1887

Abstract

By analogy with glycoprotein H (gH) of herpes simplex virus type 1 (HSV-1) and pseudorabies virus (PRV), gH may also be essential for penetration and cell-to-cell spread of bovine herpes-virus 1 (BHV-1). This was verified with a gH-negative BHV-1 mutant (gH-BHV-1), which replicated normally on gH-expressing cells but was unable to form plaques and infectious progeny on non-complementing cells. The block in entry could be overcome by polyethylene glycol-induced membrane fusion, demonstrating that gH is not essential for egress. Propagation of gH-BHV-1 on cell lines expressing wild-type gH or gH, which complements the function of BHV-1 gD for cell-to-cell spread, indicated that gH is more efficient than wild-type gH in mediating direct spread of BHV-1. This was supported by the plaque sizes induced by rescued gH-BHV-1 that expressed wild-type gH and gH. Infection of cell lines expressing gH of BHV-1, HSV-1 and PRV with gH-BHV-1, HSV-1 and PRV mutants demonstrated that heterologous gH molecules could not complement gH function in penetration or cell-to-cell spread.

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1999-01-01
2022-10-03
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References

  1. Baranowsky E., Dubuisson J., Pastoret P.-P., Thiry E. 1993; Identification of 108 K, 93 K, and 42 K glycoproteins of bovine herpesvirus-1 by monoclonal antibodies. Archives of Virology 133:97–111
    [Google Scholar]
  2. Browne H. M., Bruun B. C., Minson A. C. 1996; Characterization of herpes simplex virus type 1 recombinants with mutations in the cytoplasmic tail of glycoprotein H. Journal of General Virology 77:2569–2573
    [Google Scholar]
  3. Colbère-Garapin F., Herodniceanu F., Kourilsky P., Garapin A. 1981; A new dominant hybrid selective marker for higher eukaryotic cells. Journal of Molecular Biology 150:1–14
    [Google Scholar]
  4. Forrester A. J., Farrell H., Wilkinson G., Davis-Poynter N., Minson A. C. 1992; Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H sequences deleted. Journal of Virology 66:341–348
    [Google Scholar]
  5. Fuller A. O., Santos R., Spear P. 1989; Neutralizing antibodies specific for glycoprotein H of herpes simplex virus permit viral attachment to cells but prevent penetration. Journal of Virology 63:3435–3443
    [Google Scholar]
  6. Fehler F., Herrmann J. M., Saalmuller A., Mettenleiter T. C., Keil G. M. 1992; Glycoprotein IV of bovine herpesvirus 1-expressing cell line complements and rescues a conditionally lethal viral mutant. Journal of Virology 66:831–839
    [Google Scholar]
  7. Gompels U., Minson A. 1986; The properties and sequence of glycoprotein H of herpes simplex virus type 1. Virology 153:230–247
    [Google Scholar]
  8. Hutchinson L., Browne H., Wargent V., Davis-Poynter N., Primorac S., Goldsmith K., Minson A. C., Johnson D. C. 1992; A novel herpes simplex virus glycoprotein gL forms a complex with glycoprotein H (gH) and affects normal folding and surface expression of gH. Journal of Virology 66:2240–2250
    [Google Scholar]
  9. Khattar S. K., van Drunen Little-van den Hurk S., Attah-Poku S. K., Babiuk L. A., Tikoo S. K. 1996; Identification and characterization of bovine herpesvirus-1 (BHV–1) glycoprotein L which is required for proper antigenicity, processing, and transport of BHV-1 glycoprotein gH. Virology 219:66–76
    [Google Scholar]
  10. Klupp B. G., Fuchs W., Weiland E., Mettenleiter T. C. 1997; Pseudorabies virus glycoprotein L is necessary for virus infectivity but dispensable for virion localization of glycoprotein H. Journal of Virology 71:7687–7695
    [Google Scholar]
  11. Mettenleiter T. C. 1994; Initiation and spread of a-herpesvirus infections. Trends in Microbiology 2:2–4
    [Google Scholar]
  12. Mettenleiter T. C., Spear P. G. 1994; Glycoprotein gB (gII) of pseudorabiesvirus can functionally substitute for glycoprotein gB in herpes simplex virus type 1. Journal of Virology 68:500–504
    [Google Scholar]
  13. Miethke A., Keil G. M., Weiland F., Mettenleiter T. C. 1995; Unidirectional complementation between glycoprotein B homologues of pseudorabies virus and bovine herpesvirus 1 is determined by the carboxy-terminal part of the molecule. Journal of General Virology 76:1623–1635
    [Google Scholar]
  14. Mittal S. K., Field H. J. 1989; Analysis of the bovine herpesvirus type 1 thymidine kinase (TK) gene from wild-type virus and TK-deficient mutants. Journal of General Virology 70:901–918
    [Google Scholar]
  15. Peeters B., de Wind N., Broer R., Gielkens A., Moormann R. 1992; Glycoprotein H of pseudorabiesvirus is essential for entry and cell to cell spread of the virus. Journal of Virology 66:3888–3892
    [Google Scholar]
  16. Roop C., Hutchinson L., Johnson D. C. 1993; A mutant herpes simplex virus type 1 unable to express glycoprotein L cannot enter cells, and its particles lack glycoprotein gH. Journal of Virology 67:2285–2297
    [Google Scholar]
  17. Schröder C., Linde G., Fehler F., Keil G. M. 1997; From essential to beneficial: glycoprotein D loses importance for replication of bovine herpesvirus 1 in cell culture. Journal of Virology 71:25–33
    [Google Scholar]
  18. Spear P. G. 1993; Entry of alphaherpesviruses into cells. Seminars in Virology 4:167–180
    [Google Scholar]
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