1887

Abstract

The aim of this study was to investigate the importance of cellular immunity in foot-and-mouth disease in cattle, in particular to determine whether a CD8+ T-cell response could be detected, as these cells may play a role in both immunity and virus persistence. As attempts to characterize classical cytotoxic T cells had yielded non-reproducible results, largely due to high backgrounds in control cultures, a proliferation assay was developed that was demonstrated to detect antigen-specific, MHC class I-restricted bovine CD8+ cells responding to foot-and-mouth disease virus (FMDV). Proliferative CD8+ T-cell responses were detected consistently from 10 to 14 days following infection with FMDV and typically lasted 3-4 weeks. The role of CD8+ T cells in control of the disease, in particular their relevance for the establishment of persistence, may now be investigated.

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1999-03-01
2021-10-26
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References

  1. Amadori M., Archetti I. L., Verardi R., Berneri C. 1992a; Target recognition by bovine mononuclear, MHC-unrestricted cytotoxic cells. Veterinary Microbiology 33:383–392
    [Google Scholar]
  2. Amadori M., Archetti I. L., Verardi R., Berneri C. 1992b; Isolation of mononuclear cytotoxic cells from cattle vaccinated against foot-and-mouth disease. Archives of Virology 122:293–306
    [Google Scholar]
  3. Amadori M., Archetti I. L., Verardi R., Berneri C. 1995; Role of a distinct population of bovine γδ T cells in the immune response to viral agents. Viral Immunology 8:81–91
    [Google Scholar]
  4. Becker Y. 1994; Need for cellular and humoral immune responses in bovines to ensure protection from foot-and-mouth disease virus (FMDV)–a point of view. Virus Genes 8:199–214
    [Google Scholar]
  5. Borrow P., Tonks P., Welsh C. J. R., Nash A. A. 1992; The role of CD8+ T cells in the acute and chronic phases of Theiler′s murine encephalomyelitis virus-induced disease in mice. Journal of General Virology 73:1861–1865
    [Google Scholar]
  6. Brown F. 1995; Antibody recognition and neutralisation of foot-and-mouth disease virus. Seminars in Virology 6:243–248
    [Google Scholar]
  7. Brown F., Graves J. H. 1959; Changes in the specificity and electrophoretic mobility of the precipitating antibodies present in the serum of cattle recovering from foot-and-mouth disease. Nature 183:1688–1691
    [Google Scholar]
  8. Collen T. 1991 T cell responses of cattle to foot-and-mouth disease virus PhD thesis Council for National Academic Awards, UK;
    [Google Scholar]
  9. Collen T. 1994; Foot-and-mouth disease (Aphthovirus): viral T cell epitopes. In Cell-MediatedImmunity in Ruminants pp 173–212 Edited by Goddeevis B. M. L., Morrison I. Boca Raton: CRC Press;
    [Google Scholar]
  10. Collen T., Baron J., Childerstone A., Corteyn A., Doel T. R., Flint M., Garcia-Valcarcel M., Parkhouse R. M. E., Ryan M. D. 1998; Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: the polymerase (P3Dpol) as an immunodominant T-cell immunogen. Virus Research 56:125–133
    [Google Scholar]
  11. DiMarchi R., Brooke G., Gale C., Cracknell V., Doel T. R., Mowat N. 1986; Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science 232:639–641
    [Google Scholar]
  12. Doel T. R., Fletton B. W., Staple R. F. 1982; Qualitative assessment of 146S particles of FMDV in preparations destined for vaccines. Developments in Biological Standardization 50:69–81
    [Google Scholar]
  13. Doherty P. C., Allan W., Eichelberger M., Carding S. R. 1992; Roles of αβ and γδ T cell subsets in viral immunity. Annual Review of Immunology 10:123–151
    [Google Scholar]
  14. Eugui E. M., Emery D. L. 1981; Genetically restricted cell-mediated cytotoxicity in cattle immune to Theileria parva . Nature 290:251–254
    [Google Scholar]
  15. Foster-Cuevas M. 1996 Immunodeterminants of foot-and-mouth disease virus PhD thesis Council for National Academic Awards, UK;
    [Google Scholar]
  16. Garcia-Valcarcel M., Doel T., Collen T., Ryan M., Parkhouse R. M. E. 1996; Recognition of foot-and-mouth disease virus and its capsid protein VP1 by bovine peripheral T lymphocytes. Journal of General Virology 77:727–735
    [Google Scholar]
  17. Glass E. J., Millar P. 1994; Induction of effective cross–reactive immunity by FMDV peptides is critically dependent upon specific MHC-peptide-T cell interactions. Immunology 82:1–8
    [Google Scholar]
  18. Howard C. J., Morrison W. I., Bensaid A., Davis W., Eskra L., Gerdes J., Hadam M., Hurley D., Leibold W., Letesson J.-J., MacHugh N., Naessens J., O’Rielly K., Parsons K. R., Scholte D., Sopp P., Splitter G., Wilson R. 1991; Summary of workshop findings for leukocyte antigens of cattle. Veterinary Immunology and Immunopathology 27:21–27
    [Google Scholar]
  19. McCullough K., De Simone F., Brocchi E., Capucci L., Crowther J. R., Kihm U. 1992; Protective immune response against foot-and-mouth disease. Journal of Virology 66:1835–1840
    [Google Scholar]
  20. Mackowiak C., Lang C., Fontaine J., Camand R., Petermann H. G. 1962; Relationship between neutralising antibody titre and protection in animals immunised against foot-and-mouth disease. Annals de l’Institut Pasteur 103:252–261
    [Google Scholar]
  21. Naessens J., Olubayo R. O., Davis W. C., Hopkins J. 1993; Crossreactivity of workshop antibodies with cells from domestic and wild ruminants. Veterinary Immunology and Immunopathology 39:283–290
    [Google Scholar]
  22. Splitter G. A., Eskra L., Abruzzini A. F. 1988; Cloned bovine cytolytic T cells recognize bovine herpes virus-1 in a genetically restricted, antigen-specific manner. Immunology 63:145–150
    [Google Scholar]
  23. Strohmaier K., Franze R., Adam K.-H. 1982; Location and characterization of the antigenic portion of the FMDV immunizing protein. Journal of General Virology 59:295–306
    [Google Scholar]
  24. Usherwood E. J., Nash A. A. 1995; Lymphocyte recognition of picornaviruses. Journal of General Virology 76:499–508
    [Google Scholar]
  25. Van Lierop M. J. C., van Maanen K., Meloen R. H., Rutten V. P. M. G., de Jong M. A. C., Hensen E. J. 1992; Proliferative lymphocyte responses to foot-and-mouth disease virus and three FMDV peptides after vaccination or immunization with these peptides in cattle. Immunology 75:406–413
    [Google Scholar]
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