1887

Abstract

To study the biological relevance of using bovine herpesvirus-1 (BHV-1) as a vector for expressing cytokines, a BHV-1 virus that expressed bovine interferon-γ (IFN-γ) was constructed. This recombinant virus (BHV-1/IFNγ) was then used to infect the natural host in a respiratory disease model. characterization of the recombinant interferon-γ confirmed that the cytokine expressed in BHV-1-infected cells was biologically active. The effects of the recombinant IFN-γ were then analysed during a primary infection and after reactivation of a latent infection. During the primary infection, similar body temperature, clinical responses and virus shedding were observed for calves infected with either recombinant BHV-1/IFNγ or parental gC/LacZ virus. An analysis of cellular and humoral responses did not reveal any significant immunomodulation by BHV-1/IFNγ during the primary infection. The stability and activity of recombinant IFN-γ was also analysed following the establishment of a latent infection. The presence of recombinant IFN-γ did not significantly alter virus shedding following reactivation. The isolation of reactivated BHV-1/IFNγ virus confirmed that a functional IFN-γ gene was retained during latency. Thus, herpesviruses may provide virus vectors that retain functional genes during latency and recrudescence.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-11-2665
2000-11-01
2020-10-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/11/0812665a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-11-2665&mimeType=html&fmt=ahah

References

  1. Babiuk L. A., Bielefeldt Ohmann H., Gifford G., Czarniecki C. W., Scialli V. T., Hamilton E. B.. 1985; Effect of bovine α1 interferon on bovine herpesvirus type 1-induced respiratory disease. Journal of General Virology66:2383–2394
    [Google Scholar]
  2. Bembridge G. P., Lopez J. A., Cook R., Melero J. A., Taylor G.. 1998; Recombinant vaccinia virus coexpressing the F protein of respiratory syncytial virus (RSV) and interleukin-4 (IL-4) does not inhibit the development of RSV-specific memory cytotoxic T lymphocytes, whereas priming is diminished in the presence of high levels of IL-2 or gamma interferon. Journal of Virology72:4080–4087
    [Google Scholar]
  3. Bielefeldt Ohmann H., Babiuk L. A.. 1985; Viral–bacterial pneumonia in calves: effect of bovine herpesvirus-1 on immunologic functions. Journal of Infectious Diseases151:937–947
    [Google Scholar]
  4. Bielefeldt Ohmann H., Gilchrist J. E., Babiuk L. A.. 1984; Effect of recombinant DNA-produced bovine interferon alpha (BoIFN-α1) on the interaction between bovine alveolar macrophages and bovine herpesvirus type 1. Journal of General Virology65:1487–1495
    [Google Scholar]
  5. Bielefeldt Ohmann H., Lawman M. J., Babiuk L. A.. 1987; Bovine interferon: its biology and application in veterinary medicine. Antiviral Research7:187–210
    [Google Scholar]
  6. Boehm U., Klamp T., Groot M., Howard J. C.. 1997; Cellular responses to interferon-gamma. Annual Review of Immunology15:749–795
    [Google Scholar]
  7. Campos M., Ohmann H. B., Hutchings D., Rapin N., Babiuk L. A., Lawman M. J.. 1989; Role of interferon-gamma in inducing cytotoxicity of peripheral blood mononuclear leukocytes to bovine herpesvirus type 1 (BHV-1)-infected cells. Cell Immunology120:259–269
    [Google Scholar]
  8. Cerretti D. P., McKereghan K., Larsen A., Cosman D., Gillis S., Baker P. E.. 1986; Cloning, sequence and expression of bovine interferon-gamma. Journal of Immunology136:4561–4564
    [Google Scholar]
  9. Chiang Y. W., Roth J. A., Andrews J. J.. 1990; Influence of recombinant bovine interferon gamma and dexamethasone on pneumonia attributable to Haemophilus somnus in calves. American Journal of Veterinary Research51:759–762
    [Google Scholar]
  10. Denis M., Hanon E., Rijsewijk F. A., Kaashoek M. J., van Oirschot J. T., Thiry E., Pastoret P. P.. 1996; The role of glycoproteins gC, gE, gI, and gG in the induction of cell-mediated immune responses to bovine herpesvirus 1. Veterinary Microbiology53:121–132
    [Google Scholar]
  11. Fauci A. S., Pantaleo G., Stanley S., Weissman D.. 1996; Immunopathogenic mechanisms of HIV infection. Annals of Internal Medicine124:654–663
    [Google Scholar]
  12. Giavedoni L. D., Jones L., Gardner M. B., Gibson H. L., Ng C. T., Barr P. J., Yilma T.. 1992; Vaccinia virus recombinants expressing chimeric proteins of human immunodeficiency virus and gamma interferon are attenuated for nude mice. Proceedings of the National Academy of Sciences, USA89:3409–3413
    [Google Scholar]
  13. Giavedoni L., Ahmad S., Jones L., Yilma T.. 1997; Expression of gamma interferon by simian immunodeficiency virus increases attenuation and reduces postchallenge virus load in vaccinated rhesus macaques. Journal of Virology71:866–872
    [Google Scholar]
  14. Griebel P. J., Qualtiere L., Davis W. C., Lawman M. J., Babiuk L. A.. 1987; Bovine peripheral blood leukocyte subpopulation dynamics following a primary bovine herpesvirus-1 infection. Viral Immunology1:267–286
    [Google Scholar]
  15. Homan E. J., Easterday B. C.. 1983; Experimental latent and recrudescent bovine herpesvirus-1 infections in calves. American Journal of Veterinary Research44:309–313
    [Google Scholar]
  16. Karupiah G., Blanden R. V., Ramshaw I. A.. 1990; Interferon gamma is involved in the recovery of athymic nude mice from recombinant vaccinia virus/interleukin 2 infection. Journal of Experimental Medicine172:1495–1503
    [Google Scholar]
  17. Kohonen-Corish M. R., King N. J., Woodhams C. E., Ramshaw I. A.. 1990; Immunodeficient mice recover from infection with vaccinia virus expressing interferon-gamma. European Journal of Immunology20:157–161
    [Google Scholar]
  18. Kurzrock R., Rosenblum M. J., Sherwin S. A., Rios A., Talpaz M., Quesada J. R., Guttman J. U.. 1985; Pharmacokinetics, single-dose tolerance, and biological activity of recombinant gamma-interferon in cancer patients. Cancer Research45:2866–2872
    [Google Scholar]
  19. Leong K. H., Ramsay A. J., Boyle D. B., Ramshaw I. A.. 1994; Selective induction of immune responses by cytokines coexpressed in recombinant fowlpox virus. Journal of Virology68:8125–8130
    [Google Scholar]
  20. Liang X. P., Babiuk L. A., van Drunen Littel-van den Hurk S., Fitzpatrick D. R., Zamb T. J.. 1991a; Bovine herpesvirus 1 attachment to permissive cells is mediated by its major glycoproteins gI, gIII, and gIV. Journal of Virology65:1124–1132
    [Google Scholar]
  21. Liang X. P., Babiuk L. A., Zamb T. J.. 1991b; Pseudorabies virus gIII and bovine herpesvirus 1 gIII share complementary functions. Journal of Virology65:5553–5557
    [Google Scholar]
  22. Liang X., Babiuk L. A., Zamb T. J.. 1992; An in vivo study of a glycoprotein gIII-negative bovine herpesvirus 1 (BHV-1) mutant expressing beta-galactosidase: evaluation of the role of gIII in virus infectivity and its use as a vector for mucosal immunization. Virology189:629–639
    [Google Scholar]
  23. Muller U., Steinhoff U., Reis L. F., Hemmi S., Pavlovic J., Zinkernagel R. M., Aguet M.. 1994; Functional role of type I and type II interferons in antiviral defence. Science264:1918–1921
    [Google Scholar]
  24. Raggo C., Fitzpatrick D. R., Babiuk L. A., Liang X.. 1996; Expression of bovine interleukin-1 beta in a bovine herpesvirus-1 vector: in vitro analysis. Virology221:78–86
    [Google Scholar]
  25. Roth J. A., Frank D. E.. 1989; Recombinant bovine interferon-gamma as an immunomodulator in dexamethasone-treated and nontreated cattle. Journal of Interferon Research9:143–151
    [Google Scholar]
  26. Sambhi S. K., Kohonen-Corish M. R., Ramshaw I. A.. 1991; Local production of tumour necrosis factor encoded by recombinant vaccinia virus is effective in controlling viral replication in vivo. Proceedings of the National Academy of Sciences, USA88:4025–4029
    [Google Scholar]
  27. Straub C., Ahl R.. 1976; Local interferon production in cattle after intranasal infection with avirulent IBR/IPV virus and its effect on a subsequent infection with foot-and-mouth disease virus. Zentralblatt fuer Veterinaermedizin Beiheft/Reihe B23:470–482
    [Google Scholar]
  28. van Drunen Littel-van den Hurk S., van den Hurk J. V., Gilchrist J. E., Misra V., Babiuk L. A.. 1984; Interactions of monoclonal antibodies and bovine herpesvirus type 1 (BHV-1) glycoproteins: characterization of their biochemical and immunological properties. Virology135:466–479
    [Google Scholar]
  29. van Drunen Littel-van den Hurk S., Tikoo S. K., Liang X., Babiuk L. A.. 1993; Bovine herpesvirus-1 vaccines. Immunology and Cell Biology71:405–420
    [Google Scholar]
  30. van Oirschot J. T., Kaashoek M. J., Rijsewijk F. A.. 1996; Advances in the development and evaluation of bovine herpesvirus 1 vaccines. Veterinary Microbiology53:43–54
    [Google Scholar]
  31. Wood G. S.. 1990; The immunohistology of lymph nodes in HIV infection: a review. Progress in AIDS Pathology2:25–32
    [Google Scholar]
  32. Wyler R., Engels M., Schwyzer M.. 1989; Infectious bovine rhinotracheitis/vulvovaginitis (BHV-1). In Herpesvirus Diseases of Cattle Horses and Pigs pp1–72 Edited by Wittman G.. Boston: Kluwer;
    [Google Scholar]
  33. Yates W. D.. 1982; A review of infectious bovine rhinotracheitis, shipping fever pneumonia and viral–bacterial synergism in respiratory disease of cattle. Canadian Journal of Comparative Medicine46:225–263
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-11-2665
Loading
/content/journal/jgv/10.1099/0022-1317-81-11-2665
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error