1887

Abstract

The herpesvirus saimiri (HVS) gene product encoded by ORF73 shares a limited homology with the ORF73 encoded protein of Kaposi’s sarcoma-associated herpesvirus (KSHV). It has recently been shown that the KSHV ORF73 protein is expressed during a latent infection and co-localizes with host cell chromosomes, suggesting that it plays a role in episomal maintenance by tethering viral genomes to host cell chromosomes. At present the role of the HVS ORF73 gene product is unknown. However, the expression of HVS ORF73 in a stably transduced human carcinoma cell line, where the HVS genome persists as a non-integrated circular episome, has recently been shown. In this report, the characterization of the HVS ORF73 protein and the mapping of its functional domains are described. The results suggest that the HVS ORF73 gene encodes a 64 kDa nuclear protein. Moreover, the amino terminus contains two functional nuclear localization signals, whereas the carboxy terminus is required for the distinctive speckled nuclear distribution pattern as observed with both the HVS and KSHV ORF73 proteins.

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2000-11-01
2024-12-03
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References

  1. Albrecht J. C., Nicholas J., Biller D., Cameron K. R., Biesinger B., Newman C., Wittmann S., Craxton M. A., Coleman H., Fleckenstein B., Honess R. W. 1992; Primary structure of the herpesvirus saimiri genome. Journal of Virology 66:5047–5058
    [Google Scholar]
  2. Ballestas M. E., Chatis P. A., Kaye K. M. 1999; Efficient persistence of extrachromosomal KSHV DNA mediated by latency-associated nuclear antigen. Science 284:641–644
    [Google Scholar]
  3. Biesinger B., Muller-Fleckenstein I., Simmer B., Lang G., Wittmann S., Platzer E., Desrosiers R. C., Fleckenstein B. 1992; Stable growth transformation of human T lymphocytes by herpesvirus saimiri. Proceedings of the National Academy of Sciences, USA 89:3116–3119
    [Google Scholar]
  4. Chang Y., Moore P. S., Talbot S. J., Boshoff C. H., Zarkowska T., Godden K., Paterson H., Weiss R. A., Mittnacht S. 1996; Cyclin encoded by KS herpesvirus. Nature 382:410
    [Google Scholar]
  5. Cotter M. A., Robertson E. S. 1999; The latency-associated nuclear antigen tethers the Kaposi’s sarcoma-associated herpesvirus genome to host chromosomes in body cavity-based lymphoma cells. Virology 264:254–264
    [Google Scholar]
  6. Dittmer D., Lagunoff M., Renne R., Staskus K., Haase A., Ganem D. 1998; A cluster of latently expressed genes in Kaposi’s sarcoma-associated herpesvirus. Journal of Virology 72:8309–8315
    [Google Scholar]
  7. Fleckenstein B., Desrosiers R. C. 1982; Herpesvirus saimiri and herpesvirus ateles. In The Herpesviruses pp 253–332 Edited by Roizman B. New York: Plenum Press;
    [Google Scholar]
  8. Friborg J., Kong W. P., Hottinger M. O., Nabel G. J. 1999; p53 inhibition by the LANA protein of KSHV protects against cell death. Nature 402:889–894
    [Google Scholar]
  9. Hall K. T., Giles M. S., Goodwin D. J., Calderwood M. A., Carr I. M., Stevenson A. J., Markham A. F., Whitehouse A. 2000; Analysis of gene expression in a human cell line stably transduced with herpesvirus saimiri. Journal of Virology 74:7331–7337
    [Google Scholar]
  10. Jung J. U., Stager M., Desrosiers R. C. 1994; Virus-encoded cyclin. Molecular and Cellular Biology 14:7235–7244
    [Google Scholar]
  11. Kedes D. H., Lagunoff M., Renne R., Ganem D. 1997; Identification of the gene encoding the major latency-associated nuclear antigen of the Kaposi’s sarcoma-associated herpesvirus. Journal of Clinical Investigation 100:2606–2610
    [Google Scholar]
  12. Kellam P., Boshoff C., Whitby D., Matthews S., Weiss R. A., Talbot S. J. 1997; Identification of a major latent nuclear antigen LNA-1, in the human herpesvirus 8 genome. Journal of Human Virology 1:19–29
    [Google Scholar]
  13. Lomonte P., Bublot M., van Santen V., Keil G. M., Pastoret P.-P., Thiry E. 1995; Analysis of bovine herpesvirus 4 genomic regions located outside the conserved gammaherpesvirus gene blocks. Journal of General Virology 76:1835–1841
    [Google Scholar]
  14. Neipel F., Albrecht J. C., Fleckenstein B. 1997; Cell-homologous genes in the Kaposi’s sarcoma-associated rhadinovirus human herpesvirus 8: determinants of its pathogenicity?Journal of. Virology 71:4187–4192
    [Google Scholar]
  15. Platt G. M., Simpson G., Mittnacht S., Schulz T. 1999; Latent nuclear antigen of Kaposi’s sarcoma-associated herpesvirus interacts with RING3, a homologue of the Drosophila female sterile homeotic (fsh) gene. Journal of Virology 73:9789–9795
    [Google Scholar]
  16. Rainbow L., Platt G. M., Simpson G. R., Sarid R., Gao S.-J., Stoiber H., Herrington C. S., Moore P. S., Schulz T. F. 1997; The 222- to 234-kilodalton latent nuclear protein (LNA) of Kaposi’s sarcoma-associated herpesvirus (human herpesvirus 8) is encoded by ORF73 and is a component of the latency-associated nuclear antigen. Journal of Virology 71:5915–5921
    [Google Scholar]
  17. Russo J. J., Bohenzky R. A., Chien M. C., Chen J., Yan M., Maddalena D., Parry J. P., Peruzzi D., Edelman I. S., Chang Y., Moore P. S. 1996; Nucleotide sequence of the Kaposi’s sarcoma-associated herpesvirus (HHV8). Proceedings of the National Academy of Sciences, USA 93:14862–14867
    [Google Scholar]
  18. Sarid R., Flore O., Bohenzky R. A., Chang Y., Moore P. S. 1998; Transcription mapping of the Kaposi’s sarcoma-associated herpesvirus (human herpesvirus 8) genome in a body cavity-based lymphoma cell line (BC-1). Journal of Virology 72:1005–1012
    [Google Scholar]
  19. Talbot S. J., Weiss R. A., Kellam P., Boshoff C. 1999; Transcriptional analysis of human herpesvirus 8 open reading frames 71, 72, 73, K14 and 74 in a primary effusion lymphoma cell line. Virology 257:84–94
    [Google Scholar]
  20. Telford E. A. R., Watson M. S., Aird H. C., Perry J., Davison A. J. 1995; The DNA sequence of equine herpesvirus 2. Journal of Molecular Biology 249:520–528
    [Google Scholar]
  21. Thome M., Schneider P., Hofmann K., Fickenscher H., Meinl E., Neipel F., Mattmann C., Burns K., Bodmer J. L., Schroter M., Scaffidi C., Krammer P. H., Peter M. E., Tschopp J. 1997; Viral FLICE-inhibitory protein (FLIPs) prevent apoptosis induced by death receptors. Nature 386:517–521
    [Google Scholar]
  22. Virgin H. W.IV., Latreille P., Wamsley P., Hallsworth K., Weck K. E., Dal Canto A. J., Speck S. H. 1997; Complete sequence and genomic analysis of murine gammaherpesvirus 68. Journal of Virology 71:5894–5904
    [Google Scholar]
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