1887

Abstract

A clonal population of BHK-21 cells resistant to infection with the low-neurovirulence BeAn strain of Theiler’s murine encephalomyelitis virus (TMEV) was derived after four cycles of infection and characterized. These cells were resistant to both low- and high-neurovirulence TMEV strains due to a block in virus attachment and entry and not in virus replication, since transfection of these cells with TMEV RNA to bypass the entry step(s) induced virus replication and assembly. The resistance to infection was stable for more than a year, suggesting that it is a heritable property arising from a mutation in the susceptible parent BHK-21 population. This cell line is being used to identify a receptor for TMEV.

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/content/journal/jgv/10.1099/0022-1317-81-10-2485
2000-10-01
2020-01-29
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References

  1. Blakemore, W. F., Welsh, C. J., Tonks, P. & Nash, A. A. ( 1988; ). Observations on demyelinating lesions induced by Theiler’s virus in CBA mice. Acta Neuropathologica 76, 581-589.[CrossRef]
    [Google Scholar]
  2. Borzakian, S., Couderc, T., Barbier, Y., Attal, G., Pelletier, I. & Colbere-Garapin, F. ( 1992; ). Persistent poliovirus infection: establishment and maintenance involve distinct mechanisms. Virology 186, 398-408.[CrossRef]
    [Google Scholar]
  3. Casals, J. ( 1963; ). Immunological characterization of Vilyuisk human encephalomyelitis virus. Nature 200, 339-341.[CrossRef]
    [Google Scholar]
  4. Clatch, R. J., Lipton, H. L. & Miller, S. D. ( 1986; ). Characterization of Theiler’s murine encephalomyelitis virus (TMEV)-specific delayed-type hypersensitivity responses in TMEV-induced demyelinating disease: correlation with clinical signs. Journal of Immunology 136, 920-926.
    [Google Scholar]
  5. Fotiadis, C., Kilpatrick, D. R. & Lipton, H. L. ( 1991; ). Comparison of the binding characteristics to BHK-21 cells of viruses representing the two Theiler’s virus neurovirulence groups. Virology 182, 365-370.[CrossRef]
    [Google Scholar]
  6. Gerety, S. J., Rundell, K. M., Dal Canto, M. C. & Miller, S. D. ( 1994; ). Class II-restricted T cell responses in Theiler’s murine encephalomyelitis virus-induced demyelinating disease. VI. Potentiation of demyelination with and characterization of an immunopathologic CD4+ T cell line specific for an immunodominant VP2 epitope. Journal of Immunology 152, 919-929.
    [Google Scholar]
  7. Hara, T., Hattori, S. & Kawakita, M. ( 1989; ). Isolation and characterization of mouse FM3A cell mutants which are devoid of Newcastle disease receptors. Journal of Virology 63, 182-183.
    [Google Scholar]
  8. Hertzler, S., Luo, M. & Lipton, H. L. ( 2000; ). Mutation of predicted virion pit residues alters binding of Theiler’s murine encephalomyelitis virus to BHK-21 cells. Journal of Virology 74, 1994-2004.[CrossRef]
    [Google Scholar]
  9. Ishida, N., Miura, N., Yoshioka, S. & Kawakita, M. ( 1996; ). Molecular cloning and characterization of a novel isoform of the human UDP-galactose transporter, and of related complementary DNAs belonging to the nucleotide sugar transporter gene family. Journal of Biochemistry 120, 1074-1078.[CrossRef]
    [Google Scholar]
  10. Jnaoui, K. & Michiels, T. ( 1999; ). Analysis of cellular mutants resistant to Theiler’s virus infection: differential infection of L929 cells by persistent and neurovirulent strains. Journal of Virology 73, 7248-7254.
    [Google Scholar]
  11. Kaplan, G. & Racaniello, V. R. ( 1991; ). Down regulation of poliovirus receptor RNA in HeLa cells resistant to poliovirus infection. Journal of Virology 65, 1829-1835.
    [Google Scholar]
  12. Kaplan, G., Levy, A. & Racaniello, V. R. ( 1989; ). Isolation and characterization of HeLa cell lines blocked at different steps in the poliovirus life cycle. Journal of Virology 63, 43-51.
    [Google Scholar]
  13. Rodriguez, M., Leibowitz, J. L. & Lampert, P. W. ( 1983; ). Persistent infection of oligodendrocytes in Theiler’s virus-induced encephalomyelitis. Annals of Neurology 13, 426-433.[CrossRef]
    [Google Scholar]
  14. Rozhon, E. J., Kratochvil, J. D. & Lipton, H. L. ( 1983; ). Analysis of genetic variation in Theiler’s virus during persistent infection in the mouse central nervous system. Virology 128, 16-32.[CrossRef]
    [Google Scholar]
  15. Yoshioka, S., Sun-Wada, G.-H., Ishida, N. & Kawakita, M. ( 1997; ). Expression of human UDP-galactose transporter in the Golgi membranes of murine Had-1 cells that lack the endogenous transporter. Journal of Biochemistry 122, 691-695.[CrossRef]
    [Google Scholar]
  16. Zhou, L., Lin, X., Green, T. J., Lipton, H. L. & Luo, M. ( 1997; ). Role of sialyloligosaccharide binding in Theiler’s virus persistence. Journal of Virology 71, 9701-9712.
    [Google Scholar]
  17. Zhou, L., Luo, Y., Wu, Y., Tsao, J. & Luo, M. ( 2000; ). Sialylation of the host receptor may modulate entry demyelinating persistent Theiler’s virus. Journal of Virology 74, 1477-1485.[CrossRef]
    [Google Scholar]
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