1887

Abstract

The nucleotide sequence of the gene encoding glycoprotein B (gB) of rhesus cytomegalovirus (RhCMV) was determined and the protein characterized. The open reading frame of gB encoded a protein of 854 amino acids with 60% identity and 75 % similarity at the amino acid level to human cytomegalovirus (HCMV) gB. Cysteine residues in the extraluminal part of the protein are perfectly conserved. Out of the 16 potential -linked glyco- sylation sites present in HCMV gB, 15 are conserved in RhCMV gB. Immunoblot analyses with antisera detected three bands of 150 kDa, 90–110 kDa and 55 kDa representing the full-length gB as well as the proteolytic cleavage products. Cross-reactivity and cross-neutralization of a number of HCMV gB-specific monoclonal antibodies with RhCMV gB indicated sharing of immunogenic epitopes between the two molecules. The RhCMV gB regions corresponding to antigenic domains AD-1, 2 and 3 of HCMV gB were immunogenic during natural RhCMV infection with the AD-1 region being the immunodominant domain. The data indicate that RhCMV might represent a useful model to investigate pathogenesis and immune surveillance of cytomegaloviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-8-1999
1997-08-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/8/9267000.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-8-1999&mimeType=html&fmt=ahah

References

  1. Adler S. P., Starr S. E., Plotkin S. A., Hempfling S. H., Buis J., Manning M. L., Best A. M. 1995; Immunity induced by primary human cytomegalovirus infection protects against secondary infection among women of childbearing age. Journal of Infectious Diseases 171:26–32
    [Google Scholar]
  2. Alford C. A., Stagno S., Pass R. F., Britt W. J. 1990; Congenital and perinatal cytomegalovirus infections. Reviews of Infectious Diseases 12: Suppl. 7 S745–S753
    [Google Scholar]
  3. Almeida J., Lang D., Talbot P. 1978; Herpesvirus morphology: visualization of a structural subunit. Intervirology 10:318–320
    [Google Scholar]
  4. Basgoz N., Qadri I., Navarro D., Sears A., Lennette E., Youngblom J., Pereira L. 1992; The amino terminus of human cytomegalovirus glycoprotein B contains epitopes that vary among strains. Journal of General Virology 73:983–988
    [Google Scholar]
  5. Baskin G. B. 1987; Disseminated cytomegalovirus infection in immunodeficient rhesus monkeys. American Journal of Pathology 129:345–352
    [Google Scholar]
  6. Britt W. J., Vugler L. G. 1992; Oligomerization of the human cytomegalovirus major envelope glycoprotein complex gB (gp55-116). Journal of Virology 66:6747–6754
    [Google Scholar]
  7. Britt W. J., Vugler L., Butfiloski E. J., Stephens E. B. 1990; Cell surface expression of human cytomegalovirus (HCMV) gp55-116 (gB): use of HCMV-recombinant vaccinia virus-infected cells in analysis of the human neutralizing antibody response. JournalofVirology 64:1079–1085
    [Google Scholar]
  8. Davis M. G., Kenney S. C., Kamine J., Pagano J. S., Huang E. S. 1987; Immediate-early gene region of human cytomegalovirus trans- activates the promoter of human immunodeficiency virus. Proceedings of the National Academy of Sciences, USA 84:8642–8646
    [Google Scholar]
  9. Ehrlich P. H., Moustafa Z. A., Justice J. C., Harfeldt K. E., Ostberg L. 1988; Further characterization of the fate of human monoclonal antibodies in rhesus monkeys. Hybridoma 7:385–395
    [Google Scholar]
  10. Fowler K. B., Stagno S., Pass R. F., Britt W. J., Boll T. J., Alford C. A. 1992; The outcome of congenital cytomegalovirus infection in relation to maternal antibody status. New England Journal ofMedicine 326:663–667
    [Google Scholar]
  11. Harlow E., Lane D. 1988 Antibodies: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Ho M. 1991 Cytomegalovirus Biology & Infection,, 2nd edn. New York: Plenum;
    [Google Scholar]
  13. Jacobson M. A., Mills J. 1988; Serious cytomegalovirus disease in the acquired immunodeficiency syndrome (AIDS). Clinical findings, diagnosis, and treatment. Annals of Internal Medicine 108:585–594
    [Google Scholar]
  14. Jonjic S., Pavic I., Polic B., Crnkovic I., Lucin P., Koszinowski U. H. 1994; Antibodies are not essential for the resolution of primary cytomegalovirus infection but limit dissemination of recurrent virus. Journal of Experimental Medicine 179:1713–1717
    [Google Scholar]
  15. Kaur A., Daniel M. D., Hempel D., Lee-Parritz D., Hirsch M. S., Johnson P. R. 1996; Cytotoxic T-lymphocyte response to cytomegalovirus in normal and simian immunodeficiency virus-infected rhesus macaques. Journal of Virology 70:7725–7733
    [Google Scholar]
  16. Kniess N., Mach M., Fay J., Britt W. J. 1991; Distribution of linear antigenic sites on glycoprotein gp55 of human cytomegalovirus. Journal of Virology 65:138–146
    [Google Scholar]
  17. Marshall G. S., Rabalais G. P., Stout G. G., Waldeyer S. L. 1992; Antibodies to recombinant-derived glycoprotein B after natural human cytomegalovirus infection correlate with neutralizing activity. Journal of Infectious Diseases 165:381–384
    [Google Scholar]
  18. Marshall G. S., Stout G. G., Knights M. E., Rabalais G. P., Ashley R., Miller H., Rossier E. 1994; Ontogeny of glycoprotein gB-specific antibody and neutralizing activity during natural cytomegalovirus infection. Journal of Medical Virology 43:77–83
    [Google Scholar]
  19. Meyer H., Masuho Y., Mach M. 1990; The gp116 ofthe gp58/116 complex of human cytomegalovirus represents the amino-terminal part of the precursor molecule and contains a neutralizing epitope. Journal of General Virology 71:2443–2450
    [Google Scholar]
  20. Meyer H., Sundqvist V. A., Pereira L., Mach M. 1992; Glycoprotein gp116 of human cytomegalovirus contains epitopes for strain-common and strain-specific antibodies. Journal of General Virology 73:2375–2383
    [Google Scholar]
  21. Nelson J. A., Reynolds-Kohler C., Oldstone M. B., Wiley C. A. 1988; HIV and HCMV coinfect brain cells in patients with AIDS. Virology 165:286–290
    [Google Scholar]
  22. Ohlin M., Sundqvist V. A., Mach M., Wahren B., Borrebaeck C. A. 1993; Fine specificity of the human immune response to the major neutralization epitopes expressed on cytomegalovirus gp58/116 (gB), as determined with human monoclonal antibodies. Journal of Virology 63:703–710
    [Google Scholar]
  23. Rapp M., Messerle M., Buhler B., Tannheimer M., Keil G. M., Koszinowski U. H. 1992; Identification of the murine cytomegalovirus glycoprotein B gene and its expression by recombinant vaccinia virus. Journal of Virology 66:4399–4406
    [Google Scholar]
  24. Reddehase M. J., Mutter W., Munch K., Buhring H. J., Koszinowski U. H. 1987; CD8-positive T lymphocytes specific for murine cytomegalovirus immediate-early antigens mediate protective immunity. Journal of Virology 61:3102–3108
    [Google Scholar]
  25. Reschke M., Reis B., Noding K., Rohsiepe D., Richter A., Mockenhaupt T., Garten W., Radsak K. 1995; Constitutive expression of human cytomegalovirus glycoprotein B (gpUL55) with mutagenized carboxy-terminal hydrophobic domains. Journal of General Virology 76:113–122
    [Google Scholar]
  26. Reusser P., Riddell S. R., Meyers J. D., Greenberg P. D. 1991; Cytotoxic T-lymphocyte response to cytomegalovirus after human allogeneic bone marrow transplantation: pattern of recovery and correlation with cytomegalovirus infection and disease. Blood 78:1373–1380
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Schoppel K., Hassfurther E., Britt W. J., Ohlin M., Borrebaeck C. A., Mach M. 1996; Antibodies specific for the antigenic domain 1 (AD- 1) of glycoprotein B (gpUL55) of human cytomegalovirus bind to different substructures. Virology 216:133–145
    [Google Scholar]
  29. Skolnik P. R., Kosloff B. R., Hirsch M. S. 1988; Bidirectional interactions between human immunodeficiency virus type 1 and cytomegalovirus. Journal of Infectious Diseases 157:508–514
    [Google Scholar]
  30. Vey M., Schafer W., Reis B., Ohuchi R., Britt W., Garten W., Klenk H. D., Radsak K. 1995; Proteolytic processing of human cytomegalovirus glycoprotein B (gpUL55) is mediated by the human endoprotease furin. Virology 206:746–749
    [Google Scholar]
  31. Wagner B., Kropff B., Kalbacher H., Britt W., Sundqvist V. A., Ostberg L., Mach M. 1992; A continuous sequence of more than 70 amino acids is essential for antibody binding to the dominant antigenic site of glycoprotein gp58 of human cytomegalovirus. Journal of Virology 66:5290–5297
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-78-8-1999
Loading
/content/journal/jgv/10.1099/0022-1317-78-8-1999
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error