1887

Abstract

Mice (ddY strain, 4 weeks old) were infected intranasally with the H5N1 influenza viruses A/Hong Kong/156/97 (HK156) and A/Hong Kong/483/97 (HK483) isolated from humans. HK156 and HK483 required 200 and 5 p.f.u. of virus, respectively, to give a 50% lethal dose to the mice when the volume of inoculum was set at 10 μl. Both viruses caused encephalitis and severe bronchopneumonia in infected mice. The severity of lung lesions caused by the viruses was essentially similar, whereas HK483 caused more extensive lesions in the brain than did HK156. This was supported by the results of virus titration of organ homogenates, which showed that the virus titres in brains of HK483-infected mice were more than 100-fold higher than those of HK156-infected mice, while those in lungs were almost equivalent. Both viruses were detected in homogenates of the heart, liver, spleen and kidney and blood of the infected mice. Virus antigen was detected by immunohistology in the heart and liver, albeit sporadically, but caused no degenerative change in these organs. The antigen was not detected in the thymus, spleen, pancreas, kidney or gastrointestinal tract. In contrast, virus antigen was found frequently in adipose tissues attached to those organs. The adipose tissues showed severe degenerative change and the virus titres in the tissues were high and comparable to those in lungs. Thus, infection of HK156 and HK483 in our mouse model was pneumo-, neuro- and adipotropic, but not pantropic. Furthermore, HK483 showed higher neurotropism than HK156, which may account for its higher lethality.

Erratum

This article contains a correction applying to the following content:
Characterization of human influenza A (H5N1) virus infection in mice: neuro-, pneumo- and adipotropic infection
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-81-10-2503
2000-10-01
2020-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/81/10/0812503a.html?itemId=/content/journal/jgv/10.1099/0022-1317-81-10-2503&mimeType=html&fmt=ahah

References

  1. Baer G. M.. 1975; Rabies in nonhematophagous bats. In The Natural History of Rabies pp79–97 Edited by Baer G. M.. New York, San Francisco & London: Academic Press;
    [Google Scholar]
  2. Bender C., Hall H., Huang J., Klimov A., Cox N., Hay A., Gregory V., Cameron K., Lim W., Subbarao K.. 1999; Characteristics of the surface proteins of influenza A (H5N1) viruses isolated from humans in 1997–1998. Virology254:115–123
    [Google Scholar]
  3. Botvinkin A. D., Nikiforova T. A., Sidorov G. N.. 1985; Experimental rabies in hibernator rodents. Acta Virologica29:44–50
    [Google Scholar]
  4. Bruggeman C. A., Bruning J. H., Grauls G., van den Bogaard A. E. J. M., Bosman F.. 1987; Presence of cytomegalovirus in brown fat. Study in a rat model. Intervirology27:32–37
    [Google Scholar]
  5. Claas E. C., Osterhaus A. D., van Beek R., De Jong J. C., Rimmelzwaan G. F., Senne D. A., Krauss S., Shortridge K. F., Webster R. G.. 1998; Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus. Lancet351:472–477
    [Google Scholar]
  6. Dybing J. K., Schultz-Cherry S., Swayne D. E., Suarez D. L., Perdue M. L.. 2000; Distinct pathogenesis of Hong Kong-origin H5N1 viruses in mice compared to that of other highly pathogenic H5 avian influenza viruses. Journal of Virology74:1443–1450
    [Google Scholar]
  7. Gao P., Watanabe S., Ito T., Goto H., Wells K., McGregor M., Cooley A. J., Kawaoka Y.. 1999; Biological heterogeneity, including systemic replication in mice, of H5N1 influenza A virus isolates from humans in Hong Kong. Journal of Virology73:3184–3189
    [Google Scholar]
  8. Gubareva L. V., McCullers J. A., Bethell R. C., Webster R. G.. 1998; Characterization of influenza A/Hong Kong/156/97 (H5N1) virus in a mouse model and protective effect of zanamivir on H5N1 infection in mice. Journal of Infectious Diseases178:1592–1596
    [Google Scholar]
  9. Hashimoto I., Tomita M., Kohara T., Uchino I., Harashima A.. 1985; Steatitis in mice infected with coxsackie virus B3. Microbiology and Immunology29:371–376
    [Google Scholar]
  10. Hinshaw V.. 1998; Influenza in other species (seal, whale and mink). In Textbook of Influenza pp163–167 Edited by Nicholson K., Webster R. G., Hay A.. London: Blackwell Science;
    [Google Scholar]
  11. Iwasaki T., Tamura S., Kumasaka T., Sato Y., Hasegawa H., Asanuma H., Aizawa S., Yanagihara R., Kurata T.. 1999; Exacerbation of influenza virus pneumonia by intranasal administration of surfactant in a mouse model. Archives of Virology144:675–685
    [Google Scholar]
  12. Kurata T., Tsai T. F., Bauer S. P., McCormick J. B.. 1983; Immunofluorescence studies of disseminated Hantaan virus infection of suckling mice. Infection and Immunity41:391–398
    [Google Scholar]
  13. Kurtz J., Manvell R. J., Banks J.. 1996; Avian influenza virus isolated from a woman with conjunctivitis. Lancet348:901–902
    [Google Scholar]
  14. Li S., Liu C., Klimov A., Subbarao K., Perdue M. L., Mo D., Ji Y., Woods L., Hietala S., Bryant M.. 1999; Recombinant influenza A virus vaccines for the pathogenic human A/Hong Kong/97 (H5N1) viruses. Journal of Infectious Diseases179:1132–1138
    [Google Scholar]
  15. Lu X., Tumpey T. M., Morken T., Zaki S. R., Cox N. J., Katz J. M.. 1999; A mouse model for the evaluation of pathogenesis and immunity to influenza A (H5N1) viruses isolated from humans. Journal of Virology73:5903–5911
    [Google Scholar]
  16. Murphy F. A., Taylor W. P., Mims C. A., Marshall I. D.. 1973; Pathogenesis of Ross River virus infection in mice. II. Muscle, heart, and brown fat lesions. Journal of Infectious Diseases127:129–138
    [Google Scholar]
  17. Price P., Eddy K. S., Papadimitriou J. M., Robertson T. A., Shellam G. R.. 1990; Cytomegalovirus infection of adipose tissues induces steatitis in adult mice. International Journal of Experimental Pathology71:557–571
    [Google Scholar]
  18. Shortridge K. F., Zhou N. N., Guan Y., Gao P., Ito T., Kawaoka Y., Kodihalli S., Krauss S., Markwell D., Murti K. G., Norwood M., Senne D., Sims L., Takada A., Webster R. G.. 1998; Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virology252:331–342
    [Google Scholar]
  19. Suarez D. L., Perdue M. L., Cox N., Rowe T., Bender C., Huang J., Swayne D. E.. 1998; Comparisons of highly virulent H5N1 influenza A viruses isolated from humans and chickens from Hong Kong. Journal of Virology72:6678–6688
    [Google Scholar]
  20. Subbarao K., Klimov A., Katz J., Regnery H., Lim W., Hall H., Perdue M., Swayne D., Bender C., Huang J., Hemphill M., Rowe T., Shaw M., Xu X., Fukuda K., Cox N.. 1998; Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness. Science279:393–396
    [Google Scholar]
  21. Takada A., Kuboki N., Okazaki K., Ninomiya A., Tanaka H., Osaki H., Itamura S., Nishimura H., Enami M., Tashiro M., Shortridge K. F., Kida H.. 1999; Avirulent avian influenza virus as a vaccine strain against a potential human pandemic. Journal of Virology73:8303–8307
    [Google Scholar]
  22. Webster R. G., Geraci J., Petursson G., Skirnisson K.. 1981; Conjunctivitis in human beings caused by influenza A virus of seals. New England Journal of Medicine304:911
    [Google Scholar]
  23. Yang H. Y., Joris I., Majno G., Welsh R. M.. 1985; Necrosis of adipose tissue induced by sequential infections with unrelated viruses. American Journal of Pathology120:173–177
    [Google Scholar]
  24. Yuen K. Y., Chan P. K. S., Peiris M., Tsang D. N. C., Que T. L., Shortridge K. F., Cheung P. T., To W. K., Ho E. T. F., Sung R., Cheng A. F. B.. and members of the H5N1 study group. 1998; Clinical features and rapid viral diagnosis of human disease associated with avian influenza A H5N1 virus. Lancet351:467–471
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-81-10-2503
Loading
/content/journal/jgv/10.1099/0022-1317-81-10-2503
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error