa, Y. & Ikawa, Y. ( 1985; ). Complete nucleotide sequence of the genome of bovine leukemia virus: its evolutionary relationship to other retroviruses. Proceedings of the National Academy of Sciences, USA 82, 677-681.[CrossRef]
    [Google Scholar]
  17. Shibata, R., Simeon, C., Czajak, S. C., Desrosiers, R. C. & Martin, A. M. ( 1997; ). Live, attenuated simian immunodeficiency virus vaccines elicit potent resistance against a challenge with a human immunodeficiency virus type 1 chimeric virus. Journal of Virology 71, 8141-8148.
    [Google Scholar]
  18. Van den Broeke, A., Cleuter, Y., Chen, G., Portetelle, D., Mammerickx, M., Zagury, D., Fouchard, M., Coulombel, L., Kettmann, R. & Burny, A. ( 1988; ). Even transcriptionally competent proviruses are silent in bovine leukemia virus-infected sheep tumor cells. Proceedings of the National Academy of Sciences, USA 85, 9263-9267.[CrossRef]
    [Google Scholar]
  19. Willems, L., Kettmann, R., Dequiedt, F., Portetelle, D., Voneche, V., Cornil, L., Kerkhofs, P., Burny, A. & Mammerickx, M. ( 1993; ). In vivo infection of sheep by bovine leukemia virus mutants. Journal of Virology 67, 4078-4085.
    [Google Scholar]
  20. Willems, L., Kerkhofs, P., Dequiedt, F., Portetelle, D., Mammerickx, M., Burny, A. & Kettmann, R. ( 1994; ). Attenuation of bovine leukemia virus by deletion of R3 and G4 open reading frames. Proceedings of the National Academy of Sciences, USA 91, 11532-11536.[CrossRef]
    [Google Scholar]
  21. Willems, L., Gatot, J. S., Mammerickx, M., Portetelle, D., Burny, A., Kerkohofs, P. & Kettmann, R. ( 1995; ). The YXXL signalling motifs of the bovine leukemia transmembrane protein are required for in vivo infection and maintenance of high viral loads. Journal of Virology 69, 4137-4141.
    [Google Scholar]
  22. Willems, L., Kerkhofs, P., Attenelle, L., Burny, A., Portetelle, D. & Kettmann, R. ( 1997; ). The major homology region of bovine leukemia virus p24 gag is required for virus infectivity in vivo. Journal of General Virology 78, 637-640.
    [Google Scholar]
  23. Wyand, M. S., Manson, K. H., Garcia-Moll, M., Montefiori, D. C. & Desrosiers, R. C. ( 1996; ). Vaccine protection by a triple deletion mutant of simian immunodeficiency virus. Journal of Virology 70, 3724-3733.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-4-965
Loading
/content/journal/jgv/10.1099/0022-1317-81-4-965
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error