1887

Abstract

Human papillomaviruses (HPVs) cause a variety of clinical manifestations, including the most prevalent viral sexually transmitted disease, genital warts. HPV-6 is found in a greater number of genital warts than any other HPV. To increase our understanding of the structural and functional relationships between HPV-6 isolates and to provide information for epidemiological studies, the sequences of the E2, E6 and E7 coding regions of HPV-6 genomes in clinical samples were determined. This sequence analysis was performed on isolates originally designated HPV-6a on the basis of analysis of patterns generated by restriction enzyme digestion. It was found that the designation of subtype on the basis of restriction enzyme digestion correlated poorly with the designation of subtype on the basis of sequence comparison; in fact, the clinical isolates were clearly categorized into HPV-6a and HPV-6b groups, with the previously described HPV-6vc being a member of the HPV-6a group. It was also found that the HPV-6a E2 protein is a much less potent activator of transcription than the HPV-16 E2 protein, generalizing our previous results with the HPV-6b E2 protein to this second HPV-6 E2 protein. These studies indicate that the amino acid differences observed between these natural variants of the HPV-6 E2 protein do not affect its function.

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/content/journal/jgv/10.1099/0022-1317-80-9-2445
1999-09-01
2024-12-12
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References

  1. Bernard B. A., Bailly C., Lenoir M. C., Darmon M., Thierry F., Yaniv M. 1989; The human papillomavirus type 18 (HPV18) E2 gene product is a repressor of the HPV18 regulatory region in human keratinocytes. Journal of Virology 63:4317–4324
    [Google Scholar]
  2. Boshart M., zur Hausen H. 1986; Human papillomaviruses in Buschke-Löwenstein tumors: physical state of the DNA and identification of a tandem duplication in the noncoding region of a human papillomavirus 6 subtype. Journal of Virology 58:963–966
    [Google Scholar]
  3. Chan S. Y., Bernard H. U., Ong C. K., Chan S. P., Hofmann B., Delius H. 1992; Phylogenetic analysis of 48 papillomavirus types and 28 subtypes and variants: a showcase for the molecular evolution of DNA viruses. Journal of Virology 66:5714–5725
    [Google Scholar]
  4. Chin M. T., Hirochika R., Hirochika H., Broker T. R., Chow L. T. 1988; Regulation of human papillomavirus type 11 enhancer and E6 promoter by activating and repressing proteins from the E2 open reading frame: functional and biochemical studies. Journal of Virology 62:2994–3002
    [Google Scholar]
  5. Dostatni N., Lambert P. F., Sousa R., Ham J., Howley P. M., Yaniv M. 1991; The functional BPV-1 E2 trans-activating protein can act as a repressor by preventing formation of the initiation complex. Genes & Development 5:1657–1671
    [Google Scholar]
  6. Farr A., Wang H., Kasher M. S., Roman A. 1991; Relative enhancer activity and transforming potential of authentic human papillomavirus type 6 genomes from benign and malignant lesions. Journal of General Virology 72:519–526
    [Google Scholar]
  7. Gissmann L., Wolnik L., Ikenberg H., Koldovsky U., Schnurch H. G., zur Hausen H. 1983; Human papillomavirus types 6 and 11 DNA sequences in genital and laryngeal papillomas and in some cervical cancers. Proceeding of the National Academy of Sciences, USA 80:560–563
    [Google Scholar]
  8. Grassmann K., Wilczynski S. P., Cook N., Rapp B., Iftner T. 1996; HPV6 variants from malignant tumors with sequence alterations in the regulatory region do not reveal differences in the activities of the oncogene promoters but do contain amino acid exchanges in the E6 and E7 proteins. Virology 223:185–197
    [Google Scholar]
  9. Heinzel P. A., Chan S. Y., Ho L., O’Connor M., Balaram P., Campo M. S., Fujinaga K., Kiviat N., Kuypers J., Pfister H., Steinberg B. M., Tay S. K., Villa L. L., Bernard H. U. 1995; Variation of human papillomavirus type 6 (HPV-6) and HPV-11 genomes sampled throughout the world. Journal of Clinical Microbiology 33:1746–1754
    [Google Scholar]
  10. Hofmann K. J., Cook J. C., Joyce J. G., Brown D. R., Schultz L. D., George H. A., Rosolowsky M., Fife K. H., Jansen K. U. 1995; Sequence determination of human papillomavirus type 6a and assembly of virus-like particles in Saccharomyces cerevisiae. Virology 209:506–518
    [Google Scholar]
  11. Icenogle J. P., Sathya P., Miller D. L., Tucker R. A., Rawls W. E. 1991; Nucleotide and amino acid sequence variation in the L1 and E7 open reading frames of human papillomavirus type 6 and type 16. Virology 184:101–107
    [Google Scholar]
  12. Kasher M. S., Roman A. 1988; Characterization of human papillomavirus type 6b DNA isolated from an invasive squamous carcinoma of the vulva. Virology 165:225–233
    [Google Scholar]
  13. Kitasato H., Delius H., zur Hausen H., Sorger K., Rösl F., de Villiers E.-M. 1994; Sequence rearrangements in the upstream regulatory region of human papillomavirus type 6: are these involved in malignant transition?. Journal of General Virology 75:1157–1162
    [Google Scholar]
  14. Kovelman R., Bilter G. K., Glezer E., Tsou A. Y., Barbosa M. S. 1996; Enhanced transcriptional activation by E2 proteins from the oncogenic human papillomaviruses. Journal of Virology 70:7549–7560
    [Google Scholar]
  15. Krige D., Mills H. R., Berrie E. L., Doherty N. C., Jones D. K., Ryan C. A., Davies H., Myint S., McCance D. J., Layton G. T., French T. J. 1997; Sequence variation in the early genes E1E4, E6 and E7 of human papilloma virus type 6. Virus Research 49:187–191
    [Google Scholar]
  16. Oft M., Bohm S., Wilczynski S. P., Iftner T. 1993; Expression of the different viral mRNAs of human papilloma virus 6 in a squamous-cell carcinoma of the bladder and the cervix. International Journal of Cancer 53:924–931
    [Google Scholar]
  17. Rando R. F., Groff D. E., Chirikjian J. G., Lancaster W. D. 1986; Isolation and characterization of a novel human papillomavirus type 6 DNA from an invasive vulvar carcinoma. Journal of Virology 57:353–356
    [Google Scholar]
  18. Roman A., Brown D. 1995; Sequence variation in the extreme 5′ end of the human papillomavirus type 6a long control region. Journal of Infectious Diseases 171:697–700
    [Google Scholar]
  19. Rübben A., Beaudenon S., Favre M., Schmitz W., Spelten B., Grussendorf-Conen E.-I. 1992; Rearrangements of the upstream regulatory region of human papillomavirus type 6 can be found in both Buschke-Löwenstein tumours and in condylomata acuminata. Journal of General Virology 73:3147–3153
    [Google Scholar]
  20. Schwarz E., Dürst M., Demankowski C., Lattermann O., Zech R., Wolfsperger E., Suhai S., zur Hausen H. 1983; DNA sequence and genome organization of genital human papillomavirus type 6b. EMBO Journal 2:2341–2348
    [Google Scholar]
  21. Thierry F., Yaniv M. 1987; The BPV1-E2 trans-acting protein can be either an activator or a repressor of the HPV18 regulatory region. EMBO Journal 6:3391–3397
    [Google Scholar]
  22. Wilczynski S. P., Oft M., Cook N., Liao S. Y., Iftner T. 1993; Human papillomavirus type 6 in squamous cell carcinoma of the bladder and cervix. Human Pathology 24:96–102
    [Google Scholar]
  23. Yaegashi N., Xi L., Batra M., Galloway D. A. 1993; Sequence and antigenic diversity in two immunodominant regions of the L2 protein of human papillomavirus types 6 and 16. Journal of Infectious Diseases 168:743–747
    [Google Scholar]
  24. zur Hausen H. 1991; Human papillomaviruses in the pathogenesis of anogenital cancer. Virology 184:9–13
    [Google Scholar]
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