1887

Abstract

Culture supernatant harvested from Daudi cells, a lymphoplastoid cell line, after 58 days of infection with the H77 strain of hepatitis C virus (HCV), was inoculated into a chimpanzee. HCV RNA, as detected by RT-PCR, first appeared in the serum and liver 5 and 6 weeks, respectively, after inoculation. Peripheral blood mononuclear cells (PBMC) collected on week 7 were also positive for HCV RNA. The major sequences of hypervariable region 1 (HVR1) of the viral genome recovered from the inoculated chimpanzee were the ones which were the majority in the original H77 inoculum and not those which were in the majority in the culture supernatant. Only the sequence recovered from PBMC was the same as the major one found in the cell culture.

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/content/journal/jgv/10.1099/0022-1317-79-6-1383
1998-06-01
2022-05-26
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References

  1. Feinstone S. M., Alter H. J., Dienes H., Shimizu Y. K., Popper H., Blackmore D., Sly D., London W. T., Purcell R. H. 1981; Non-A, non-B hepatitis in chimpanzees and marmosets. Journal of Infectious Diseases 144:588–598
    [Google Scholar]
  2. Honda Y., Kondo J., Maeda T., Yoshiyama Y., Yamada E., Shimizu Y. K, Shikata T., Ono Y. 1990; Isolation and purification of a nonA, non-B hepatitis associated microtubular aggregate protein. Journal of General Virology 71:1999–2004
    [Google Scholar]
  3. Inchauspe G., Zebedee S., Lee D. H., Sugutani M., Nasoff M., Prince A. M. 1991; Genetic structure of the human prototype strain H of hepatitis C virus: comparison with American and Japanese isolates. Proceedings of the National Academy of Sciences, USA 88:10292–10296
    [Google Scholar]
  4. Lanford R., Chavez E. D., Chisari F. V., Sureau C. 1995; Lack of detection of negative-strand hepatitis C virus RNA in peripheral blood mononuclear cells and other extrahepatic tissue by the highly strand- specific rTth reverse transcriptase PCR. Journal of Virology 69:8079–8083
    [Google Scholar]
  5. Nakajima N., Hijikata M., Yoshikura H., Shimizu Y. K. 1996; Characterization of long-term culture of hepatitis C virus. Journal of Virology 70:3325–3329
    [Google Scholar]
  6. Shimizu Y. K. 1992; Ultrastructural alterations and expression of cytoplasmic antigen 48-1 in hepatocytes in association with hepatitis C virus infection. Microbiology and Immunology 36:911–922
    [Google Scholar]
  7. Shimizu Y. K., Yoshikura H. 1994; Multicycle infection of hepatitis C virus in cell culture and inhibition by alpha and beta interferons. Journal of Virology 68:8406–8408
    [Google Scholar]
  8. Shimizu Y. K., Yoshikura H. 1995; In vitro systems for the detection of hepatitis C virus infection. Viral Hepatitis Reviews 1:59–65
    [Google Scholar]
  9. Shimizu Y. K., Purcell R. H., Alter H. J., London W. T. 1979; Non-A, non-B hepatitis: ultrastructural evidence for two agents in experimentally infected chimpanzees. Science 205:197–200
    [Google Scholar]
  10. Shimizu Y. K., Oomura M., Abe K., Uno M., Yamada E., Ono Y., Shikata T. 1985; Production of antibody associated with non-A, non- B hepatitis in a chimpanzee lymphoblastoid cell line established by in vitro transformation with Epstein-Barr virus. Proceedings of the National Academy of Sciences, USA 82:2138–2142
    [Google Scholar]
  11. Shimizu Y. K., Weiner A. J., Rosenblatt J., Wong D. C., Shapiro M., Popkin T., Houghton M., Alter H. J., Purcell R. H. 1990; Early events in hepatitis VC virus infection of chimpanzees. Proceedings of the National Academy of Sciences, USA 87:6441–6444
    [Google Scholar]
  12. Shimizu Y. K., Purcell R. H., Yoshikura H. 1993; Correlation between the infectivity of hepatitis C virus in vitro and its infectivity in vivo . Proceedings of the National Academy of Sciences, USA 90:6037–6041
    [Google Scholar]
  13. Shimizu Y. K., Feinstone S. M., Kohara M., Purcell R. H., Yoshikura H. 1996; Hepatitis C virus : detection of intracellular virus particles by electron microscopy. Hepatology 23:205–209
    [Google Scholar]
  14. Shimizu Y. K., Igarashi H., Kanematsu T., Fujiwara K., Wong D. C., Purcell R. H., Yoshikura H. 1997; Sequence analysis of the hepatitis C virus genome recovered from serum, liver, and peripheral blood mononuclear cells of infected chimpanzees. Journal of Virology 71:5769–5773
    [Google Scholar]
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