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Volume 79, Issue 1

Disruption of the 5' and 3' splice sites flanking the major latency-associated transcripts of herpes simplex virus type 1: evidence for alternate splicing in lytic and latent infections Free

Abstract

The herpes simplex virus type 1 (HSV-1) latency- associated transcripts (LATs) are the only viral gene products expressed within latently infected neurones. The most abundant (major) LATs consist of twocollinear nuclear polyA RNAsof2 kb and 1·5 kb which it has been suggested represent stable in- trons derived from a less abundant primary transcript (minor LAT). Consistent with this proposition is the identification of consensus splice donor and acceptor sites flanking major LATs which are conserved between HSV types 1 and 2. Here we test the functionality of the predicted splice sites within the context of the virus genome during productive infection and latent infection To this end viruses in which the LAT splicing signals were disrupted by site-directed mutagenesis were con-structed. We report that mutation of the splice acceptor site abrogates 2 kb major LAT generation during productive infection but does not significantly influence major LAT synthesis during neuronal latency. Similarly, mutation of the splice donor site significantly reduces levels of 2 kb major LAT during productive infection but has no detectable effect on the generation of 2 kb major LAT during neuronal latency as assessed by Northern and hybridization analyses of latently infected neuronal tissue. From these data it can be concluded that the proposed splice sites flanking the major LAT region are dispensable for 2 kb major LAT production in neurones latently infected with HSV-1 but constitute functional splicing signals in productively infected non-neuronal cells.

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© Society for General Microbiology 1998
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1998-01-01
2024-04-26
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Disruption of the 5' and 3' splice sites flanking the major latency-associated transcripts of herpes simplex virus type 1: evidence for alternate splicing in lytic and latent infections
J. Gen. Virol. 79, 107 (1998); https://doi.org/10.1099/0022-1317-79-1-107
/content/journal/jgv/10.1099/0022-1317-79-1-107
/content/journal/jgv/10.1099/0022-1317-79-1-107
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