1887

Abstract

The capsid protein (CP) of tomato yellow leaf curl virus (TYLCV) is the only known component of the virus coat. Here, we identify TYLCV CP as a singlestranded (ss) DNA binding protein. Purified TYLCV CP bound ssDNA in a highly cooperative and sequence-nonspecific fashion. TYLCV CP-ssDNA complexes were resistant to nucleolytic digestion and remained stable at relatively high salt concentrations. Because TYLCV CP is known to contain an active nuclear targeting signal, we propose that its association with the viral genomic ssDNA mediates TYLCV entry into the host cell nucleus during the infection process.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-11-2829
1998-11-01
2024-12-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/11/9820160.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-11-2829&mimeType=html&fmt=ahah

References

  1. Chase J. W., Williams K. R. 1986; Single-stranded DNA binding proteins required for DNA replication. Annual Review of Biochemistry 55:103–136
    [Google Scholar]
  2. Citovsky V., Knorr D., Schuster G., Zambryski P. 1990; The P30 movement protein of tobacco mosaic virus is a single-strand nucleic acid binding protein. Cell 60:637–647
    [Google Scholar]
  3. Citovsky V., Knorr D., Zambryski P. 1991; Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA-binding protein. Proceedings of the National Academy of Sciences, USA 88:2476–2480
    [Google Scholar]
  4. Cohen S., Antignus Y. 1994; Tomato yellow leaf curl virus, a whitefly-borne geminivirus of tomato. Advances in Disease Vector Research 10:259–288
    [Google Scholar]
  5. Davies J., Stanley J. 1989; Geminivirus genes and vectors. Trends in Genetics 5:77–81
    [Google Scholar]
  6. Gafni Y., Kunik T., Czosnek H., Citovsky V. 1997; Transgenic tomato plants expressing TYLCV capsid protein are resistant to the virus: the role of the nuclear localization signal (NLS) in the resistance. Acta Horticulturae 447:387–391
    [Google Scholar]
  7. Gerace L., Burke B. 1988; Functional organization of the nuclear envelope. Annual Review of Cell Biology 4:355–374
    [Google Scholar]
  8. Goodman R. M. 1981; Geminiviruses. In Handbook of Plant Virus Infections and Comparative Diagnosis pp. 883–910 Kurstak E. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  9. Guralnick B., Thomsen G., Citovsky V. 1996; Transport of DNA into the nuclei of Xenopus oocytes by a modified VirE2 protein of Agrobacterium . Plant Cell 8:363–373
    [Google Scholar]
  10. Kheyr-Pour A., Bendahmane M., Matzeit N., Accotto G. P., Crespi S., Gronenborn B. 1991; Tomato yellow leaf curl virus from Sardinia is a whitefly-transmitted geminivirus. Nucleic Acids Research 19:6763–6769
    [Google Scholar]
  11. Kunik T., Salomon R., Zamir D., Navot N., Zeidan M., Michelson I., Gafni Y., Czosnek H. 1994; Transgenic tomato plants expressing the tomato yellow leaf curl virus capsid protein are resistant to the virus. Bio/Technology 12:500–504
    [Google Scholar]
  12. Kunik T., Palanichelvam K., Czosnek H., Citovsky V., Gafni Y. 1998; Nuclear import of the tomato yellow leaf curl virus (TYLCV) capsid protein in plant and insect cells. Plant Journal 13:393–399
    [Google Scholar]
  13. Lohman T. M., Overman L. B., Datta S. 1986; Salt-dependent changes in the DNA binding cooperativity of Escherichia coli single strand binding protein. Journal of Molecular Biology 187:603–615
    [Google Scholar]
  14. Navot N., Pichersky E., Zeidan M., Zamir D., Czosnek H. 1991; Tomato yellow leaf curl virus : a whitefly-transmitted geminivirus with a single genomic component. Virology 185:151–161
    [Google Scholar]
  15. Noris E., Hidalgo E., Accotto G. P., Moriones E. 1994; High similarity among the tomato yellow leaf curl virus isolates from the West Mediterranean basin: the nucleotide sequence of an infectious clone from Spain. Archives of Virology 135:165–170
    [Google Scholar]
  16. Noueiry A. O., Lucas W. J., Gilbertson R. L. 1994; Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. Cell 76:925–932
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Sanderfoot A. A., Lazarowitz S. G. 1995; Cooperation in viral movement : the geminivirus BL1 movement protein interacts with BR1 and redirects it from the nucleus to the cell periphery. Plant Cell 7:1185–1194
    [Google Scholar]
  19. Sanderfoot A. A., Ingham D. J., Lazarowitz S. G. 1996; A viral movement protein as a nuclear shuttle. The geminivirus BR1 movement protein contains domains essential for interaction with BL1 and nuclear localization. Plant Physiology 110:23–33
    [Google Scholar]
  20. Timmermans M. C. P., Das O. P., Messing J. 1994; Geminiviruses and their uses as extrachromosomal replicons. Annual Review of Plant Physiology and Plant Molecular Biology 45:79–112
    [Google Scholar]
  21. Zupan J., Citovsky V., Zambryski P. 1996; Agrobacterium VirE2 protein mediates nuclear uptake of ssDNA in plant cells. Proceedings of the National Academy of Sciences, USA 93:2392–2397
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-79-11-2829
Loading
/content/journal/jgv/10.1099/0022-1317-79-11-2829
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error