1887

Abstract

cDNA clones of RNA-2 of two isolates of squash mosaic virus (SqMV) were constructed and sequenced, revealing 87 % sequence similarity. In Northern blot hybridization analyses, DNA probes made from these clones defined two SqMV hybridization subgroups. This grouping was verified by reciprocal hybridizations of purified RNA from five SqMV isolates, as probed with cDNA made from a member of each subgroup. Comparison of the RNA-2 sequence among the two SqMV isolates, and the reported sequence of other comoviruses, showed that SqMV constitutes one of four major branches in a phylogenetic tree of the genus. Analysis of the terminal noncoding sequences showed that although potentially similar folding patterns may form, neither nucleotide sequence nor secondary structural elements are highly conserved among comoviruses. translation products from purified RNA-1 of each subgroup (encoding the viral proteases) were found to process the polyprotein generated by translation of purified RNA-2 from either subgroup.

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1998-10-01
2021-11-28
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References

  1. Alvarez M., Campbell R. N. 1978; Transmission and distribution of squash mosaic virus in seeds of cantaloupe. Phytopathology 68:257–263
    [Google Scholar]
  2. Bruening G. 1978; Comovirus group. MI/AAB Descriptions of Plant Viruses199
    [Google Scholar]
  3. Chen X., Bruening G. 1992; Nucleotide sequence and genetic map of cowpea severe mosaic virus RNA 2 and comparisons with RNA 2 of other comoviruses. Virology 187:682–692
    [Google Scholar]
  4. Franssen H., Goldbach R., Broekhuijsen M., Moerman M., van Kammen A. 1982; Expression of middle-component RNA of cowpea mosaic virus : in vitro generation of a precursor to both capsid proteins by bottom-component RNA-encoded protease from infected cells. Journal of Virology 41:8–17
    [Google Scholar]
  5. Freitag J. H. 1956; Beetle transmission, host range, and properties of squash mosaic virus. Phytopathology 46:73–81
    [Google Scholar]
  6. Goldbach R. W., Wellink J. 1996; Comoviruses : molecular biology and replication. In The Plant Viruses 5 Polyhedral Virions and Bipartite RNA Genomes pp 35–76 Harrison B. D., Murant A. F. Edited by New York: Plenum Press;
    [Google Scholar]
  7. Gopo J. M., Frist R. H. 1977; Location of the gene specifying the smaller protein of the cowpea mosaic virus capsid. Virology 79:259–266
    [Google Scholar]
  8. Hiebert E., Purcifull D. E. 1981; Mapping of the two coat protein genes on the middle RNA component of squash mosaic virus (comovirus group). Virology 113:630–636
    [Google Scholar]
  9. Hu J. S., Pang S. Z., Nagpala P. G., Siemieniak D. R., Slightom J. L., Gonsalves D. 1993; The coat protein genes of squash mosaic virus: cloning, sequence analysis, and expression in tobacco protoplasts. Archives of Virology 130:17–31
    [Google Scholar]
  10. Knuhtsen H. K., Nelson M. R. 1968; Identification of two serotypes in squash mosaic virus strains. Phytopathology 58:345–347
    [Google Scholar]
  11. Kräusslich H., Wimmer E. 1988; Viral proteinases. Annual Review of Biochemistry 57:701–754
    [Google Scholar]
  12. Lastra R., Munz K. 1969; Purification and electron microscopy of squash mosaic virus. Phytopathology 59:1429–1435
    [Google Scholar]
  13. MacFarlane S. A., Shanks M., Davies J. W., Zlotnick A., Lomonos-soff G. P. 1991; Analysis of the nucleotide sequence of bean pod mottle virus middle component RNA. Virology 183:405–409
    [Google Scholar]
  14. Nelson M. R., Knuhtsen H. K. 1973; Squash mosaic virus variability: review and serological comparisons of six biotypes. Phytopathology 63:920–926
    [Google Scholar]
  15. Nelson M. R., Matejka J. C., McDonald H. H. 1965; Systemic infection of watermelon by a strain of squash mosaic virus. Phytopathology 55:1362–1364
    [Google Scholar]
  16. Oxelfelt P., Shanks M., Widmark A. K., Lomonossoff G. P. 1992; Identification and characterization of pseudo-recombinants of red clover mottle comovirus. Journal of General Virology 73:2121–2124
    [Google Scholar]
  17. Provvidenti R., Haudenshield J. S. 1996; Squash mosaic. In Compendium of Cucurbit Diseases pp 41–42 Zitter T. A., Hopkins D. L., Thomas C. E. Edited by St Paul: American Phytopathological Society;
    [Google Scholar]
  18. Rohll J. B., Holness C. L., Lomonossoff G. P., Maule A. 1993; 3ʹ-Terminal nucleotide sequences important for the accumulation of cowpea mosaic virus M-RNA. Virology 193:672–679
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Shanks M., Stanley J., Lomonossoff G. P. 1986; The primary structure of red clover mottle virus middle component RNA. Virology 155:697–706
    [Google Scholar]
  21. Shindo N., Brioso P. S. T., Vicente A. C. P., Krengiel R., Felix D. B., Bauw G., Oliveira D. E. D. 1992; Molecular cloning and sequence analysis of an Andean potato mottle virus cDNA clone encoding the major coat protein. Plant Physiology 100:539–541
    [Google Scholar]
  22. Shindo N., Vicente A. C. P., Krengiel R., de Oliveira D. E. 1993; Nucleotide sequence analysis of an Andean potato mottle virus middle component RNA cDNA clone: Comparisons of the encoded proteins with those of other comoviurses. Intervirology 36:169–180
    [Google Scholar]
  23. Swofford D. L. 1993; PAUP, version 3.1. Champaign, IL: Center for Biodiversity, Illinois Natural History Survey;
    [Google Scholar]
  24. Valverde R. A., Fulton J. P. 1996; Comoviruses: identification and diseases caused. In The Plant Viruses 5 Polyhedral Virions and Bipartite RNA Genomes pp 17–33 Harrison B. D., Murant A. F. Edited by New York: Plenum Press;
    [Google Scholar]
  25. van Wezenbeek P., Verver J., Harmsen J., Vos P., van Kammen A. 1983; Primary structure and gene organization of the middle- component RNA of cowpea mosaic virus. EMBO Journal 2:941–946
    [Google Scholar]
  26. Walter A. E., Turner D. H., Kim J., Lyttle M. H., Mueller P., Mathews D. H., Zuker M. 1994; Coaxial stacking of helixes enhances binding of oligoribonucleotides and improves predictions of RNA folding. Proceedings of the National Academy of Sciences, USA 91:9218–9222
    [Google Scholar]
  27. Zuker M. 1989; On finding all suboptimal foldings of an RNA molecule. Science 244:48–52
    [Google Scholar]
  28. Zuker M. 1997; mfold, version 2.3. http://www.ibc.wustledu/∽zuker/
    [Google Scholar]
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