1887

Abstract

Sequence analysis was performed of all or part of the genes encoding the fusion (F), polymerase (L) and attachment (G) proteins of two French non-A/non-B avian pneumovirus (APV) isolates (Fr/85/1 and Fr/85/2). The two isolates shared at least 99·7% nt and 99·0% aa sequence identity. Comparison with the F genes from subgroup A, subgroup B or Colorado APVs revealed nt and aa identities of 70·0–80·5% and 77·6–97·2%, respectively, with the L gene sharing 76·1% nt and 85·3% aa identity with that of a subgroup A isolate. The Fr/85/1 and Fr/85/2 G genes comprised 1185 nt, encoding a protein of 389 aa. Common features with subgroup A and subgroup B G proteins included an amino-terminal membrane anchor, a high serine and threonine content, conservation of cysteine residues and a single extracellular region of highly conserved sequence proposed to be the functional domain involved in virus attachment to cellular receptors. However, the Fr/85/1 and Fr/85/2 G sequences shared at best 56·6% nt and 31·2% aa identity with subgroup A and B APVs, whereas these isolates share 38% aa identity. Phylogenetic analysis of the F, G and L genes of pneumoviruses suggested that isolates Fr/85/1 and Fr/85/2 belong to a previously unrecognized APV subgroup, tentatively named D. G-based oligonucleotide primers were defined for the specific molecular identification of subgroup D. These are the first G protein sequences of non-A/non-B APVs to be determined.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-11-2723
2000-11-01
2021-05-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/11/0812723a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-11-2723&mimeType=html&fmt=ahah

References

  1. Akerlind-Stopner B., Utter G., Mufson M. A., Orvell C., Lerner R. A., Norrby E. 1990; A subgroup-specific antigenic site in the G protein of respiratory syncytial virus forms a disulfide-bonded loop. Journal of Virology 64:5143–5148
    [Google Scholar]
  2. Alexander D. J. 1997; Newcastle disease and other avian Paramyxoviridae infections. In Diseases of Poultry pp 541–569 Edited by Calnek B. W. Ames, IA: Iowa State University Press;
    [Google Scholar]
  3. Anderson L. J., Hierholzer J. C., Tsou C., Hendry R. M., Fernie B. F., Stone Y., Mclntosh K. 1985; Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. Journal of Infectious Diseases 151:626–633
    [Google Scholar]
  4. Bäyon-Auboyer M.-H., Jestin V., Toquin D., Cherbonnel M., Eterradossi N. 1999; Comparison of F-, G- and N-based RT–PCR protocols with conventional virological procedures for the detection and typing of turkey rhinotracheitis virus. Archives of Virology 144:1091–1109
    [Google Scholar]
  5. Cane P. A., Pringle C. R. 1995; Evolution of subgroup A respiratory syncytial virus: evidence for progressive accumulation of amino acid changes in the attachment protein. Journal of Virology 69:2918–2925
    [Google Scholar]
  6. Cane P. A., Matthews D. A., Pringle C. R. 1991; Identification of variable domains of the attachment (G) protein of subgroup A respiratory syncytial viruses. Journal of General Virology 72:2091–2096
    [Google Scholar]
  7. Cavanagh D., Barrett T. 1988; Pneumovirus-like characteristics of the mRNA and proteins of turkey rhinotracheitis virus. Virus Research 11:241–256
    [Google Scholar]
  8. Collins M. S., Gough R. E. 1988; Characterization of a virus associated with turkey rhinotracheitis. Journal of General Virology 69:909–916
    [Google Scholar]
  9. Collins M. S., Gough R. E., Lister S. A., Chettle N., Eddy R. 1986; Further characterisation of a virus associated with turkey rhinotracheitis. Veterinary Record 119:606
    [Google Scholar]
  10. Collins M. S., Gough R. E., Alexander D. J. 1993; Antigenic differentiation of avian pneumovirus isolates using polyclonal antisera and mouse monoclonal antibodies. Avian Pathology 22:469–479
    [Google Scholar]
  11. Collins P. L., McIntosh K., Chanock R. M. 1996; Respiratory syncytial virus. In Fields Virology pp 1313–1351 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  12. Cook J. K. A., Jones B. V., Ellis M. M., Jing L., Cavanagh D. 1993; Antigenic differentiation of strains of turkey rhinotracheitis virus using monoclonal antibodies. Avian Pathology 22:257–273
    [Google Scholar]
  13. Cook J. K. A., Huggins M. B., Senne D. A. 1998; Antigenic variation within avian pneumovirus. In Proceedings of an International Symposium on Infectious Bronchitis and Pneumovirus Infections in Poultry Rauschholzhauzen, Germany: pp 9–13 Edited by Kaleta E. F., Heffels-Redmann U. Geissen, Germany: Institut für Geflugelkrankheiten;
    [Google Scholar]
  14. Eterradossi N., Toquin D., Guittet M., Bennejean G. 1995; Evaluation of different turkey rhinotracheitis viruses used as antigens for serological testing following live vaccination and challenge. Journal of Veterinary Medicine Series B 42:175–186
    [Google Scholar]
  15. Felsenstein J. 1993 PHYLIP (phylogenetic inference package), version 3.52c Department of Genetics, University of Washington; Seattle, WA, USA:
    [Google Scholar]
  16. Garcia O., Martin M., Dopazo J., Arbiza J., Frabasile S., Russi J., Hortal M., Perez-Brena P., Martinez I., Garcia-Barreno B., Melero J. A. 1994; Evolutionary pattern of human respiratory syncytial virus (subgroup A): cocirculating lineages and correlation of genetic and antigenic changes in the G glycoprotein. Journal of Virology 68:5448–5459
    [Google Scholar]
  17. Giraud P., Bennejean G., Guittet M., Toquin D. 1986; A possible viral candidate for the aetiology of turkey rhinotracheitis. Veterinary Record 118:81
    [Google Scholar]
  18. Gruber C., Levine S. 1985; Respiratory syncytial virus polypeptides. IV. The oligosaccharides of the glycoproteins. Journal of General Virology 66:417–432
    [Google Scholar]
  19. Hafez H. M. 1992; Comparative investigation on different turkey rhinotracheitis (TRT) virus isolates from different countries. Deutsche Tierärtzliche Wochenschrift 99:486–488
    [Google Scholar]
  20. Hubbard S. C., Ivatt R. J. 1981; Synthesis and processing of asparagine-linked oligosaccharides. Annual Review of Biochemistry 50:555–583
    [Google Scholar]
  21. Jentoft N. 1990; Why are proteins O-glycosylated?Trends in Biochemical Sciences. 15291–294
  22. Johnson P. R., Spriggs M. K., Olmsted R. A., Collins P. L. 1987; The G glycoprotein of human respiratory syncytial viruses of subgroups A and B: extensive sequence divergence between antigenically related proteins. Proceedings of the National Academy of Sciences, USA 84:5625–5629
    [Google Scholar]
  23. Juhasz K., Easton A. J. 1994; Extensive sequence variation in the attachment (G) protein gene of avian pneumovirus: evidence for two distinct subgroups. Journal of General Virology 75:2873–2880
    [Google Scholar]
  24. Lambert D. M. 1988; Role of oligosaccharides in the structure and function of respiratory syncytial virus glycoproteins. Virology 164:458–466
    [Google Scholar]
  25. Langedijk J. P. M., Schaaper W. M. M., Meloen R. H., van Oirschot J. T. 1996; Proposed three-dimensional model for the attachment protein G of respiratory syncytial virus. Journal of General Virology 77:1249–1257
    [Google Scholar]
  26. Langedijk J. P. M., Meloen R. H., Taylor G., Furze J. M., van Oirschot J. T. 1997; Antigenic structure of the central conserved region of protein G of bovine respiratory syncytial virus. Journal of Virology 71:4055–4061
    [Google Scholar]
  27. Li J., Ling R., Randhawa J. S., Shaw K., Davis P. J., Juhasz K., Pringle C. R., Easton A. J., Cavanagh D. 1996; Sequence of the nucleocapsid protein gene of subgroup A and B avian pneumoviruses. Virus Research 41:185–191
    [Google Scholar]
  28. Ling R., Easton A. J., Pringle C. R. 1992; Sequence analysis of the 22K, SH and G genes of turkey rhinotracheitis virus and their intergenic regions reveals a gene order different from that of other pneumoviruses. Journal of General Virology 73:1709–1715
    [Google Scholar]
  29. McDougall J. S., Cook J. K. A. 1986; Turkey rhinotracheitis: preliminary investigations. Veterinary Record 118:206–207
    [Google Scholar]
  30. Melero J. A., García-Barreno B., Martínez I., Pringle C. R., Cane P. A. 1997; Antigenic structure, evolution and immunobiology of human respiratory syncytial virus attachment (G) protein. Journal of General Virology 78:2411–2418
    [Google Scholar]
  31. Mufson M. A., Örvell C., Rafnar B., Norrby E. 1985; Two distinct subtypes of human respiratory syncytial virus. Journal of General Virology 66:2111–2124
    [Google Scholar]
  32. Naylor C. J., Jones R. C. 1993; Turkey rhinotracheitis: a review. Veterinary Bulletin 63:439–449
    [Google Scholar]
  33. Naylor C., Shaw K., Britton P., Cavanagh D. 1997; Appearance of type B avian pneumovirus in Great Britain. Avian Pathology 26:327–338
    [Google Scholar]
  34. Naylor C. J., Britton P., Cavanagh D. 1998; The ectodomains but not the transmembrane domains of the fusion proteins of subtypes A and B avian pneumovirus are conserved to a similar extent as those of human respiratory syncytial virus. Journal of General Virology 79:1393–1398
    [Google Scholar]
  35. Palomo C., García-Barreno B., Peñas C., Melero J. A. 1991; The G protein of human respiratory syncytial virus: significance of carbohydrate side-chains and the C-terminal end to its antigenicity. Journal of General Virology 72:669–675
    [Google Scholar]
  36. Poch O., Blumberg B. M., Bougueleret L., Tordo N. 1990; Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. Journal of General Virology 71:1153–1162
    [Google Scholar]
  37. Pringle C. R. 1998; Virus taxonomy – San Diego 1998. Archives of Virology 143:1449–1459
    [Google Scholar]
  38. Randhawa J. S., Wilson S. D., Tolley K. P., Cavanagh D., Pringle C. R., Easton A. J. 1996; Nucleotide sequence of the gene encoding the viral polymerase of avian pneumovirus. Journal of General Virology 77:3047–3051
    [Google Scholar]
  39. Randhawa J. S., Marriott A. C., Pringle C. R., Easton A. J. 1997; Rescue of synthetic minireplicons establishes the absence of the NS1 and NS2 genes from avian pneumovirus. Journal of Virology 71:9849–9854
    [Google Scholar]
  40. Rueda P., Garcia-Barreno B., Melero J. A. 1994; Loss of conserved cysteine residues in the attachment (G) glycoprotein of two human respiratory syncytial virus escape mutants that contain multiple A–G substitutions (hypermutations. Virology 198:653–662
    [Google Scholar]
  41. Satake M., Coligan J. E., Elango N., Norrby E., Venkatesan S. 1985; Respiratory syncytial virus envelope glycoprotein (G) has a novel structure. Nucleic Acids Research 13:7795–7812
    [Google Scholar]
  42. Seal B. S. 1998; Matrix protein gene nucleotide and predicted amino acid sequence demonstrate that the first US avian pneumovirus isolate is distinct from European strains. Virus Research 58:45–52
    [Google Scholar]
  43. Senne D. A., Edson R. K., Pedersen J. C., Panigraphy B. 1997; Avian pneumovirus update. In Proceedings of the 134th Annual Congress of the American Veterinary Medical Association p 190 Reno, NV, USA:
  44. Simard C., Nadon F., Seguin C., Thien N. N., Binz H., Basso J., Laliberte J. F., Trudel M. 1997; Subgroup specific protection of mice from respiratory syncytial virus infection with peptides encompassing the amino acid region 174–187 from the G glycoprotein: the role of cysteinyl residues in protection. Vaccine 15:423–432
    [Google Scholar]
  45. Sullender W. M., Mufson M. A., Anderson L. J., Wertz G. W. 1991; Genetic diversity of the attachment protein of subgroup B respiratory syncytial viruses. Journal of Virology 65:5425–5434
    [Google Scholar]
  46. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
    [Google Scholar]
  47. Toquin D., Eterradossi N., Guittet M. 1992; Infectious rhinotracheitis in turkeys: antigenic differences revealed by ELISA. In Proceedings of the Seminar: New and Evolving Virus Diseases of Poultry pp 111–121 publication VI/41 04/94-EN, III-121 Edited by McNulty M., McFerran J. Brussels: European Commission;
    [Google Scholar]
  48. Toquin D., Eterradossi N., Guittet M. 1996; Use of a related ELISA antigen for efficient TRT serological testing following live vaccination. Veterinary Record 139:71–72
    [Google Scholar]
  49. Toquin D., Bäyon-Auboyer M.-H., Jestin V., Eterradossi N. 1999; Réponse sérologique et protection croisée vis à vis de l’infection par une souche NON-A NON-B du virus de la rhinotracheite infectieuse de la dinde. In Proceedings of the Troisièmes Journées de la Recherche Avicole pp. 221–223 Saint-Malo, France: 23–25 March 1999
  50. Toquin D., Bäyon-Auboyer M.-H., Senne D. A., Eterradossi N. 2000; Lack of antigenic relationship between French and recent North American non-A/non-B turkey rhinotracheitis virus. Avian Diseases (in press
    [Google Scholar]
  51. Tordo N., Poch O., Ermine A., Keith G., Rougeon F. 1988; Completion of the rabies virus genome sequence determination: highly conserved domains among the L (polymerase) proteins of unsegmented negative-strand RNA viruses. Virology 165:565–576
    [Google Scholar]
  52. Trudel M., Nadon F., Séguin C., Binz H. 1991; Protection of BALB/c mice from respiratory syncytial virus infection by immunization with a synthetic peptide derived from the G glycoprotein. Virology 185:749–757
    [Google Scholar]
  53. von Heijne G. 1992; Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. Journal of Molecular Biology 225:487–494
    [Google Scholar]
  54. Wertz G. W., Collins P. L., Huang Y., Gruber C., Levine S., Ball L. A. 1985; Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proceedings of the National Academy of Sciences, USA 82:4075–4079
    [Google Scholar]
  55. Yu Q., Davis P. J., Barrett T., Binns M. M., Boursnell M. E. G., Cavanagh D. 1991; Deduced amino acid sequence of the fusion glycoprotein of turkey rhinotracheitis virus has greater identity with that of human respiratory syncytial virus, a pneumovirus, than that of paramyxoviruses and morbilliviruses. Journal of General Virology 72:75–81
    [Google Scholar]
  56. Yu Q., Davis P. J., Brown T. D. K., Cavanagh D. 1992a; Sequence and in vitro expression of the M2 gene of turkey rhinotracheitis pneumovirus. Journal of General Virology 73:1355–1363
    [Google Scholar]
  57. Yu Q., Davis P. J., Li J., Cavanagh D. 1992b; Cloning and sequencing of the matrix protein (M) gene of turkey rhinotracheitis virus reveal a gene order different from that of respiratory syncytial virus. Virology 186:426–434
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-11-2723
Loading
/content/journal/jgv/10.1099/0022-1317-81-11-2723
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error