1887

Abstract

Three maize streak virus (MSV) isolates were derived from an MSV population used to assess the response to infection of maize cultivars. Isolate SP1 was obtained from this population through short acquisition and inoculation periods (1 and 5 min, respectively), using a single vector. Isolate SP2 was derived from SP1 after transmission to a wild perennial host on which it was maintained for about 4 years without insect transmission. Isolate N2A, the most pathogenic isolate, was obtained from the initial population after serial passages on almost completely resistant inbred maize lines. The complexity of each isolate was analysed by RFLP analysis and sequencing based on 120 SP1 clones, 36 SP2 clones and 40 N2A clones. All three isolates were composed of different but related clones, consistent with a quasispecies structure. The mutations were distributed throughout the genome. Mutation frequencies, based on all available sequences, were 3·8 × 10 for SP1, 10· 5 × 10 for SP2 and 6· 9 × 10 for N2A. As expected from the bottleneck selection step, the intra-isolate variability of SP1 was relatively low. Comparison between SP1 and SP2 showed that SP1 heterogeneity increased during maintenance on the wild host. Furthermore, the consensus sequences of SP1 and SP2 differed by two non-synonymous substitutions in the complementary sense gene N2A had a relatively low degree of heterogeneity, but was composed of several sub-populations. The results reflect the influence of the mode of selection of MSV isolates on their quasispecies organization, i.e. distribution of variants, and master sequence.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-12-3091
1998-12-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/12/9880027.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-12-3091&mimeType=html&fmt=ahah

References

  1. Adami C., Pooley J., Glomb J., Stecker E., Fazal F., Fleming J. O., Baker S. C. 1995; Evolution of mouse hepatitis virus (MHV) during chronic infection: quasispecies nature of the persisting MHV RNA. Virology 209:337–346
    [Google Scholar]
  2. Al-Kaff N., Covey S. N. 1994; Variation in biological properties of cauliflower mosaic virus clones. Journal of General Virology 75:3137–3145
    [Google Scholar]
  3. Bendahmane M., Schalk H. -J., Gronenborn B. 1995; Identification and characterization of wheat dwarf virus from France using a rapid method for geminivirus DNA preparation. Molecular Plant Pathology 85:1449–1455
    [Google Scholar]
  4. Bevan M. 1984; Binary Agrobacterium vectors for plant transformation. Nucleic Acids Research 12:8711–8721
    [Google Scholar]
  5. Boulton M. l., King D. l., Donson J., Davies J. W. 1991; Point substitutions in a promoter-like region and the V1 gene affect the host range and symptoms of maize streak virus. Virology 183:114–121
    [Google Scholar]
  6. Briddon R. W., Markham P. G. 1995; FamilyGeminiviridaeInVirus Taxonomy . Sixth Report of the International Committee on Taxonomy of Viruses pp 158–165 Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Edited by Vienna & New York: Springer-Verlag;
    [Google Scholar]
  7. Briddon R. W., Lunness P., Chamberlin L. C. L., Markham P. G. 1994; Analysis of the genetic variability of maize streak virus. Virus Genes 9:93–100
    [Google Scholar]
  8. Chenault K. D., Melcher U. 1994; Patterns of nucleotide sequence variation among cauliflower mosaic virus isolates. Biochimie 76:3–8
    [Google Scholar]
  9. Dekker E. L., Pinner M. S., Markham P. G., VanRegenmortel M. H. V. 1988; Characterization of maize streak virus isolates from different plant species by polyclonal and monoclonal antibodies. Journal of General Virology 69:983–990
    [Google Scholar]
  10. Domingo E., Holland J. J. 1994; Mutation rates and rapid evolution of RNA viruses. In The Evolutionary Biology of Viruses pp 161–184 Morse S. S. Edited by New York: Raven Press;
    [Google Scholar]
  11. Domingo E., Escarmis C., Sevilla N., Moya A., Elena S. F., Quer J., Novella I. S., Holland J. J. 1996; Basic concepts in RNA virus evolution. FASEB Journal 10:859–864
    [Google Scholar]
  12. Drake J. W. 1991; A constant rate of spontaneous mutation in DNA- based microbes. Proceedings of the National Academy of Sciences USA 88:7160–7164
    [Google Scholar]
  13. Drake J. W. 1993; Rates of spontaneous mutation among RNA viruses. Proceedings of the National Academy of Sciences USA 90:4171–4175
    [Google Scholar]
  14. Eigen M., McCaskill J., Schuster P. 1988; Molecular quasispecies. Journal of Physical Chemistry 92:6881–6891
    [Google Scholar]
  15. Enomoto N., Kurosaki M., Tanaka Y., Marumo F., Sato C. 1994; Fluctuation of hepatitis C virus quasispecies in persistent infection and interferon treatment revealed by single-strand conformation polymorphism analysis. Journal of General Virology 75:1361–1369
    [Google Scholar]
  16. Hoisington D., Khairallah M., González de León D. 1992 Laboratory Protocols, 2nd edn. Mexico: CIMMYT Applied Molecular Genetics Laboratory;
    [Google Scholar]
  17. Howell S. H. 1984; Physical structure and genetic organisation of the genome of maize streak virus (Kenyan isolate). Nucleic Acids Research 12:7359–7375
    [Google Scholar]
  18. Hughes F. L., Rybicki E. P., vonWechmar M. B. 1992; Genome typing of southern African subgroup 1 geminiviruses. Journal of General Virology 73:1031–1040
    [Google Scholar]
  19. Huynen M. A., Stadler P. F., Fontana W. 1996; Smoothness within ruggedness: the role of neutrality in adaptation. Proceedings of the National Academy of Sciences, USA 93:397–401
    [Google Scholar]
  20. Kearney C. M., Donson J., Jones G. E., Dawson W. O. 1993; Low level of genetic drift in foreign sequences replicating in an RNA virus in plants. Virology 192:11–17
    [Google Scholar]
  21. Kurath G., Dodds A. 1995; Mutation analyses of a molecular cloned satellite tobacco mosaic virus during serial passages in plants : evidence for hotspots of genetic change. RNA 1:491–500
    [Google Scholar]
  22. Kurath G., Palukaitis P. 1989; RNA sequence heterogeneity in natural populations of three satellite RNAs of cucumber mosaic virus. Virology 173:231–240
    [Google Scholar]
  23. Kurath G., Palukaitis P. 1990; Serial passage ofinfectious transcripts of a cucumber mosaic virus satellite RNA clone results in sequence heterogeneity. Virology 176:8–15
    [Google Scholar]
  24. Kurath G., Rey C. M. E., Dodds A. J. 1992; Analysis of genetic heterogeneity within the type strain of satellite tobacco mosaic virus reveals several variants and a strong bias for G to A substitution mutations. Virology 189:233–244
    [Google Scholar]
  25. Lazarowitz S. G. 1987; Infectivity and complete nucleotide sequence of a South African isolate of maize streak virus. Nucleic Acids Research 16:229–249
    [Google Scholar]
  26. Lazarowitz S. G. 1992; Geminiviruses: genome structure and gene function. In CRC Critical Reviews in Plant Sciences pp 327–349 Conger B. V. Edited by Boca Raton, FL: CRC Press;
    [Google Scholar]
  27. Li X. H., Carrington J. C. 1995; Complementation of tobacco etch virus mutants by active RNA polymerase expressed in transgenic cells. Proceedings of the National Academy of Sciences, USA 92:457–461
    [Google Scholar]
  28. Lichtenstein D. L., Issel C. J., Montelaro R. C. 1996; Genome quasispecies associated with the initiation of infection and disease in ponies experimentally infected with equine infectious anemia virus. Journal of Virology 70:3346–3354
    [Google Scholar]
  29. Mullineaux P. M., Donson J., Morris-Krsinich B. A. M., Boulton M. I., Davies J. W. 1984; The nucleotide sequence of maize streak virus DNA. EMBO Journal 3:3063–3068
    [Google Scholar]
  30. Peterschmitt M., Reynaud B., Sommermeyer G., Baudin P. 1991; Characterization of maize streak virus isolates using monoclonal and polyclonal antibodies and by transmission to a few hosts. Plant Disease 75:27–32
    [Google Scholar]
  31. Peterschmitt M., Granier M., Frutos R., Reynaud B. 1996; Infectivity and complete nucleotide sequence of the genome of a genetically distinct strain of maize streak virus from Reunion island. Archives of Virology 141:1637–1650
    [Google Scholar]
  32. Pinner M. S., Markham P. G., Markham R. H., Dekker E. L. 1988; Characterisation of MSV: description of strains; symptoms. Plant Pathology 37:74–87
    [Google Scholar]
  33. Pirone T. P., Blanc S. 1996; Helper-dependent vector transmission of plant viruses. Annual Review of Phytopathology 34:227–247
    [Google Scholar]
  34. Reynaud B., Peterschmitt M. 1992; A study of transmission of maize streak virus byCicadulina mbilausing an enzyme-linked immunosorbent assay. Annals of Applied Biology 121:85–94
    [Google Scholar]
  35. Rodier A., Assié J., Marchand J. L., Hervé Y. 1995; Breeding maize lines for complete and partial resistance to maize streak virus (MSV). Euphytica 81:15–29
    [Google Scholar]
  36. Rodriguez-Cerezo E., GarcÍa-Arenal F. 1989; Genetic heterogeneity of the RNA genome population of the plant virus U5-TMV. Virology 170:418–423
    [Google Scholar]
  37. Stenger D. C., McMahon C. L. 1997; Genotypic diversity of beet curly top virus populations in the Western United States. American Phytopathological Society Monograph Series 87:737–744
    [Google Scholar]
  38. Stenger D. C., Ostrow K. M. 1996; Genetic complexity of a beet curly top virus population used to assess sugar beet cultivar response to infection. Phytopathology 86:929–933
    [Google Scholar]
  39. Thottappilly G., Bosque-Pérez N. A., Rosse H. W. 1993; Viruses and virus diseases of maize in tropical Africa. Plant Pathology 42:494–509
    [Google Scholar]
  40. Wei X., Ghosh S. K., Taylor M. E., Johnson V. A., Emini E. A., Deutsch P., Lifson J. D., Bonhoeffer S., Nowak M. A., Hahn B. H., Saag M. S., Shaw G. M. 1995; Viral dynamics in human immunodeficiency virus type 1 infection. Nature 373:117–122
    [Google Scholar]
  41. Wright E. A., Heckel T., Groenendijk J., Davies J. W., Boulton M. I. 1997; Splicing features in maize streak virus virion- and complementary-sense gene expression. Plant Journal 12:1285–1297
    [Google Scholar]
  42. Xie Q., Suarez-Lopez P., Gutiérrez C. 1995; Identification and analysis of a retinoblastoma binding motif in the replication protein of a plant DNA virus: requirement for efficient viral DNA replication. EMBO Journal 14:4073–4082
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-12-3091
Loading
/content/journal/jgv/10.1099/0022-1317-79-12-3091
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error