1887

Abstract

We have started to characterize the capsid components of European Chlorella virus isolate CVG-1, a member of the Pbi subgroup of the . The major coat protein, Vp49, was biochemically characterized and the amino acid sequence of the N terminus was determined. Subsequently, the corresponding gene was isolated from CVG-1 genomic DNA. Sequence data were compared to those available from PBCV-1 and other Chlorella virus isolates representing the NC64A subgroup of the . The major coat proteins of all strains are homologous and similar in size, but apparently differ in their degree of glycosylation. Like PBCV-1, the major coat protein of CVG-1 is part of a gene family, as two open reading frames with high similarity to Vp49 were also isolated and characterized in this study. The predicted amino acid sequences of the CVG-1 and PBCV-1 virus genes examined show, with one exception, a divergence of about 25%. Taking into account that corresponding genes of NC64A viruses are almost identical, this divergence supports the original placement of the NC64A and Pbi viruses into separate subgroups of the .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-4-1067
1999-04-01
2024-12-09
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/4/0801067a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-4-1067&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment tool. Journal of Molecular Biology 215:403–410
    [Google Scholar]
  2. Bairoch A., Bucher P., Hofmann K. 1997; The PROSITE database and its status in 1997. Nucleic Acids Research 25:217–221
    [Google Scholar]
  3. Chen F., Suttle C. A. 1996; Evolutionary relationships among large double-stranded DNA viruses that infect microalgae and other organisms as inferred from DNA polymerase genes. Virology 219:170–178
    [Google Scholar]
  4. Church G. M., Gilbert W. 1984; Genomic sequencing. Proceedings of the National Academy of Sciences, USA 81:1991–1995
    [Google Scholar]
  5. Graves M. V., Meints R. H. 1992; Characterization of the major capsid protein and cloning of its gene from algal virus PBCV-1. Virology 188:198–207
    [Google Scholar]
  6. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple alignments on a microcomputer. Gene 73:237–244
    [Google Scholar]
  7. Kapitany R. A., Zebrowski E. J. 1973; A high resolution PAS stain for polyacrylamide gel electrophoresis. Analytical Biochemistry 56:361–369
    [Google Scholar]
  8. Lu Z. Q., Li Y., Zhang Y. P., Kutish G. F., Rock D. L., Van Etten J. L. 1995; Analysis of 45 kb of DNA located at the left end of the chlorella virus PBCV-1 genome. Virology 206:339–352
    [Google Scholar]
  9. Li Y., Lu Z. Q., Sun L., Ropp S., Kutish G., Rock D. L., Van Etten J. L. 1997; Analysis of 76 kb of DNA located at the right end of the 330-kb chlorella virus PBCV-1 genome. Virology 237:360–377
    [Google Scholar]
  10. Macaulay C., McFadden G. 1989; Tumorigenic poxviruses: characterization of an early promotor from Shope fibroma virus. Virology 172:237–246
    [Google Scholar]
  11. Nishida K., Suzuki S., Kimura Y., Nomura N., Fujie M., Yamada T. 1998; Group I introns found in chlorella viruses: biological implications. Virology 242:319–326
    [Google Scholar]
  12. Reisser W., Widowski M. 1992; Taxonomy of eukaryotic algae endosymbiotic in freshwater associations. In Algae and Symbioses pp 21–40 Edited by Reisser W. Bristol: Biopress;
    [Google Scholar]
  13. Reisser W., Becker B., Klein T. 1986; Studies on ultrastructure and host range of a Chlorella attacking virus. Protoplasma 135:162–165
    [Google Scholar]
  14. Reisser W., Burbank D. E., Meints S. M., Meints R. H., Becker B., Van Etten J. L. 1988; A comparison of viruses infecting two different Chlorella-like green algae. Virology 167:143–149
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Schuster A. M., Graves M., Korth K., Ziegelbein M., Brumbaugh J., Grone D., Meints R. H. 1990; Transcription and sequence studies of a 4′3-kbp fragment from a ds-DNA eukaryotic algal virus. Virology 176:515–523
    [Google Scholar]
  17. Skrdla M. P., Burbank D. E., Xia Y., Meints R. H., Van Etten J. L. 1984; Structural proteins and lipids in a virus, PBCV-1, which replicates in a Chlorella-like alga. Virology 135:308–315
    [Google Scholar]
  18. Songsri P., Hiramatsu S., Fujie M., Yamada T. 1997; Proteolytic processing of chlorella virus CVK2 capsid proteins. Virology 227:252–254
    [Google Scholar]
  19. Van Etten J. L., Burbank D. E., Xia Y., Meints R. H. 1983; Growth cycle of a virus, PBCV-1, that infects a Chlorella-like algae. Virology 126:117–125
    [Google Scholar]
  20. Van Etten J. L., Lane L. C., Meints R. H. 1991; Viruses and viruslike particles of eukaryotic algae. Microbiological Reviews 55:586–620
    [Google Scholar]
  21. Wang I. N., Li Y., Que Q., Bhattacharya M., Lane L. C., Chaney W. G., Van Etten J. L. 1993; Evidence for virus-encoded glycosylation specificity. Proceedings of the National Academy of Sciences, USA 90:3840–3844
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-80-4-1067
Loading
/content/journal/jgv/10.1099/0022-1317-80-4-1067
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error