1887

Abstract

Studies on Marek’s disease virus serotype 2 (MDV2) are important for understanding the natural nononcogenic phenotype of MDV. This study reports a 27535 bp nucleotide sequence of part of the MDV2 genome located in the central unique long (U) region. The analysis revealed 11 complete ORFs with high amino acid sequence identities to the products of other alphaherpesviruses. The MDV2 ORFs were arranged collinearly with the prototype sequence of herpes simplex virus type 1, ranging from the UL30 to UL40 genes. Sequences that were particularly well conserved among alphaherpesviruses were the putative functional domain of the DNA polymerase (UL30) and the ribonucleotide reductase large and small subunits (UL39 and UL40). On the other hand, in contrast to oncogenic MDV1, MDV2 did not contain the conserved proline-repeat region in the UL36 homologue. All the genes identified were confirmed to be transcribed as 3′-coterminal mRNAs and/or unique transcripts in virus-infected cells.

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1999-09-01
2020-01-21
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References

  1. Chang, Y. E. & Roizman, B. ( 1993; ). The product of the UL31 gene of herpes simplex virus 1 is a nuclear phosphoprotein which partitions with the nuclear matrix. Journal of Virology 67, 6348-6356.
    [Google Scholar]
  2. Chang, Y. E., Poon, A. P. W. & Roizman, B. ( 1996; ). Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1. (1996). Journal of Virology 70, 3938-3946.
    [Google Scholar]
  3. Chodosh, L. A., Baldwin, A. S., Carthew, R. W. & Sharp, P. A. ( 1988; ). Human CCAAT-binding proteins have heterologous subunits. Cell 53, 11-24.[CrossRef]
    [Google Scholar]
  4. Davison, A. J. & Scott, J. E. ( 1986; ). The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67, 1759-1816.[CrossRef]
    [Google Scholar]
  5. Dutia, B. M., Frame, M. C., Subak-Sharpe, J. H., Clark, W. N. & Marsden, H. S. ( 1986; ). Specific inhibition of herpesvirus ribonucleotide reductase by synthetic peptides. Nature 321, 439-441.[CrossRef]
    [Google Scholar]
  6. Fuchs, W. & Mettenleiter, T. C. ( 1996; ). DNA sequence and transcriptional analysis of the UL1 to UL5 gene cluster of infectious laryngotracheitis virus. Journal of General Virology 77, 2221-2229.[CrossRef]
    [Google Scholar]
  7. Izumiya, Y., Jang, H.-K., Kashiwase, H., Cai, J.-S., Nishimura, Y., Tsushima, Y., Kato, K., Miyazawa, T., Kai, C. & Mikami, T. ( 1998; ). Identification and transcriptional analysis of the homologues of the herpes simplex virus type 1 UL41 to UL51 genes in the genome of nononcogenic Marek’s disease virus type 2. Journal of General Virology 79, 1997-2001.
    [Google Scholar]
  8. Jang, H.-K., Ono, M., Kim, T.-J., Izumiya, Y., Damiani, A. M., Matsumura, T., Niikura, M., Kai, C. & Mikami, T. ( 1998; ). The genetic organization and transcriptional analysis of the short unique region in the genome of nononcogenic Marek’s disease virus serotype 2. Virus Research 58, 137-147.[CrossRef]
    [Google Scholar]
  9. Lee, L. F., Wu, P. & Sui, D. ( 1996; ). Identification and transcriptional analysis of a Marek’s disease virus gene encoding membrane glycoprotein gp82. In Current Research on Marek’s Disease, pp. 245-250. Edited by R. F. Silva, H. H. Cheng, P. M. Coussens, L. F. Lee & L. F. Velicer. Kennett Square, PA: American Association of Avian Pathologists.
  10. Lin, J. A., Kitagawa, H., Ono, M., Iwanaga, R., Kodama, H. & Mikami, T. ( 1990; ). Isolation of serotype 2 Marek’s disease virus from birds belonging to genus Gallus in Japan. Avian Diseases 34, 336-344.[CrossRef]
    [Google Scholar]
  11. Liuzzi, M., Déziel, R., Moss, N., Beaulieu, P., Bonneau, A.-M., Bousquet, C., Chafouleas, J. G., Garneau, M., Jaramillo, J., Krogsrud, R. L., Lagacé, L., McCollum, R. S., Nawoot, S. & Guindon, Y. ( 1994; ). A potent peptidomimetic inhibitor of HSV ribonucleotide reductase with antiviral activity in vivo. Nature 372, 695-698.[CrossRef]
    [Google Scholar]
  12. McGeoch, D. J., Dalrymple, M. A., Davison, A. J., Dolan, A., Frame, M. C., McNab, D., Perry, L. J., Scott, J. E. & Taylor, P. ( 1988; ). The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69, 1531-1574.[CrossRef]
    [Google Scholar]
  13. McKnight, S. L., Kingsbury, R. C., Spence, A. & Smith, M. ( 1984; ). The distal transcription signals of the herpesvirus tk gene share a common hexanucleotide control sequence. Cell 37, 253-262.[CrossRef]
    [Google Scholar]
  14. Mao, Y., Wu, P., Kung, H.-J. & Lee, L. F. ( 1996; ). DNA sequence analysis of MDV genomic fragments BamHI-P2, -I1, -J and EcoRI-E reveals a large open reading frame homologous to a herpes simplex virus gene encoding a DNA-binding tegument protein. In Current Research on Marek’s Disease, pp. 182-188. Edited by R. F. Silva, H. H. Cheng, P. M. Coussens, L. F. Lee & L. F. Velicer. Kennett Square, PA: American Association of Avian Pathologists.
  15. Nikas, I., McLauchlan, J., Davison, A. J., Taylor, W. R. & Clements, J. B. ( 1986; ). Structural features of ribonucleotide reductase. Proteins 1, 376-384.[CrossRef]
    [Google Scholar]
  16. Ono, M., Katsuragi-Iwanaga, R., Kitazawa, T., Kamiya, N., Horimoto, T., Niikura, M., Kai, C., Hirai, K. & Mikami, T. ( 1992; ). The restriction endonuclease map of Marek’s disease virus (MDV) serotype 2 and collinear relationship among three serotypes of MDV. Virology 191, 459-463.[CrossRef]
    [Google Scholar]
  17. Purves, F. C., Spector, D. & Roizman, B. ( 1991; ). The herpes simplex virus 1 protein kinase encoded by the US3 gene mediates posttranslational modification of the phosphoprotein encoded by the UL34 gene. Journal of Virology 65, 5757-5764.
    [Google Scholar]
  18. Riggio, M. P. & Onions, D. E. ( 1994; ). Sequence of the ribonucleotide reductase-encoding genes of equine herpesvirus 4. Gene 143, 217-222.[CrossRef]
    [Google Scholar]
  19. Roizman, B. & Sears, A. E. ( 1996; ). Herpes simplex viruses and their replication. In Fields Virology, pp. 2231-2295. Edited by B. N. Field, D. M. Knipe & P. M. Howley. Philadelphia: Lippincott–Raven.
  20. Schwyzer, M. & Ackermann, M. ( 1996; ). Molecular virology of ruminant herpesviruses. Veterinary Microbiology 53, 17-29.[CrossRef]
    [Google Scholar]
  21. Sui, D., Wu, P., Kung, H.-J. & Lee, L. F. ( 1995; ). Identification and characterization of a Marek’s disease virus gene encoding DNA polymerase. Virus Research 36, 269-278.[CrossRef]
    [Google Scholar]
  22. Telford, E. A. R., Watson, M. S., McBride, K. & Davison, A. J. ( 1992; ). The DNA sequence of equine herpesvirus-1. Virology 189, 304-316.[CrossRef]
    [Google Scholar]
  23. Wu, P., Sui, D. & Lee, L. F. ( 1996; ). Nucleotide sequence analysis of a 9-kb region of Marek’s disease virus genome exhibits a collinear gene arrangement with the UL29 to UL36 genes of herpes simplex virus. In Current Research on Marek’s Disease, pp. 219-224. Edited by R. F. Silva, H. H. Cheng, P. M. Coussens, L. F. Lee & L. F. Velicer. Kennett Square, PA: American Association of Avian Pathologists.
  24. Wu, P., Lee, L. F. & Reed, W. M. ( 1997; ). Serological characteristics of a membrane glycoprotein gp82 of Marek’s disease virus. Avian Diseases 41, 824-831.[CrossRef]
    [Google Scholar]
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