1887

Abstract

The 7·8 kb RI-G fragment of multicapsid nucleopolyhedrovirus (RoMNPV), containing the polyhedrin gene, was cloned and sequenced. The sequence of the fragment was 92·3% identical to the sequence of the corresponding region in the (Ac)MNPV genome. A comparison of the RI-G sequence with other MNPV sequences revealed that RoMNPV was most closely related to AcMNPV. However, the predicted amino acid sequence of RoMNPV polyhedrin shared more sequence identity with the polyhedrin of MNPV. In addition, the RoMNPV sequence was almost completely identical (99·9%) to a previously published 6·3 kb sequence of MNPV (AfMNPV). The RI and dIII restriction fragment profiles of RoMNPV and AfMNPV also were nearly identical, with an additional RI band detected in RoMNPV DNA. Bioassays of these viruses with three different hosts (the European corn borer, H übner, the corn earworm, Boddie, and the tobacco budworm, Fabricius) failed to detect any differences in the biological activities of RoMNPV and AfMNPV. These results indicate that RoMNPV and AfMNPV are different isolates of the same virus. The taxonomic relationship of Ro/AfMNPV and AcMNPV is discussed.

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1999-10-01
2019-12-11
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