1887

Abstract

The gene coding for the major capsid protein (VP60) from rabbit haemorrhagic disease virus was expressed in under the phosphoglycerate kinase promoter. The recombinant VP60 produced in yeast was antigenically similar to the viral polypeptide as determined with a polyclonal serum. Electron microscopic observation of the recombinant yeast-derived antigen revealed the presence of virus-like particles similar in size and appearance to native capsids. Subcutaneous vaccination of rabbits with a single dose of this antigen in the absence of commercial adjuvants conferred complete protection against the haemorrhagic disease.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-9-2315
1997-09-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/9/9292020.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-9-2315&mimeType=html&fmt=ahah

References

  1. Argüello Villares J. L. 1991; Viral haemorrhagic disease virus of rabbits: vaccination and immune response. Revue Scientifique et Technique. Office International des Epizooties 10:471–480
    [Google Scholar]
  2. Boga J. A., Marin M. S., Casais R., Prieto M., Parra F. 1992; In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV). Virus Research 26:33–40
    [Google Scholar]
  3. Boga J. A., Casais R., Marín M. S., Martín-Alonso J. M., Cármenes R. S., Prieto M., Parra F. 1994; Molecular cloning, sequencing and expression in Escherichia coli of the capsid protein gene from rabbit haemorrhagic disease virus (Spanish isolate AST/89). Journal of General Virology 75:2409–2413
    [Google Scholar]
  4. Klebe R. J., Harris J. V., Sharp Z. D., Douglas M. G. 1983; A general method for polyethyleneglycol-induced genetic transformation of bacteria and yeast. Gene 25:333–341
    [Google Scholar]
  5. Laurent S., Vautherot J. F., Madeleine M. F., Le Gall G., Rasschaert D. 1994; Recombinant rabbit haemorrhagic disease virus capsid protein expressed in baculovirus self-assembles into virus-like particles and induces protection. Journal of Virology 68:6794–6798
    [Google Scholar]
  6. Liu S. J., Xue H. P., Pu B. Q., Qian N. H. 1984; A new viral disease in rabbits. Animal Husbandry and Veterinary Medicine 16:253–255
    [Google Scholar]
  7. Marín M. S., Martín-Alonso J. M., Pérez-Ordoyo L. I., Boga J. A., Argüello-Villares J. L., Casais R., Venugopal K., Jiang W., Gould E. A., Parra F. 1995; Immunogenic properties of rabbit haemorrhagic disease virus structural protein VP60 expressed by a recombinant baculovirus: an efficient vaccine. Virus Research 39:119–128
    [Google Scholar]
  8. Martín-Alonso J. M., Casais R., Boga J. A., Parra F. 1996; Processing of rabbit haemorrhagic disease virus polyprotein. Journal of Virology 70:1261–1265
    [Google Scholar]
  9. Mellor J., Dobson M. J., Roberts N. A., Tuite M. F., Emtage J. S., White S., Lowe P. A., Patel T., Kingsman A. J., Kingsman S. M. 1983; Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae. Gene 24:1–14
    [Google Scholar]
  10. Meyers G., Wirblich C., Thiel H. J. 1991; Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles. Virology 184:677–686
    [Google Scholar]
  11. Ohlinger V. F., Haas B., Meyers G., Thiel H. J. 1990; Identification and characterization of the virus causing rabbit hemorrhagic disease. Journal of Virology 64:3331–3336
    [Google Scholar]
  12. Parra F., Prieto M. 1990; Purification of a calicivirus as the causative agent of a lethal hemorrhagic disease in rabbits. Journal of Virology 64:4013–4015
    [Google Scholar]
  13. Parra F., Boga J. A., Marín M. S., Casais R. 1993; The aminoterminal sequence of VP60 from rabbit hemorrhagic disease virus supports its putative subgenomic origin. Virus Research 27:219–228
    [Google Scholar]
  14. Romanos M. A., Scorer C. A., Clare J. J. 1992; Foreign gene expression in yeast: a review. Yeast 8:423–488
    [Google Scholar]
  15. Sibilia M., Boniotti M. B., Angoscini P., Capucci L., Rossi C. 1995; Two independent pathways of expression lead to self-assembly of the rabbit hemorrhagic disease virus capsid protein. Journal of Virology 69:5812–5815
    [Google Scholar]
  16. Valenzuela P., Medina A., Rutter W. J., Ammerer G., Hall B. D. 1982; Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature 298:347–350
    [Google Scholar]
  17. Wirblich C., Thiel H. J., Meyers G. 1996; Genetic map of the calicivirus rabbit haemorrhagic disease virus as deduced from in vitro translation studies. Journal of Virology 70:7974–7983
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-9-2315
Loading
/content/journal/jgv/10.1099/0022-1317-78-9-2315
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error