1887

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Volume 80, Issue 8

Identification and functional analysis of a putative non- origin of DNA replication from the type B multinucleocapsid nucleopolyhedrovirus Free

Abstract

A putative non- origin of DNA replication was identified in the multinucleocapsid nucleopolyhedrovirus (SpliNPV) genome by transient replication assays. The putative SpliNPV was mapped to the I-J fragment between 75·1–77·9 map units in the SpliNPV genome. While the DNA sequence of the putative SpliNPV aligned with regions within the non-s of , and multinucleocapsid nucleopolyhedroviruses, it has limited DNA sequence identity with these elements. The sequence of the putative SpliNPV non- fragment contains a unique distribution of imperfect palindromes, multiple direct repeats and putative transcription factor-binding sites. Transient expression assays indicated that the putative SpliNPV fragment repressed SpliNPV promoter-mediated luciferase reporter gene expression. However, the putative SpliNPV fragment itself was capable of directing luciferase expression in the absence of a recognizable baculovirus promoter element in an orientation-independent fashion, suggesting that DNA sequence motifs within its sequence can activate transcription. Gel mobility shift analyses confirmed that proteins within nuclear extracts from both uninfected and virus-infected cells bound with specificity to the putative SpliNPV fragment.

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1999-08-01
2024-04-26
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References

  1. Ahrens C. H., Pearson M. N., Rohrmann G. F. 1995; Identification and characterization of a second putative origin of DNA replication in a baculovirus of Orgyia pseudotsugata. Virology 207:572–576
     [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. Journal of Molecular Biology 215:403–410
     [Google Scholar]
  3. Ayres M. D., Howard S. C., Kuzio J., Lopez-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605
     [Google Scholar]
  4. Blissard G. W., Rohrmann G. F. 1990; Baculovirus diversity and molecular biology. Annual Review of Entomology 35:127–155
     [Google Scholar]
  5. Boulikas T. 1996; Common structural features of replication origins in all life forms. Journal of Cellular Biochemistry 60:297–316
     [Google Scholar]
  6. Bradford M. M. 1976; Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72:248–254
     [Google Scholar]
  7. Broer R., Heldens J. G. M., van Strien E. A., Zuidema D., Vlak J. M. 1998; Specificity of multiple homologous genomic regions in Spodoptera exigua nucleopolyhedrovirus DNA replication. Journal of General Virology 79:1563–1572
     [Google Scholar]
  8. Carson D. D., Summers M. D., Guarino L. A. 1991; Molecular analysis of a baculovirus regulatory gene. Virology 182:279–286
     [Google Scholar]
  9. Cheng L., Kelly T. J. 1989; Transcriptional activator nuclear factor I stimulates the replication of SV40 minichromosomes in vivo and in vitro. Cell 59:541–551
     [Google Scholar]
  10. Croizier L., Croizier G. 1994; Nucleotide sequence of the polyhedrin gene of Spodoptera littoralis multiple nucleocapsid nuclear polyhedrosis virus. Biochimica et Biophysica Acta 1218:457–459
     [Google Scholar]
  11. Croizier G., Boukhoudmi-Amiri K., Croizier L. 1989; A physical map of Spodoptera littoralis B-type nuclear polyhedrosis virus genome. Archives of Virology 104:145–151
     [Google Scholar]
  12. DePamphilis M. L. 1988; Transcriptional elements as components of eukaryotic origin of DNA replication. Cell 52:635–638
     [Google Scholar]
  13. DePamphilis M. L. 1993; Eukaryotic DNA replication: anatomy of an origin. Annual Review of Biochemistry 62:29–63
     [Google Scholar]
  14. DePamphilis M. L. 1996; Eukaryotic replication origins. In DNA Replication in Eukaryotic Cells pp 45–86 Edited by DePamphilis M. L. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  15. Faktor O., Kamensky B. 1997; Genomic location and nucleotide sequence of a lef-8 gene of the Spodoptera littoralis nucleopolyhedrovirus. Virus Genes 15:9–15
     [Google Scholar]
  16. Faktor O., Toister-Achituv M., Kamensky B. 1995; Identification and nucleotide sequence of an ecdysteroid UDP-glucosyltransferase gene of Spodoptera littoralis multicapsid nuclear polyhedrosis virus. Virus Genes 11:47–52
     [Google Scholar]
  17. Faktor O., Toister-Achituv M., Nahum O. 1997a; Enhancer element, repetitive sequences, and gene organization in an 8-kbp region containing the polyhedrin gene of the Spodoptera littoralis nucleopolyhedrovirus. Archives of Virology 142:1–15
     [Google Scholar]
  18. Faktor O., Toister-Achituv M., Nahum O., Kamensky B. 1997b; The p10 gene of Spodoptera littoralis nucleopolyhedrovirus: nucleotide sequence, transcription analysis and unique gene organization in the p10 locus. Journal of General Virology 78:2119–2128
     [Google Scholar]
  19. Guarino L. A., Summers M. D. 1986; Interspersed homologous DNA of Autographa californica nuclear polyhedrosis virus enhances delayed-early gene expression. Journal of Virology 60:215–223
     [Google Scholar]
  20. Heldens J. G. M., Broer R., Zuidema D., Goldbach R. W., Vlak J. M. 1997; Identification and functional analysis of a non- hr origin of DNA replication in the genome of Spodoptera exigua multicapsid nucleopolyhedrovirus. Journal of General Virology 78:1497–1506
     [Google Scholar]
  21. Herendeen D. R., Kassavetis G. A., Barry J., Alberts B. M., Geiduschek E. P. 1989; Enhancement of bacteriophage T4 late transcription by components of the T4 DNA replication apparatus. Science 245:952–958
     [Google Scholar]
  22. Hu Z., Broer R., Westerlaken J., Martens J. W. M., Jin F., Wang L. M., Vlak J. M. 1997; Identification and nucleotide sequence of the ecdysteroid UDP-glycosyltransferase gene of the Buzura suppressaria nucleopolyhedrovirus. Virus Research 47:91–97
     [Google Scholar]
  23. Jones K. A., Kadonaga J. T., Rosenfeld P. J., Kelly T. J., Tjian R. 1987; A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication. Cell 73:1207–1221
     [Google Scholar]
  24. Kogan P. H., Blissard G. W. 1994; A baculovirus gp64 early promoter is activated by host transcription factor binding to CACGTG and GATA elements. Virology 119:219–222
     [Google Scholar]
  25. Kool M., Goldbach R. W., Vlak J. M. 1994; A putative non- hr origin of DNA replication in the Hin dIII-K fragment of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus. Journal of General Virology 75:3345–3352
     [Google Scholar]
  26. Kool M., Ahrens C. H., Vlak J. M., Rohrmann G. F. 1995; Replication of baculovirus DNA. Journal of General Virology 76:2103–2118
     [Google Scholar]
  27. Lee H. J., Krell P. J. 1994; Reiterated DNA fragments in defective genomes of Autographa californica nuclear polyhedrosis virus are competent for AcMNPV-dependent DNA replication. Virology 202:418–429
     [Google Scholar]
  28. Leisy D. J., Rohrmann G. F. 1993; Characterization of the replication of plasmids containing hr sequences in baculovirus-infected Spodoptera frugiperda cells. Virology 196:722–730
     [Google Scholar]
  29. Leisy D. J., Rasmussen C., Kim H. T., Rohrmann G. F. 1995; The Autographa californica nuclear polyhedrosis virus homologous region 1a: identical sequences are essential for DNA replication activity and transcriptional enhancer function. Virology 208:742–752
     [Google Scholar]
  30. Levin D. B., Laitinen A. M., Clarke T. C., Lucarotti C. J., Morin B., Otvos I. S. 1997; Characterization of nuclear polyhedrosis viruses from three subspecies of Lambdina fiscellaria. Journal of Invertebrate Pathology 69:125–134
     [Google Scholar]
  31. Li Y., Passarelli A. L., Miller L. K. 1993; Identification, sequence, and transcriptional mapping of lef-3 , a baculovirus gene involved in late and very late gene expression. Journal of Virology 67:5260–5268
     [Google Scholar]
  32. Lu A., Carstens E. B. 1993; Immediate-early baculovirus genes transactivate the p143 gene promoter of Autographa californica nuclear polyhedrosis virus. Virology 195:710–718
     [Google Scholar]
  33. Lu A., Krell P. J., Vlak J. M., Rohrmann G. F. 1997; Baculovirus DNA replication. In The Baculoviruses pp 171–191 Edited by Miller L. K. New York: Plenum;
     [Google Scholar]
  34. Majima K., Kobara R., Maeda S. 1993; Divergence and evolution of homologous regions of Bombyx mori nuclear polyhedrosis virus. Journal of Virology 67:7513–7521
     [Google Scholar]
  35. Mul Y. M., Verrijzer C. P., van der Vliet P. C. 1990; Transcription factors NFI and NFIII/OCT-1 function independently, employing different mechanisms to enhance adenovirus DNA replication. Journal of Virology 64:5510–5518
     [Google Scholar]
  36. Nguyen-Huynh A. T., Schaffer P. A. 1998; Cellular transcription factor enhance herpes simplex virus type 1 ori S-dependent DNA replication. Journal of Virology 72:3635–3645
     [Google Scholar]
  37. Nissen M. S., Friesen P. D. 1989; Molecular analysis of the transcriptional regulatory region of an early baculovirus gene. Journal of Virology 63:493–503
     [Google Scholar]
  38. O’Reilly D. R., Miller L. K., Luckow V. A. 1992 Baculovirus Expression Vectors. A Laboratory Manual New York: W. H. Freeman;
  39. Parker C. S., Topol J. 1984; A Drosophila RNA polymerase II transcription factor contains a promoter-region-specific DNA-binding activity. Cell 36:357–369
     [Google Scholar]
  40. Pearson M. N., Rohrmann G. F. 1995; Lymantria dispar nuclear polyhedrosis virus homologous regions: characterization of their ability to function as replication origins. Journal of Virology 69:213–221
     [Google Scholar]
  41. Pearson M. N., Bjornson R. M., Ahrens C., Rohrmann G. F. 1993; Identification and characterization of a putative origin of DNA replication in the genome of a baculovirus pathogenic for Orgyia pseudotsugata. Virology 197:715–725
     [Google Scholar]
  42. Pruijn G. J. M., van Miltenburg R. T., Claessens J. A. J., van der Vliet P. C. 1988; Interaction between the octamer-binding protein nuclear factor III and the adenovirus origin of DNA replication. Journal of Virology 62:3092–3102
     [Google Scholar]
  43. Quandt K., Frech K., Karas H., Wingender E., Werner T. 1995; MatInd and MatInspector – new fast and versatile tools for detection of consensus matches in nucleotide sequences. Nucleic Acids Research 23:4878–4884
     [Google Scholar]
  44. Rivier D. H., Rine J. 1992; An origin of DNA replication and a transcription silencer require a common element. Science 256:659–663
     [Google Scholar]
  45. Rodems S. M., Friesen P. D. 1993; The hr 5 transcriptional enhancer stimulates early expression from the Autographa californica nuclear polyhedrosis virus genome but is not required for virus replication. Journal of Virology 67:5776–5785
     [Google Scholar]
  46. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  47. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5468
     [Google Scholar]
  48. Sarisky R. T., Hayward G. S. 1996; Evidence that the UL84 gene product of human cytomegalovirus is essential for promoting ori Lyt-dependent DNA replication and formation of replication compartments in cotransfection assays. Journal of Virology 70:7398–7413
     [Google Scholar]
  49. Smith I., Goodale C. 1998; Sequence and in vivo transcription of Lacanobia oleracea granulovirus egt. Journal of General Virology 79:405–413
     [Google Scholar]
  50. Thompson J. D., Higgins D. G., Gibson T. J. 1994; Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
     [Google Scholar]
  51. van der Vliet P. C. 1996; Roles of transcription factors in DNA replication. In DNA Replication in Eukaryotic Cells pp. 87–118 Edited by DePamphilis M. L. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  52. Volkman L. E., Blissard G. W., Freisen P. D., Possee R. D., Theilmann D. A. 1995; The Baculoviridae. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp 104–113 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Vienna & New York: Springer-Verlag;
     [Google Scholar]
  53. Wolff J.-L. C., Herzog L. M., Sun L., Levin D. B. 1998; Identification and Characterization of the Spodoptera littoralis nucleopolyhedrovirus lef-3 gene. Archives of Virology 143:743–767
     [Google Scholar]
  54. Wu Y., Carstens E. B. 1996; Initiation of baculovirus DNA replication: early promoter regions can function as infection-dependent replicating sequences in a plasmid-based replication assay. Journal of Virology 70:6967–6972
     [Google Scholar]
  55. Xie W. D., Arif B., Dobos P., Krell P. J. 1995; Identification and analysis of a putative origin of DNA replication in the Choristoneura fumiferana multinucleocapsid nuclear polyhedrosis virus genome. Virology 209:409–419
     [Google Scholar]
  56. Zannotto P. M., Kessing B. D., Maruniak J. E. 1993; Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. Journal of Invertebrate Pathology 62:147–164
     [Google Scholar]
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Identification and functional analysis of a putative non-hr origin of DNA replication from the Spodoptera littoralis type B multinucleocapsid nucleopolyhedrovirus
J. Gen. Virol. 80, 2263 (1999); https://doi.org/10.1099/0022-1317-80-8-2263
/content/journal/jgv/10.1099/0022-1317-80-8-2263
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