1887

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Volume 81, Issue 12

Characterization of repetitive DNA regions and methylated DNA in ascovirus genomes Free

Abstract

The accompanying phylogenetic study of large double-stranded DNA viruses based on their δ DNA polymerase genes suggests that ascoviruses (family ) and iridoviruses (family ) are closely related and may share a common ancestor. This relationship was unexpected because of marked differences between these viruses. Iridoviruses produce icosahedral virions and occur broadly among vertebrates and invertebrates, whereas ascoviruses typically produce reniform or bacilliform virions and are restricted to insect hosts, primarily lepidopterans. Detailed comparisons of these two virus types are not possible because fundamental information on the properties of the virions and their genomes is lacking, especially for ascoviruses. To facilitate further investigation of the putative evolutionary relationship between ascoviruses and iridoviruses, the genomes of representative viruses from each family were compared with respect to physical configuration, presence of DNA repeats and degree of DNA methylation. Genomes from (SfAV1), (HvAV3) and (DpAV4) ascoviruses were all found to be circular and partially superhelical and to contain large interspersed repeats of 1–3 kbp. Mosquito (IV type 3), lepidopteran (IV type 6) and isopod (IV type 31) iridovirus genomes were all linear and lacked large regions of repetitive DNA. Ascovirus and iridovirus genomes were methylated and one, DpAV4, had the highest degree of methylation of any reported animal DNA virus. The major differences in the physical and biochemical characteristics of ascoviruses and iridoviruses reported here provide a foundation for further studies of their relatedness while making their possible close relationship and divergence during evolution of even greater interest.

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© Microbiology Society
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2000-12-01
2024-04-26
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References

  1. Afonso C. L., Tulman E. R., Lu Z., Oma E., Kutish G. F., Rock D. L. 1999; The genome of Melanoplus sanguinipes entomopoxvirus. Journal of Virology 73:533–552
     [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1994 Current Protocols in Molecular Biology vols 1 and 2 New York: John Wiley;
     [Google Scholar]
  3. Ayres M. D., Howard S. C., Kusio J., Lopez-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605
     [Google Scholar]
  4. Bigot Y., Rabouille A., Sizaret P.-Y., Hamelin M.-H., Periquet G. 1997a; Particle and genomic characteristics of a new member of the Ascoviridae : Diadromus pulchellus ascovirus. Journal of General Virology 78:1139–1147
     [Google Scholar]
  5. Bigot Y., Rabouille A., Doury G., Sizaret P.-Y., Delbost F., Hamelin M.-H., Periquet G. 1997b; Biological and molecular features of the relationships between Diadromus pulchellus ascovirus, a parasitoid hymenopteran wasp ( Diadromus pulchellus ) and its lepidopteran host, Acrolepiopsis assectella. Journal of General Virology 78:1149–1163
     [Google Scholar]
  6. Britten R. J., Graham D. E., Neufeld B. R. 1974; Analysis of repeating DNA sequences by reassociation. Methods in Enzymology 29:363–418
     [Google Scholar]
  7. Cheng X.-W., Carner G. R., Brown T. M. 1999; Circular configuration of the genome of ascoviruses. Journal of General Virology 80:1537–1540
     [Google Scholar]
  8. Delius H., Darai G., Flügel R. M. 1984; DNA analysis of insect iridescent virus 6: evidence for circular permutation and terminal redundancy. Journal of Virology 49:609–614
     [Google Scholar]
  9. Dessen P., Fondrat C., Valencien C., Mugnier C. 1990; BISANCE: a French service for access to biomolecular sequence databases. Computer Applications in the Biosciences 6:355–356
     [Google Scholar]
  10. Domingo E., Escarmis C., Menendez-Arias L., Holland J. J. 1999; Viral quasispecies and fitness variations. In Origin and Evolution of Viruses pp 141–161 Edited by Domingo E., Webster R., Holland J. San Diego & London: Academic Press;
     [Google Scholar]
  11. Federici B. A., Vlak J. M., Hamm J. J. 1990; Comparative study of virion structure, protein composition and genomic DNA of three ascovirus isolates. Journal of General Virology 71:1661–1668
     [Google Scholar]
  12. Federici B. A., Bigot Y., Granados R. R., Hamm J. J., Miller L. K., Vlak J. M. 2000; Family Ascoviridae. In Virus Taxonomy: Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses Edited by Regenmortel M. H. V. Van, Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., McGeoch D. J., Maniloff J., Mayo M. A., Pringle C. R., Wickner R. B. San Diego: Academic Press;
     [Google Scholar]
  13. Gehrke C. W., McCune R. A., Gama-Sosa M. A., Ehrlich M., Kuo K. C. 1984; Quantitative reversed-phase high-performance liquid chromatography of major and modified nucleosides in DNA. Journal of Chromatography 301:199–219
     [Google Scholar]
  14. Goorha R., Murti K. G. 1982; The genome of frog virus 3, an animal DNA virus, is circularly permuted and terminally redundant. Proceedings of the National Academy of Sciences, USA 79:248–252
     [Google Scholar]
  15. Goorha R., Granoff A., Willis D. B., Murti K. G. 1984; The role of DNA methylation in virus replication: inhibition of frog virus 3 replication by 5-azacytidine. Virology 138:94–102
     [Google Scholar]
  16. Hamm J. J., Nordlund D. A., Marti O. G. 1985; Effects of the nonoccluded virus of Spodoptera frugiperda (Lepidoptera: Noctuidae) on the development of a parasitoid, Cotesia marginiventris (Hymenoptera: Braconidae). Environmental Entomology 14:258–261
     [Google Scholar]
  17. Jabbari K., Caccio S., Païs de Barros J. P., Desgrès J., Bernardi G. 1997; Evolutionary changes in CpG and methylation levels in the genomes of vertebrates. Gene 205:109–118
     [Google Scholar]
  18. Karlin S., Burge C. 1995; Dinucleotide relative abundance extremes: a genomic signature. Trends in Genetics 11:283–290
     [Google Scholar]
  19. Kaur K., Rohozinski J., Goorha R. 1995; Identification and characterization of the frog virus 3 DNA methyltransferase gene. Journal of General Virology 76:1937–1943
     [Google Scholar]
  20. Regev A., Lamb M. J., Jablonka E. 1998; The role of DNA methylation in invertebrates: developmental regulation or genome defense?. Molecular Biology & Evolution 15:880–891
     [Google Scholar]
  21. Schnitzler P., Soltau J. B., Fischer M., Reisner H., Scholz J., Delius H., Darai G. 1987a; Molecular cloning and physical mapping of the genome of insect iridescent virus type 6: further evidence for circular permutation of the viral genome. Virology 160:66–74
     [Google Scholar]
  22. Schnitzler P., Delius H., Scholz J., Touray M., Orth E., Darai G. 1987b; Identification and nucleotide sequence analysis of the repetitive DNA element in the genome of fish lymphocystis disease virus. Virology 161:570–578
     [Google Scholar]
  23. Stasiak K., Demattei M.-V., Federici B. A., Bigot Y. 2000; Phylogenetic position of the Diadromus pulchellus ascovirus DNA polymerase among viruses with large double-stranded DNA genomes. Journal of General Virology 81:3059–3072
     [Google Scholar]
  24. Summers M. D., Anderson D. L. 1972; Characterization of deoxyribonucleic acid isolated from the granulosis viruses of the cabbage looper, Trichoplusia ni and the fall armyworm, Spodoptera frugiperda. Virology 50:459–471
     [Google Scholar]
  25. Tidona C. A., Darai G. 1997; The complete DNA sequence of lymphocystis disease virus. Virology 230:207–216
     [Google Scholar]
  26. Van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., McGeoch D. J., Maniloff J., Mayo M. A., Pringle C. R., Wickner R. B. (editors) 2000; Virus Taxonomy Classification and Nomenclature of Viruses. Seventh Report of the International Committee on Taxonomy of Viruses San Diego: Academic Press;
     [Google Scholar]
  27. Wagner H., Simon D., Werner E., Gelderblom H., Darai G., Flügel R. M. 1985; Methylation pattern of fish lymphocystis disease virus DNA. Journal of Virology 53:1005–1007
     [Google Scholar]
  28. Williams T. 1994; Comparative studies of iridoviruses: further support for a new classification. Virus Research 33:99–121
     [Google Scholar]
  29. Willis D. B., Granoff A. 1980; Frog virus 3 DNA is heavily methylated at CpG sequences. Virology 107:250–257
     [Google Scholar]
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Characterization of repetitive DNA regions and methylated DNA in ascovirus genomes
J. Gen. Virol. 81, 3073 (2000); https://doi.org/10.1099/0022-1317-81-12-3073
/content/journal/jgv/10.1099/0022-1317-81-12-3073
/content/journal/jgv/10.1099/0022-1317-81-12-3073
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