1887

Abstract

The genes of the and multicapsid nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and characterized. Both genes were similar to other baculovirus genes and to entomopoxvirus fusolin genes. Phylogenetic analysis showed that baculovirus genes and entomopoxvirus fusolin genes form two distinct and well-separated clades. There was no evidence of recent gene transfer between the two groups. The genes also showed a distant similarity to bacterial cellulose- and chitin-binding protein genes, but the significance of this is unclear. MbMNPV and LdMNPV were both transcribed from consensus baculovirus late transcription start sites. MbMNPV was additionally transcribed from a putative early transcription start site. Tunicamycin treatment of MbMNPV-infected cells confirmed that MbMNPV GP37 is -glycosylated. Confocal immunofluorescence microscopy revealed that the protein is located exclusively in the cytoplasm, probably in the endoplasmic reticulum.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-7-1823
1999-07-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/7/0801823a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-7-1823&mimeType=html&fmt=ahah

References

  1. Afonso C. L., Tulman E. R., Lu Z., Oma E., Kutish G. F., Rock D. L. 1999; The genome of Melanoplus sanguinipes entomopoxvirus. Journal of Virology 73:533–552
    [Google Scholar]
  2. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  3. Clarke E. E., Tristem M., Cory J. S., O’Reilly D. R. 1996; Characterization of the ecdysteroid UDP-glucosyltransferase gene from Mamestra brassicae nucleopolyhedrovirus. Journal of General Virology 77:2865–2871
    [Google Scholar]
  4. Dall D., Sriskantha A., Vera A., Lai-Fook J., Symonds T. 1993; A gene encoding a highly expressed spindle body protein of Heliothis armigera entomopoxvirus. Journal of General Virology 74:1811–1818
    [Google Scholar]
  5. Derksen A. C. G., Granados R. R. 1988; Alteration of a lepidopteran peritrophic membrane by baculoviruses and enhancement of viral infectivity. Virology 167:242–250
    [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  7. Gallo L. G., Corsaro B. G., Hughes P. R., Granados R. R. 1991; In vivo enhancement of baculovirus infection by the viral enhancing factor of a granulosis virus of the cabbage looper, Trichoplusia ni (Lepidoptera, Noctuidae). Journal of Invertebrate Pathology 58:203–210
    [Google Scholar]
  8. Garda A. L., Fernández-Abalos J. M., Sánchez P., Ruiz-Arribas A., Santimaría R. I. 1997; Two genes encoding an endoglucanase and a cellulose-binding protein are clustered and co-regulated by a TTA codon in Streptomyces halstedii JM8. Biochemical Journal 324:403–411
    [Google Scholar]
  9. Gauthier L., Cousserans F., Veyrunes J. C., Bergoin M. 1995; The Melolontha melolontha entomopoxvirus (MmEPV) fusolin is related to the fusolins of lepidopteran EPVs and to the 37K baculovirus glycoprotein. Virology 208:427–436
    [Google Scholar]
  10. Goodwin R. H., Tompkins G. J., McCawley P. 1978; Gypsy moth cell lines divergent in viral susceptibility. I. Culture and identification. In Vitro 14:485–494
    [Google Scholar]
  11. Goodwin R. H., Milner R. J., Beaton C. D. 1991; Entomopox-virinae. In Atlas of Invertebrate Viruses pp 393–434 Edited by Adams J. R., Bonami J. R. Boca Raton, FL: CRC Press;
    [Google Scholar]
  12. Gross C. H., Wolgamot G. M., Russell R. L. Q., Pearson M. N., Rohrmann G. F. 1993; A 37-kilodalton glycoprotein from a baculovirus of Orgyia pseudotsugata is localized to cytoplasmic inclusion bodies. Journal of Virology 67:469–475
    [Google Scholar]
  13. Harlow E., Lane D. 1988 Antibodies: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Hayakawa T., Xu J., Hukuhara T. 1996; Cloning and sequencing of the gene for an enhancing factor from Pseudaletia separata entomopox-virus. Gene 177:269–270
    [Google Scholar]
  15. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  16. Hink W. F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni . Nature 226:466–467
    [Google Scholar]
  17. Hunter F. R., Crook N. E., Entwistle P. F. 1984; Viruses as pathogens for the control of insects. In Microbiological Methods for Environmental Biotechnology pp 323–347 Edited by Grainger J. M., Lynch J. M. London: Academic Press;
    [Google Scholar]
  18. Kang W., Tristem M., Maeda S., Crook N. E., O’Reilly D. R. 1998; Identification and characterization of the Cydia pomonella granulovirus cathepsin and chitinase genes. Journal of General Virology 79:2283–2292
    [Google Scholar]
  19. Kishino H., Hasegawa M. 1989; Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea. Journal of Molecular Evolution 29:170–179
    [Google Scholar]
  20. Kuzio J., Pearson M. N., Harwood S. H., Funk C. J., Evans J. T., Slavicek J. M., Rohrmann G. F. 1999; Sequence and analysis of the genome of a baculovirus pathogenic for Lymantria dispar . Virology 253:17–34
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  22. Liu J. J., Carstens E. B. 1996; Identification, molecular cloning, and transcription analysis of the Choristoneura fumiferana nuclear polyhedrosis virus spindle-like protein gene. Virology 223:396–400
    [Google Scholar]
  23. Miller D. P., Dall D. J., O’Reilly D. R. 1999; Insect pest control by viruses. In Encyclopedia of Virology 2nd edn Edited by Webster R., Granoff A. San Diego: Academic Press; (in press)
    [Google Scholar]
  24. Mitsuhashi W., Saito H., Sato M. 1997; Complete nucleotide sequence of the fusolin gene of an entomopoxvirus in the cupreous chafer, Anomala cuprea Hope (Coleoptera: Scarabaeidae). Insect Biochemistry and Molecular Biology 27:869–876
    [Google Scholar]
  25. Mitsuhashi W., Furuta Y., Sato M. 1998; The spindles of an entomopoxvirus of coleoptera (Anomala cuprea) strongly enhance the infectivity of a nucleopolyhedrovirus in lepidoptera. Journal oflnvertebrate Pathology 71:186–188
    [Google Scholar]
  26. O’Reilly D. R., Miller L. K., Luckow V. A. 1992 Baculovirus Expression Vectors – A Laboratory Manual New York: Oxford University Press;
    [Google Scholar]
  27. Possee R. D., Kelly D. C. 1988; Physical maps and comparative DNA hybridization of Mamestra brassicae and Panolis flammea nuclear polyhedrosis virus genomes. Journal of General Virology 69:1285–1298
    [Google Scholar]
  28. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  30. Schnellmann J., Zeltins A., Blaak H., Schrempf H. 1994; The novel lectin-like protein CHB1 is encoded by a chitin-inducible Streptomyces olivaceoviridis gene and binds specifically to crystalline alpha-chitin of fungi and other organisms. Molecular Microbiology 13:807–819
    [Google Scholar]
  31. Slavicek J. M., Mercer M. J., Kelly M. E., Hayes-Plazolles N. 1996; Isolation of a baculovirus variant that exhibits enhanced polyhedra production during serial passage in cell culture. Journal of Invertebrate Pathology 67:153–160
    [Google Scholar]
  32. Sriskantha A., Osborne R. J., Dall D. J. 1997; Mapping of the Heliothis armigera entomopoxvirus (HaEPV) genome, and analysis of genes encoding the HaEPV spheroidin and nucleoside triphosphate phosphohydrolase I proteins. Journal of General Virology 78:3115–3123
    [Google Scholar]
  33. Templeton A. R. 1983; Phylogenetic inference from restriction endonuclease cleavage site maps with particular reference to the evolution of humans and the apes. Evolution 37:221–224
    [Google Scholar]
  34. Thiem S. M., Du X., Quentin M. E., Berner M. M. 1996; Identification of a baculovirus gene that promotes Autographa californica nuclear polyhedrosis virus replication in a nonpermissive insect cell line. Journal of Virology 70:2221–2229
    [Google Scholar]
  35. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
    [Google Scholar]
  36. Vialard J. E., Yuen L., Richardson C. D. 1990; Identification and characterization of a baculovirus occlusion body glycoprotein which resembles spheroidin, an entomopoxvirus protein. Journal of Virology 64:5804–5811
    [Google Scholar]
  37. Wang P., Granados R. R. 1997; An intestinal mucin is the target substrate for a baculovirus enhancin. Proceedings of the National Academy of Sciences, USA 94:6977–6982
    [Google Scholar]
  38. Wu J., Miller L. K. 1989; Sequence, transcription and translation of a late gene of the Autographa californica nuclear polyhedrosis virus encoding a 34.8K polypeptide. Journal of General Virology 70:2449–2459
    [Google Scholar]
  39. Yuen L., Dionne J., Arif B., Richardson C. 1990; Identification and sequencing of the spheroidin gene of Choristoneura biennis entomopox-virus. Virology 175:427–433
    [Google Scholar]
  40. Zanotto P. M. D., Kessing B. D., Maruniak J. E. 1992; Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. Journal of lnvertebrate Pathology 62:147–164
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-7-1823
Loading
/content/journal/jgv/10.1099/0022-1317-80-7-1823
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error