1887

Abstract

A warm-water fish cell line developed from blotched snakehead caudal peduncle (BSN) was found to have persistent birnavirus infection. Purified virus particles were of icosahedral shape and had 57±1·6 nm diameter. The BSN virus was resistant to 5-iodo-2′-deoxyuridine and induced yellowish-green cytoplasmic inclusions when stained with acridine orange. The virus was resistant to chloroform, acid and alkaline pH and heat treatment at 56 °C for 2 h. Purified virions had a buoyant density of 1·33 g/ml in CsCl and contained two genomic segments with molecular masses of 2·56×10 and 2·00×10 Da and four structural polypeptides of 112 (polyprotein, PP), 91 (VP1), 44 (VP2) and 37 (VP3) kDa. Reciprocal β cross-neutralization tests incorporating four classical strains of infectious pancreatic necrosis virus (IPNV) (WB, Sp, Ab and TV-1) and the BSN virus established the complete serological distinctness of the virus from IPNV. Considering the uniqueness of the virus, the name blotched snakehead virus is proposed for this agent.

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1999-08-01
2024-05-06
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References

  1. Amend D. F., McDowell T., Hedrick R. P. 1984; Characteristics of a previously unidentified virus from channel catfish ( Ictalurus punctatus. Canadian Journal of Fisheries and Aquatic Sciences 41:807–811
    [Google Scholar]
  2. Archetti I., Horsfall F. L. Jr 1950; Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum. Journal of Experimental Medicine 92:441–462
    [Google Scholar]
  3. Burleson F. G., Chambers T. M., Wiedbrauk D. L. 1992 Virology: A Laboratory Manual pp 186–246 London: Academic Press;
  4. Chen M. M., Kou G. H., Chen S. N. 1993; Establishment and characterization of a cell line persistently infected with infectious pancreatic necrosis virus (IPNV. Bulletin of the Institute of Zoology, Academia Sinica 32:265–272
    [Google Scholar]
  5. Dobos P. 1996; The molecular biology of infectious pancreatic necrosis virus (IPNV. Annual Review of Fish Diseases 5:25–54
    [Google Scholar]
  6. Dobos P., Hallett R., Kells D. T. C., Sorensen O., Rowe D. 1977; Biophysical studies of infectious pancreatic necrosis virus. Journal of Virology 22:150–159
    [Google Scholar]
  7. Dobos P., Hill B. J., Hallett R., Kells D. T. C., Becht H., Teninges D. 1979; Biophysical and biochemical characterization of five animal viruses with bisegmented double-stranded RNA genomes. Journal of Virology 32:593–605
    [Google Scholar]
  8. Dobos P., Nagy E., Duncan R. 1991; Birnaviridae. In Viruses of Invertebrates pp 301–314 Edited by Kurstak E. New York: Marcel Dekker;
    [Google Scholar]
  9. Dobos P., Berthiaume L., Leong J. A., Kibenge F. S. B., Muller H., Nicholson B. L. 1995; Family Birnaviridae. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp 240–244 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Vienna & New York: Springer-Verlag;
    [Google Scholar]
  10. Duncan R., Dobos P. 1986; The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large ORF encoding a precursor polyprotein. Nucleic Acids Research 14:5934
    [Google Scholar]
  11. Feldman H., Wang S. 1961; Sensitivity of various viruses to chloroform. Proceedings of the Society for Experimental Biology and Medicine 106:736–738
    [Google Scholar]
  12. Frerichs G. N., Morgan D., Hart D., Skerrow C., Roberts R. J., Onions D. E. 1991; Spontaneously productive C-type retrovirus infection of fish cell lines. Journal of General Virology 72:2537–2539
    [Google Scholar]
  13. Hedrick R. P., Eaton W. D., Fryer J. L., Groberg W. G. Jr, Boonyaratpalin S. 1986; Characteristics of a birnavirus isolated from cultured sandgoby, Oxyeleotris marmoratus. Diseases of Aquatic Organisms 1:219–225
    [Google Scholar]
  14. Hill B. J., Way K. 1996; Serological classification of infectious pancreatic necrosis (IPN) virus and other aquatic birnaviruses. Annual Review of Fish Diseases 5:55–77
    [Google Scholar]
  15. Kärber G. 1931; Beitrag zur kollektiven behandlung pharmakologischer Reihenversuche. Archives of Experimental Pathology and Pharmacology 162:480–483
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  17. Macdonald R. D., Gower D. A. 1981; Genomic and phenotypic divergence among three serotypes of aquatic birnaviruses (infectious pancreatic necrosis virus. Virology 114:187–195
    [Google Scholar]
  18. Magyar G., Dobos P. 1994; Expression of infectious pancreatic necrosis virus polyprotein and VP1 in insect cells and the detection of the polyprotein in purified virus. Virology 198:437–445
    [Google Scholar]
  19. Mahy B. W. J., Kangro H. O. 1996 Virology Methods Manual London: Academic Press;
  20. Okamoto N., Sano T., Hedrick R. P., Fryer J. L. 1983; Antigenic relationships of selected strains of infectious pancreatic necrosis virus and European eel virus. Journal of Fish Diseases 6:19–25
    [Google Scholar]
  21. Roberts R. J., Frerichs G. N., Tonguthai K., Chinabut S. 1994; Epizootic ulcerative syndrome of farmed and wild fishes. In Recent Advances in Aquaculture pp 207–239 Edited by Muir J. F., Roberts R. J. Oxford: Blackwell Science;
    [Google Scholar]
  22. Rovozzo G. C., Burke C. N. 1973 A Manual of Basic Virological Techniques pp 126–163 Englewood Cliffs, NJ: Prentice Hall;
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Wolf K., Quimby M. C. 1969; Fish cell line and tissue culture. In Fish Physiology pp 253–305 Edited by Hoar W. S., Randall D. J. New York: Academic Press;
    [Google Scholar]
  25. Wolf K., Gravell M., Malsberger R. G. 1966; Lymphocystis virus: isolation and propagation in centrarchid fish cell lines. Science 151:1004–1005
    [Google Scholar]
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