1887

Abstract

A novel human papillomavirus (HPV) type, HLT7474-S, was isolated from a cervical scraping of a female sex worker with a wart virus infection. The complete DNA sequence of 7812 bp was derived from four overlapping PCR products and authenticated by RFLP analysis. The L1 gene exhibited 78% identity to those of its most closely related known HPV types in group A7, comprising HPV types 18, 39, 45, 59, 68 and 70. The genomic organization and phylogenetic analysis of HLT7474-S and group A7 HPVs reiterated their relatedness. Of significance were the strong sequence similarity, phylogenetic relationship and conservation of critical motifs between the transforming E6 and E7 of HLT7474-S and E6 of HPV-18 and E7 of HPV-59, respectively. These features clearly suggest that HLT7474-S is a high-risk genital HPV isolate, closely related to HPV-18 and other members of the A7 group of genital HPVs.

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1999-11-01
2020-01-27
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References

  1. Bauer, H. M. , Ting, Y. , Greer, C. E. , Chambers, J. C. , Tashiro, C. J. , Chimera, J. , Reingold, A. & Manos, M. M. ( 1991; ). Genital human papillomavirus infection in female university students as determined by a PCR-based method. Journal of the American Medical Association 265, 472-477.[CrossRef]
    [Google Scholar]
  2. Burger, R. A. , Monk, B. J. , Kurosaki, T. , Anton-Culver, H. , Vasilev, S. A. , Berman, M. L. & Wilczynski, S. P. ( 1996; ). Human papillomavirus type 18: association with poor prognosis in early stage cervical cancer. Journal of the National Cancer Institute 88, 1361-1368 .[CrossRef]
    [Google Scholar]
  3. Chan, S. Y. , Ho, L. , Ong, C. K. , Chow, V. , Drescher, B. , Durst, M. , ter Meulen, J. , Villa, L. , Luande, J. , Mgaya, H. N. & Bernard, H. U. ( 1992; ). Molecular variants of human papillomavirus type 16 from four continents suggest ancient pandemic spread of the virus and its coevolution with humankind. Journal of Virology 66, 2057-2066 .
    [Google Scholar]
  4. Chan, S. Y. , Delius, H. , Halpern, A. L. & Bernard, H. U. ( 1995; ). Analysis of genomic sequences of 95 papillomavirus types: uniting typing, phylogeny, and taxonomy. Journal of Virology 69, 3074-3083 .
    [Google Scholar]
  5. Chow, V. T. K. , Tham, K. M. & Bernard, H. U. ( 1990a; ). Thermus aquaticus DNA polymerase-catalysed chain reaction for the detection of human papillomaviruses. Journal of Virological Methods 27, 101-112.[CrossRef]
    [Google Scholar]
  6. Chow, V. , Tham, K. M. , Yeo-Gloss, M. , Lim-Tan, S. K. , Sng, I. , Thirumoorthy, T. & Bernard, H. U. ( 1990b; ). Molecular diagnosis of genital HPV DNA types by polymerase chain reaction and sensitivity- standardized filter in situ hybridization in randomly sampled cohorts of Singapore women. Molecular and Cellular Probes 4, 121-131.[CrossRef]
    [Google Scholar]
  7. Chow, V. T. K. , Chan, Y. C. , Yong, R. , Lee, K. M. , Lim, L. K. , Chung, Y. K. , Lam-Phua, S. G. & Tan, B. T. ( 1998; ). Monitoring of dengue viruses in field-caught Aedes aegypti and Aedes albopictus mosquitoes by a type-specific polymerase chain reaction and cycle sequencing. American Journal of Tropical Medicine and Hygiene 58, 578-586.
    [Google Scholar]
  8. Cole, S. T. & Danos, O. ( 1987; ). Nucleotide sequence and comparative analysis of the human papillomavirus type 18 genome. Phylogeny of papillomaviruses and repeated structure of the E6 and E7 gene products. Journal of Molecular Biology 193, 599-608.[CrossRef]
    [Google Scholar]
  9. Comerford, S. A. , Maika, S. D. , Laimins, L. A. , Messing, A. , Elsasser, H. P. & Hammer, R. E. ( 1995; ). E6 and E7 expression from the HPV 18 LCR: development of genital hyperplasia and neoplasia in transgenic mice. Oncogene 10, 587-597.
    [Google Scholar]
  10. Crook, T. , Tidy, J. A. & Vousden, K. H. ( 1991; ). Degradation of p53 can be targeted by HPV E6 sequences distinct from those required for p53 binding and trans-activation. Cell 67, 547-556.[CrossRef]
    [Google Scholar]
  11. Delius, H. , Saegling, B. , Bergmann, K. , Shamanin, V. & de Villiers, E. M. ( 1998; ). The genomes of three of four novel HPV types, defined by differences of their L1 genes, show high conservation of the E7 gene and the URR. Virology 240, 359-365.[CrossRef]
    [Google Scholar]
  12. Forslund, O. & Hansson, B. G. ( 1996; ). Human papillomavirus type 70 genome cloned from overlapping PCR products: complete nucleotide sequence and genomic organization. Journal of Clinical Microbiology 34, 802-809.
    [Google Scholar]
  13. Gardiol, D. & Banks, L. ( 1998; ). Comparison of human papillomavirus type 18 (HPV-18) E6-mediated degradation of p53 in vitro and in vivo reveals significant differences based on p53 structure and cell type but little difference with respect to mutants of HPV-18 E6. Journal of General Virology 79, 1963-1970 .
    [Google Scholar]
  14. Goldsborough, M. D. , DiSilvestre, D. , Temple, G. F. & Lorincz, A. T. ( 1989; ). Nucleotide sequence of human papillomavirus type 31: a cervical neoplasia-associated virus. Virology 171, 306-311.[CrossRef]
    [Google Scholar]
  15. Ho, L. , Chan, S. Y. , Chow, V. , Chong, T. , Tay, S. K. , Villa, L. L. & Bernard, H. U. ( 1991; ). Sequence variants of human papillomavirus type 16 in clinical samples permit verification and extension of epidemiological studies and construction of a phylogenetic tree. Journal of Clinical Microbiology 29, 1765-1772 .
    [Google Scholar]
  16. Longuet, M. , Beaudenon, S. & Orth, G. ( 1996; ). Two novel genital human papillomavirus (HPV) types, HPV68 and HPV70, related to the potentially oncogenic HPV39. Journal of Clinical Microbiology 34, 738-744.
    [Google Scholar]
  17. Marich, J. E. , Pontsler, A. V. , Rice, S. M. , McGraw, K. A. & Dubensky, T. W. ( 1992; ). The phylogenetic relationship and complete nucleotide sequence of human papillomavirus type 35. Virology 186, 770-776.[CrossRef]
    [Google Scholar]
  18. Meyer, T. , Arndt, R. , Christophers, E. , Beckmann, E. R. , Schroder, S. , Gissmann, L. & Stockfleth, E. ( 1998; ). Association of rare human papillomavirus types with genital premalignant and malignant lesions. Journal of Infectious Diseases 178, 252-255.[CrossRef]
    [Google Scholar]
  19. Munger, K. & Halpern, A. (1997). HPV16 E7: primary structure and biological properties. In Human Papillomaviruses 1997, pp. III 17–36. Edited by G. Myers, F. Sverdrup, C. Baker, A. McBride, K. Munger & H. U. Bernard. Los Alamos, NM: Los Alamos National Laboratory.
  20. O’Connor, M., Chan, S. Y. & Bernard, H. U. (1995). Transcription factor binding sites in the long control region of genital HPVs. In Human Papillomaviruses 1995, pp. III 21–40. Edited by G. Myers, H. U. Bernard, H. Delius, C. Baker, J. Icenogle & A. Halpern. Los Alamos, NM: Los Alamos National Laboratory.
  21. Palefsky, J. M. , Minkoff, H. , Kalish, L. A. , Levine, A. , Sacks, H. S. , Garcia, P. , Young, M. , Melnick, S. , Miotti, P. & Burk, R. ( 1999; ). Cervicovaginal human papillomavirus infection in human immunodeficiency virus-1 (HIV)-positive and high- risk HIV-negative women. Journal of the National Cancer Institute 91, 226-236.[CrossRef]
    [Google Scholar]
  22. Rapp, L. & Chen, J. J. ( 1998; ). The papillomavirus E6 proteins. Biochimica et Biophysica Acta 1378, F1-F19.
    [Google Scholar]
  23. Rho, J. , Roy-Burman, A. , Kim, H. , de Villiers, E. M. , Matsukura, T. & Choe, J. ( 1994; ). Nucleotide sequence and phylogenetic classification of human papillomavirus type 59. Virology 203, 158-161.[CrossRef]
    [Google Scholar]
  24. Tham, K. M. , Chow, V. T. K. , Singh, P. , Tock, E. P. C. , Ching, K. C. , Lim-Tan, S. K. , Sng, I. T. Y. & Bernard, H. U. ( 1991; ). Diagnostic sensitivity of polymerase chain reaction and Southern blot hybridization for the detection of human papillomavirus DNA in biopsy specimens from cervical lesions. American Journal of Clinical Pathology 95, 638-646.
    [Google Scholar]
  25. Volpers, C. & Streeck, R. E. ( 1991; ). Genome organization and nucleotide sequence of human papillomavirus type 39. Virology 181, 419-423.[CrossRef]
    [Google Scholar]
  26. zur Hausen, H. & de Villiers, E. M. ( 1994; ). Human papillomaviruses. Annual Review of Microbiology 48, 427-447.[CrossRef]
    [Google Scholar]
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