1887

Abstract

The complete nucleotide sequences of the human papillomavirus type 16 (HPV-16) variants present in the CaSki and SiHa cervical carcinoma cell lines and the primary subgenomic HPV-18 variant present in the HeLa cervical carcinoma cell line were determined using overlapping bulk PCR products as templates. PCR-based methods were also used to characterize five previously unreported CaSki HPV-16 genomic disruptions and the 5′ cellular-viral junction common to all HeLa HPV-18 subgenomic structures.

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1999-07-01
2021-11-28
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References

  1. Adler K., Erickson T., Bobrow M. 1997; High sensitivity detection of HPV-16 in SiHa and CaSki cells utilizing FISH enhanced by TSA. Histochemistry and Cell Biology 108:321–324
    [Google Scholar]
  2. Baker C. C. 1993; The genomes of the papillomaviruses. In Genetic Maps: Locus Maps of Complex Genomes 6th edn, pp 1.134–1.146 Edited by O’Brien S. J. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  3. Baker C. C., Phelps W. C., Lindgren V., Braun M. J., Gonda M. A., Howley P. M. 1987; Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines. Journal of Virology 61:962–971
    [Google Scholar]
  4. Bauer-Hoffman R., Borghouts C., Auvinen E., Bourda E., Rosl F., Alonso A. 1996; Genomic cloning and characterization of the nonoccupied allele corresponding to the integration site of human papillomavirus type 16 DNA in the cervical cancer cell line SiHa. Virology 217:33–41
    [Google Scholar]
  5. Bosch F. X., Manos M. M., Munoz N., Sherman M., Jansen A. M., Peto J., Schiffman M. H., Moreno V., Kurman R., Shah K. V., C I. B. S. C. Study Group 1995; Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. Journal of the National Cancer Institute 87:796–802
    [Google Scholar]
  6. Boshart M., Gissmann L., Ikenberg H., Kleinheinz A., Scheurlen W., zur Hausen H. 1984; A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO Journal 3:1151–1157
    [Google Scholar]
  7. Callahan D. E., Karim A., Zheng G., Ts’o P. O., Lesko S. A. 1992; Quantitation and mapping of integrated human papillomavirus on human metaphase chromosomes using a fluorescence microscope imaging system. Cytometry 13:453–461
    [Google Scholar]
  8. Carmody M. W., Jones M., Tarraza H., Vary C. P. H. 1996; Use ofthe polymerase chain reaction to specifically amplify integrated HPV-16 DNA by virtue of its linkage to interspersed repetitive DNA. Molecular and Cellular Probes 10:107–116
    [Google Scholar]
  9. Chan S.-Y., Ho L., Ong C.-K., Chow V., Drescher B., Durst M., ter Meulen J., Villa L., Luande J., Mgaya H. N., Bernard H.-U. 1992; Molecular variants of human papillomavirus type 16 from four continents suggest ancient pandemic spread of the virus and its coevolution with humankind. Journal of Virology 66:2057–2066
    [Google Scholar]
  10. Choo K.-B., Chen C.-M., Han C.-P., Cheng W. T. K., Au L.-C. 1996; Molecular analysis of cellular loci disrupted by papillomavirus 16 integration in cervical cancer: frequent viral integration in topologically destabilized and transcriptionally active chromosomal regions. Journal of Medical Virology 49:15–22
    [Google Scholar]
  11. Cole S. T., Danos O. 1987; Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Journal of Molecular Biology 193:599–608
    [Google Scholar]
  12. El Awady M. K., Kaplan J. B., O’Brien S. J., Burk R. D. 1987; Molecular analysis of integrated human papillomavirus 16 sequences in the cervical cancer cell line SiHa. Virology 159:389–398
    [Google Scholar]
  13. Forslund O., Hansson B. G. 1996; Human papillomavirus type 70 genome cloned from overlapping PCR products: complete nucleotide sequence and genomic organization. Journal of Clinical Microbiology 34:802–809
    [Google Scholar]
  14. Friedl F., Kimura I., Osato T., Ito Y. 1970; Studies on a new human cell line (SiHa) derived from carcinoma of uterus. I. Its establishment and morphology. Proceedings ofthe Societyfor Experimental Biology and Medicine 135:543–545
    [Google Scholar]
  15. Geisen C., Kahn T. 1996; Promoter activity of sequences located upstream of the human papillomavirus types 16 and 18 late regions. Journal of General Virology 77:2193–2200
    [Google Scholar]
  16. Gey G. O., Coffman W. D., Kubicek M. T. 1952; Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Research 12:264–265
    [Google Scholar]
  17. Hu G., Liu W., Mendelsohn J., Ellis L. M., Radinsky R., Andreeff M., Deisseroth A. B. 1997; Expression of epidermal growth factor receptor and human papillomavirus E6/E7 proteins in cervical carcinoma cells. Journal of the National Cancer Institute 89:1271–1276
    [Google Scholar]
  18. Human Papillomaviruses 1995 A Compilation and Analysis of Nucleic Acid and Amino Acid Sequences Edited by Myers G., Delius H., Icenogle J., Bernard H.-U., Baker C., Halpern A., Wheeler C. Los Alamos, NM: Theoretical Biology and Biophysics, Los Alamos National Laboratory;
    [Google Scholar]
  19. Human Papillomaviruses 1996 A Compilation and Analysis of Nucleic Acid and Amino Acid Sequences Edited by Myers G., Baker C., Wheeler C., Halpern A., McBride A., Doorbar J. Los Alamos, NM: Theoretical Biology and Biophysics, Los Alamos National Laboratory;
    [Google Scholar]
  20. Icenogle J. P., Sathya P., Miller D. L., Tucker R. A., Rawls W. E. 1991; Nucleotide and amino acid sequence variation in the L1 and E7 open reading frames of human papillomavirus type 6 and type 16. Virology 184:101–107
    [Google Scholar]
  21. Inagaki Y., Tsunokawa Y., Takebe N., Nawa H., Nakanishi S., Terada M., Sugimura T. 1988; Nucleotide sequences of cDNAs for human papillomavirus type 18 transcripts in HeLa cells. Journal of Virology 62:1640–1646
    [Google Scholar]
  22. Jeon S., Lambert P. R. 1995; Integration of human papillomavirus type 16 DNA into the human genome leads in increased stability of E6 and E7 mRNAs: implications for cervical carcinogenesis. Proceedings of the National Academy of Sciences, USA 92:1654–1658
    [Google Scholar]
  23. Jeon S., Allen-Hoffman B. L., Lambert P. F. 1995; Integration of human papillomavirus type 16 into the human genome correlates with a selective growth advantage of cells. Journal of Virology 69:2989–2997
    [Google Scholar]
  24. Jones H. W. Jr, McKusick V. A., Harper P. S., Wuu K.-D. 1971; The HeLa cell and a reappraisal of its origin. Obstetrics and Gynecology 38:945–959
    [Google Scholar]
  25. Lappalainen K., Pirila L., Jaaskelainen I., Syrjanen K., Syrjanen S. 1996; Effects of liposomal antisense oligonucleotides on mRNA and protein levels of the HPV 16 E7 oncogene. Anticancer Research 16:2485–2492
    [Google Scholar]
  26. Lazo P. A. 1987; Structure, DNaseI hypersensitivity and expression of integrated papilloma virus in the genome of HeLa cells. European Journal of Biochemistry 165:393–401
    [Google Scholar]
  27. Lazo P. A. 1988; Rearrangement ofboth alleles of human chromosome 8 in HeLa cells, one of them as a result of papillomavirus DNA integration. Journal of Biological Chemistry 263:360–367
    [Google Scholar]
  28. List H. J., Patzel V., Zeidler U., Schopen A., Ruhl G., Stollwerk J., Klock G. 1994; Methylation sensitivity of the enhancer from the human papillomavirus type 16. Journal of Biological Chemistry 269:11902–11911
    [Google Scholar]
  29. Lorincz A. T., Reid R., Jenson A. B., Greenberg M. D., Lancaster W., Kurman R. J. 1992; Human papillomavirus infection of the cervix: relative risk associations of 15 common anogenital types. Obstetrics and Gynecology 79:328–337
    [Google Scholar]
  30. Maki H., Fujikawa-Adache I., Yoshie O. 1996; Evidence for a promoter-like activity in the short non-coding region of human papillomaviruses. Journal of General Virology 77:453–458
    [Google Scholar]
  31. Meissner J. 1993 Taql is a single-cut enzyme for HPV-18. Nucleic Acids Research 21:1041
    [Google Scholar]
  32. Meissner J. 1997; Sequencing errors in reference HPV clones. In Human Papillomaviruses 1997: A Compilation and Analysis of Nucleic Acid and Amino Acid Sequences pp III-110–III-123 Edited by Myers G., Baker C., Munger K., Sverdrup F., McBride A. Bernard H.-U. Meissner J. Los Alamos, NM: Theoretical Biology and Biophysics, Los Alamos National Laboratory;
    [Google Scholar]
  33. Mincheva A., Gissmann L., zur Hausen H. 1987; Chromosomal integration sites of human papillomavirus DNA in three cervical cancer cell lines mapped by in situ hybridization. Medical Microbiology and Immunology 176:245–256
    [Google Scholar]
  34. Ong C.-K., Chan S.-Y., Campo M. S., Fujinaga K., Mavromara-Nazos P., Labropoulou V., Pfister H., Tay S.-K., ter Meulen J., Villa L. L., Bernard H.-U. 1993; Evolution of human papillomavirus type 18: an ancient phylogenetic root in Africa and intratype diversity reflect coevolution with human ethnic groups. Journal of Virology 67:6424–6431
    [Google Scholar]
  35. Pack R. A., Tsurimoto T. 1994; Isolation of human DNA-unwinding elements as sites of DNA polymerase a/primase entry. Gene 148:277–284
    [Google Scholar]
  36. Parker L. T., Zakeri H., Deng Q., Spurgeon S., Kwok P.-Y., Nickerson D. A. 1996; AmpliTaq DNA polymerase, FS dye-terminator sequencing: analysis of peak height patterns. BioTechniques 21:694–699
    [Google Scholar]
  37. Pater M. M., Pater A. 1985; Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145:313–318
    [Google Scholar]
  38. Pattillo R. A., Hussa R. O., Story M. T., Ruckert A. C. F., Shalaby M. R., Mattingly R. F. 1977; Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Science 196:1456–1458
    [Google Scholar]
  39. Picken R. N., Yang H.-L. 1987; The integration of HPV-18 into HeLa cells has involved duplication of part of the viral genome as well as human DNA flanking sequences. Nucleic Acids Research 15:10068
    [Google Scholar]
  40. Popescu N. C., DiPaolo J. A. 1989; Preferential sites for vial integration on mammalian genome. Cancer Genetics and Cytogenetics 42:157–171
    [Google Scholar]
  41. Rosl F., Westphal E.-M., zur Hausen H. 1989; Chromatin structure and transcriptional regulation of human papillomavirus type 18 DNA in HeLa cells. Molecular Carcinogenesis 2:72–80
    [Google Scholar]
  42. Rosl F., Arab A., Klevenz B., zur Hausen H. 1993; The effect of DNA methylation on gene regulation of human papillomaviruses. Journal of General Virology 74:791–801
    [Google Scholar]
  43. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  44. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  45. Schneider-Gadicke A., Schwarz E. 1986; Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO Journal 5:2285–2292
    [Google Scholar]
  46. Schneider-Maunoury S., Croissant O., Orth G. 1987; Integration of human papillomavirus type 16 DNA sequences: a possible early event in the progression of genital tumors. Journal of Virology 61:3295–3298
    [Google Scholar]
  47. Schwarz E., Freese U. K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., zur Hausen H. 1985; Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature 314:111–114
    [Google Scholar]
  48. Smits H. L., Cornelissen M. T., Jebbink M. F., van den Tweel J. G., Struyk A. P., Briet M., ter Schegget J. 1991; Human papillomavirus type 16 transcripts expressed from viral-cellular junctions and full-length viral copies in CaSki cells and in a cervical carcinoma. Virology 182:870–873
    [Google Scholar]
  49. Smotkin D., Wettstein F. O. 1986; Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein. Proceedings of the National Academy of Sciences, USA 83:4680–4684
    [Google Scholar]
  50. Stewart A.-C. M., Gravitt P. E., Cheng S., Wheeler C. M. 1995; Generation of entire human papillomavirus genomes by long PCR: frequency of errors produced during amplification. Genome Research 5:79–88
    [Google Scholar]
  51. Swift F. V., Bhat K., Younghusband H. B., Hamada H. 1987; Characterization of a cell type-specific enhancer found in the human papilloma virus type 18 genome. EMBO Journal 6:1339–1344
    [Google Scholar]
  52. Tan S. H., Bartsch D., Schwarz E., Bernard H.-U. 1998; Nuclear matrix attachment regions of human papillomavirus type 16 point toward conservation of these genomic elements in all genital papillomaviruses. Journal of Virology 72:3610–3622
    [Google Scholar]
  53. ter Meulen J., Schweigler A. C., Eberhardt H. C., Luande J., Mgaya H. N., Muller M., Bleul C., Ulken V., Ikenberg H., Pawlita M., Gissmann L. 1993; Sequence variation in the E7 gene of human papillomavirus type 18 in tumor and non-tumor patients and antibody response to a conserved seroreactive epitope. International Journal of Cancer 53:257–259
    [Google Scholar]
  54. Tornesello M. L., Buonaguro F. M., Meglio A., Buonaguro L., Beth-Giraldo E., Giraldo G. 1997; Sequence variations and viral genomic state of human papillomavirus type 16 in penile carcinomas from Ugandan patients. Journal of General Virology 78:2199–2208
    [Google Scholar]
  55. Xi L. F., Koutsky L. A., Galloway D. A., Kuypers J., Hughes J. P., Wheeler C. M., Holmes K. K., Kiviat N. B. 1997; Genomic variation of human papillomavirus type 16 and risk for high grade cervical intraepithelial neoplasia. Journal of the National Cancer Institute 89:796–802
    [Google Scholar]
  56. Yamada T., Wheeler C. M., Halpern A. L., Stewart A.-C. M., Hilde-sheim A., Jenison S. A. 1995; Human papillomavirus type 16 variant lineages in United States populations characterized by nucleotide sequence analysis of the E6, L2, and L1 coding segments. Journal of Virology 69:7743–7753
    [Google Scholar]
  57. Yamada T., Manos M. M., Peto J., Greer C. E., Munoz N., Bosch F. X., Wheeler C. M. 1997; Human papillomavirus type 16 sequence variation in cervical cancers: a worldwide perspective. Journal of Virology 61:2463–2472
    [Google Scholar]
  58. Yee C., Krishnan-Hewlett I., Baker C. C., Schlegel R., Howley P. M. 1985; Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. American Journal of Pathology 119:361–366
    [Google Scholar]
  59. Yuan J., Yeasky T. M., Rhee M. C., Glazer P. M. 1995; Frequent T: A → G: C transversions in X-irradiated mouse cells. Carcinogenesis 16:83–88
    [Google Scholar]
  60. zur Hausen H. 1994; Molecular pathogenesis of cancer of the cervix and its causation by specific human papillomavirus types. Current Topics in Microbiology and Immunology 186:131–156
    [Google Scholar]
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