1887

Abstract

The location of the BcLFI promoter of Epstein-Barr virus (EBV) has been identified by primer extension, which indicates that the 1 site of the BcLFI mRNA is located at nucleotide 137676 of the EBV genome. According to deletion analysis, the region upstream from nucleotide 38 is not essential for transcription of BcLFI. A 23 bp region in the promoter, from nucleotide 38 to 16, was identified as necessary for regulating the expression of BcLFI, i.e. the promoter activity is activated by 12-O-tetradecanoylphorbol 13-acetate but is repressed by phosphonoacetic acid. The results presented also demonstrate that the oriLyt sequence in is essential for enhancing the expression of BcLF1.

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1998-08-01
2022-01-24
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