1887

Abstract

Human astroviruses are an important cause of gastroenteritis. As part of a molecular epidemiological study carried out in Mexico a human astrovirus isolate, Yuc-8, was adapted to grow in CaCo-2 cells, and its entire genome was sequenced. A 15 amino acid deletion in ORF1a, which has been associated with adaptation of astroviruses to grow in cells other than CaCo-2, was present in Yuc-8. Comparative sequence analysis of the Yuc-8 ORF2 with reported human astrovirus sequences revealed that this isolate belongs to genotype (serotype) 8. Two distinct domains in ORF2 were observed: an amino-terminal domain (residues 1 to 415), with identities higher than 81% among the strains analysed, and a carboxy-terminal domain (residues 416 to 782) with identities between 36 and 60%. Two non-superimposable phylogenetic trees were generated by separate analysis of these two domains, suggesting that a differential selective pressure is exerted along the structural polyprotein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-12-2891
2000-12-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/12/0812891a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-12-2891&mimeType=html&fmt=ahah

References

  1. Bass, D. & Qiu, S. ( 2000; ). Proteolytic processing of the astrovirus capsid. Journal of Virology 74, 1810-1814.[CrossRef]
    [Google Scholar]
  2. Bass, D. & Upadhyayula, U. ( 1997; ). Characterization of human serotype 1 astrovirus-neutralizing epitopes. Journal of Virology 71, 8666-8671.
    [Google Scholar]
  3. Belliot, G., Laveran, H. & Monroe, S. ( 1997; ). Detection and genetic differentiation of human astroviruses: phylogenetic grouping varies by coding region. Archives of Virology 142, 1323-1334.[CrossRef]
    [Google Scholar]
  4. Gaggero, A., O’Ryan, M., Noel, J., Glass, R., Monroe, S., Mamani, N., Prado, V. & Avendano, L. ( 1998; ). Prevalence of astrovirus infection among Chilean children with acute gastroenteritis. Journal of Clinical Microbiology 36, 3691-3693.
    [Google Scholar]
  5. Herrmann, J., Hudson, R., Perron-Henry, D., Kurtz, J. & Blacklow, N. ( 1988; ). Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies. Journal of Infectious Diseases 158, 182-185.[CrossRef]
    [Google Scholar]
  6. Herrmann, J., Cubitt, W., Hudson, R., Perron-Henry, D., Oshiro, L. & Blacklow, N. ( 1990; ). Immunological characterization of the Marin County strain of astrovirus. Archives of Virology 110, 213-220.[CrossRef]
    [Google Scholar]
  7. Herrmann, J., Taylor, D., Echeverria, P. & Blacklow, N. ( 1991; ). Astroviruses as a cause of gastroenteritis in children. New England Journal of Medicine 324, 1757-1760.[CrossRef]
    [Google Scholar]
  8. Jiang, B., Monroe, S., Koonin, E., Stine, S. & Glass, R. ( 1993; ). RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis. Proceedings of the National Academy of Sciences, USA 90, 10539-10543.[CrossRef]
    [Google Scholar]
  9. Jonassen, C., Jonassen, T. & Grinde, B. ( 1998; ). A common RNA motif in the 3′ end of the genomes of astroviruses, avian infectious bronchitis virus and an equine rhinovirus. Journal of General Virology 79, 715-718.
    [Google Scholar]
  10. Lewis, T. & Matsui, S. ( 1997; ). Studies of the astrovirus signal that induces (−1) ribosomal frameshifting. Advances in Experimental Medicine Biology 412, 323-330.
    [Google Scholar]
  11. Lopez, S., Arias, C., Mendez, E. & Espejo, R. ( 1986; ). Conservation in rotaviruses of the protein region containing the two sites associated with trypsin enhancement of infectivity. Virology. 154, 224-7.[CrossRef]
    [Google Scholar]
  12. Marczinke, B., Bloys, A., Brown, T., Willcocks, M., Carter, M. & Brierley, I. ( 1994; ). The human astrovirus RNA-dependent RNA polymerase coding region is expressed by ribosomal frameshifting. Journal of Virology 68, 5588-5595.
    [Google Scholar]
  13. Monceyron, C., Grinde, B. & Jonassen, T. ( 1997; ). Molecular characterization of the 3′-end of the astrovirus genome. Archives of Virology 142, 699-706.[CrossRef]
    [Google Scholar]
  14. Monroe, S., Stine, S., Gorelkin, L., Herrmann, J., Blacklow, N. & Glass, R. ( 1991; ). Temporal synthesis of proteins and RNAs during human astrovirus infection of cultured cells. Journal of Virology 65, 641-648.
    [Google Scholar]
  15. Monroe, S., Jiang, B., Stine, S., Koopmans, M. & Glass, R. ( 1993; ). Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses. Journal of Virology 67, 3611-3614.
    [Google Scholar]
  16. Mustafa, H., Palombo, E. & Bishop, R. ( 2000; ). Epidemiology of astrovirus Infection in young children hospitalized with acute gastroenteritis in Melbourne, Australia, over a period of four consecutive years, 1995 to 1998. Journal of Clinical Microbiology 38, 1058-1062.
    [Google Scholar]
  17. Noel, J. & Cubitt, D. ( 1994; ). Identification of astrovirus serotypes from children treated at the Hospitals for Sick Children, London 1981–93. Epidemiology and Infection 113, 153-159.[CrossRef]
    [Google Scholar]
  18. Noel, J., Lee, T., Kurtz, J., Glass, R. & Monroe, S. ( 1995; ). Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing. Journal of Clinical Microbiology 33, 797-801.
    [Google Scholar]
  19. Palombo, E. & Bishop, R. ( 1996; ). Annual incidence, serotype distribution, and genetic diversity of human astrovirus isolates from hospitalized children in Melbourne, Australia. Journal of Clinical Microbiology 34, 1750-1753.
    [Google Scholar]
  20. Saito, K., Ushijima, H., Nishio, O., Oseto, M., Motohiro, H., Ueda, Y., Takagi, M., Nakaya, S., Ando, T., Glass, R. and others ( 1995; ). Detection of astroviruses from stool samples in Japan using reverse transcription and polymerase chain reaction amplification. Microbiology and Immunology 39, 825–828.[CrossRef]
    [Google Scholar]
  21. Sanchez-Fauquier, A., Carrascosa, A., Carrascosa, J., Otero, A., Glass, R., Lopez, J., Martin, C. S. & Melero, J. ( 1994; ). Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization. Virology 201, 312-320.[CrossRef]
    [Google Scholar]
  22. Shastri, S., Doane, A., Gonzales, J., Upadhyayula, U. & Bass, D. ( 1998; ). Prevalence of astroviruses in a children’s hospital. Journal of Clinical Microbiology 36, 2571-2574.
    [Google Scholar]
  23. Willcocks, M., Ashton, N., Kurtz, J., Cubitt, W. & Carter, M. ( 1994a; ). Cell culture adaptation of astrovirus involves a deletion. Journal of Virology 68, 6057-6058.
    [Google Scholar]
  24. Willcocks, M., Brown, T., Madeley, C. & Carter, M. ( 1994b; ). The complete sequence of a human astrovirus. Journal of General Virology 75, 1785-1788.[CrossRef]
    [Google Scholar]
  25. Willcocks, M., Kurtz, J., Lee, T. W. & Carter, M. ( 1995; ). Prevalence of human astrovirus serotype 4: capsid protein sequence and comparison with other strains. Epidemiology and infection 114, 385-394.[CrossRef]
    [Google Scholar]
  26. Willcocks, M., Boxall, A. & Carter, M. ( 1999; ). Processing and intracellular location of human astrovirus non-structural proteins. Journal of General Virology 80, 2607-2611.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-12-2891
Loading
/content/journal/jgv/10.1099/0022-1317-81-12-2891
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error