1887

Abstract

Prunus necrotic ringspot virus (PNRSV) occurs as numerous strains or isolates that vary widely in their pathogenic, biophysical and serological properties. Prior attempts to distinguish pathotypes based upon physical properties have not been successful; our approach was to examine the molecular properties that may distinguish these isolates. The nucleic acid sequence was determined from 1·65 kbp RT-PCR products derived from RNA 3 of seven distinct isolates of PNRSV that differ sero-logically and in pathology on sweet cherry. Sequence comparisons of ORF 3a (putative movement protein) and ORF 3b (coat protein) revealed single nucleotide and amino acid differences with strong correlations to serology and symptom types (pathotypes). Sequence differences between serotypes and pathotypes were also reflected in the overall phylogenetic relationships between the isolates.

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1998-07-01
2022-05-23
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References

  1. Bachman E. J., Scott S. W., Xin G., Vance V. B. 1994; The complete nucleotide sequence of prune dwarf ilarvirus RNA 3: implications for coat protein activation of genome replication in ilarviruses. Virology 201:127–131
    [Google Scholar]
  2. Banerjee N., Wang J.-Y., Zaitlin M. 1995; A single nucleotide change in the coat protein gene of tobacco mosaic virus is involved in the induction of severe chlorosis. Virology 207:234–239
    [Google Scholar]
  3. Berna A. 1995; Involvement of residues within putative α helix motifs in the behavior of the alfalfa and tobacco mosaic virus movement proteins. Phytopathology 85:1441–1448
    [Google Scholar]
  4. Crosslin J. M., Mink G. I. 1992; Biophysical differences among Prunus necrotic ringspot ilarviruses. Phytopathology 82:200–206
    [Google Scholar]
  5. Crosslin J. M., Hammond R. W., Hammerschlag F. A. 1992; Detection of Prunus necrotic ringspot virus serotypes in herbaceous and Prunus hosts with a complementary RNA probe. Plant Disease 76:1132–1136
    [Google Scholar]
  6. Culver J. N., Stubbs G., Dawson W. O. 1994; Structure-function relationship between tobacco mosaic virus coat protein and hypersensitivity in Nicotiana sylvestris . Journal of Molecular Biology 242:130–138
    [Google Scholar]
  7. Dingjan-Versteegh A., van Vloten-Doting L., Jaspars E. M. J. 1972; Alfalfa mosaic virus hybrids constructed by exchanging nucleoprotein components. Virology 49:716–722
    [Google Scholar]
  8. Felsenstein J. 1987; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  9. Gonsalves D., Fulton R. W. 1977; Activation of Prunus necrotic ringspot virus and rose mosaic virus by RNA 4 components of some ilarviruses. Virology 81:398–407
    [Google Scholar]
  10. Guo D., Maiss E., Adam G., Casper R. 1995; Prunus necrotic ringspot ilarvirus : nucleotide sequence of RNA3 and the relationship to other ilarviruses based on coat protein comparison. Journal of General Virology 76:1073–1079
    [Google Scholar]
  11. Hammond R. W., Crosslin J. M. 1995; The complete nucleotide sequence of RNA 3 of a peach isolate of Prunus necrotic ringspot virus. Virology 208:349–353
    [Google Scholar]
  12. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Computer Applications in the Biosciences 8:189–191
    [Google Scholar]
  13. Holt C. A., Hodgson R. A. J., Coker F. A., Beachy R. N., Nelson R. S. 1990; Characterization of the masked strain of tobacco mosaic virus : identification of the region responsible for symptom attenuation by analysis of an infectious cDNA clone. Molecular Plant-Microbe Interactions 3:417–423
    [Google Scholar]
  14. Howell W. E., Mink G. I. 1988; Natural spread of cherry rugose mosaic disease and two Prunus necrotic ringspot virus biotypes in a central Washington sweet cherry orchard. Plant Disease 72:636–640
    [Google Scholar]
  15. Lister R. M., Saksena K. N. 1976; Some properties of Tulare apple mosaic and ILAR viruses suggesting grouping with tobacco streak virus. Virology 70:440–450
    [Google Scholar]
  16. Mink G. I. 1980; Identification of rugose mosaic-diseased cherry trees by enzyme-linked immunosorbent assay. Plant Disease 64:691–694
    [Google Scholar]
  17. Mink G. I. 1983; The possible role of honeybees in long distance spread of Prunus necrotic ringspot virus from California into Washington sweet cherry orchards. In Plant Virus Epidemiology pp 85–91 Plumb R. T., Thrush J. M. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  18. Mink G. I. 1992; Prunus necrotic ringspot virus. In Plant Diseases of International Importance II pp 335–356 Kumer J., Chaube H. S., Singh U. S., Mukhopadhyay A. N. Edited by New York: Prentice Hall;
    [Google Scholar]
  19. Mink G. I., Aichele M. D. 1984; Use of enzyme-linked immunosorbent assay results in efforts to control orchard spread of cherry rugose mosaic disease in Washington. Plant Disease 68:207–210
    [Google Scholar]
  20. Mink G. I., Howell W. E., Cole A., Regev S. 1987; Three serotypes of Prunus necrotic ringspot virus isolated from rugose mosaic-diseased sweet cherry trees in Washington. Plant Disease 71:91–93
    [Google Scholar]
  21. Moreno I. M., Bernall J. J., Garcia de Blas B., Rodriguez-Cerezo E. 1997; The expression level of the 3a movement protein determines differences in severity of symptoms between two strains of tomato aspermy cucumovirus. Molecular Plant--Microbe Interactions 10:171–179
    [Google Scholar]
  22. Neeleman L., van der Kuyl A. C., Bol J. F. 1991; Role of alfalfa mosaic virus coat protein gene in symptom formation. Virology 181:687–693
    [Google Scholar]
  23. Ong C.-A. 1987 Separation and characterization of nucleoprotein components ofPrunus necrotic ringspot virus isolates PhD thesis Washington State University, Pullman, WA, USA:
    [Google Scholar]
  24. Ong C.-A., Mink G. I. 1989; Evaluation of agarose gel electrophoresis for resolving nucleoprotein components of Prunus necrotic ringspot virus. Phytopathology 79:613–619
    [Google Scholar]
  25. Presting G. G., Smith O. P., Brown C. R. 1995; Resistance to potato leafroll virus in potato plants transformed with the coat protein gene or with vector control constructs. Phytopathology 85:436–442
    [Google Scholar]
  26. Scholthof H. B., Scholthof K.-B.G., Jackson A. O. 1995; Identification of tomato bushy stunt virus host-specific symptom determinants by expression of individual genes from a potato virus X vector. Plant Cell 7:1157–1172
    [Google Scholar]
  27. Scott S. W., Ge X. 1995; The complete nucleotide sequence of RNA 3 of citrus leaf rugose and citrus variegation ilarviruses. Journal of General Virology 76:957–963
    [Google Scholar]
  28. Shiel P. J., Alrefai R. H., Domier L. L., Korban S. S., Berger P. H. 1995; The complete nucleotide sequence of apple mosaic virus RNA-3. Archives of Virology 140:1247–1256
    [Google Scholar]
  29. Sleat D. E., Zhang L., Palukaitis P. 1994; Mapping determinants within cucumber mosaic virus and its satellite RNA for the induction of necrosis in tomato plants. Molecular Plant-Microbe Interactions 7:189–195
    [Google Scholar]
  30. Swofford D. L. 1993; PAUP : phylogenetic analysis using parsimony. Version 3·1 Computer program distributed by the Illinois Natural History Survey. Champaign, Illinois:
    [Google Scholar]
  31. van Vloten-Doting L. 1975; Coat protein is required for infectivity of tobacco streak virus : biological equivalence of the coat proteins of tobacco streak and alfalfa mosaic viruses. Virology 65:215–225
    [Google Scholar]
  32. van Vloten-Doting L., Francki R. I. B., Fulton R. W., Kaper J. M., Lane L. C. 1981; Tricornviridae-a proposed family of plant viruses with tripartite, single-stranded RNA genomes. Intervirology 15:198–203
    [Google Scholar]
  33. Yusibov V. M., Loesch-Fries L. S. 1995; N-terminal basic amino acids of alfalfa mosaic virus coat protein involved in the initiation of infection. Virology 208:405–407
    [Google Scholar]
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