1887

Abstract

Clones of two subgroup III geminiviruses, the common strain of tomato golden mosaic virus (csTGMV) and African cassava mosaic virus originating from Kenya (ACMV-K), were shown to be non-transmissible by whiteflies. Lack of trans- missibility of cloned ACMV-K was investigated by exchanging genomic components with a whitefly- transmissible ACMV isolate from Nigeria (ACMV- NOg). Neither pseudorecombinant was transmissible, indicating that defects in both genomic components contributed to the lack of trans- missibility. Analysis of the acquisition of the pseudorecombinants by indicated that accumulation of virus within the insect was DNA B dependent. Return of virus to plants was determined by DNA A, although the coat protein was essential for acquisition. Repeated passaging of both the wild strain of ACMV-NOg and the cloned virus led to loss of insect transmissibility of the wild isolate but not the cloned virus. Products encoded on both genomic components are required for transmission of bipartite geminiviruses by .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-7-1791
1997-07-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/7/9225057.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-7-1791&mimeType=html&fmt=ahah

References

  1. Azzam O., Frazer J., de la Rosa D., Beaver J. S., Ahlquist P., Maxwell D. P. 1994; Whitefly transmission and efficient ssDNA accumulation of bean golden mosaic geminivirus requires functional coat protein. Virology 204:289–296
    [Google Scholar]
  2. Bedford I. D., Briddon R. W., Brown J. K., Rosell R. C., Markham P. G. 1994; Geminivirus transmission and biological characterisation of Bemisia tabaci (Gennadius) biotypes from different geographic regions. Annals of Applied Biology 125:311–325
    [Google Scholar]
  3. Bedford I. D., Briddon R. W., Markham P. G., Brown J. K., Rosell R. K. 1992; Bemisia tabaci - biotype characterization and threat of this whitefly species to agriculture. Proceedings of the Brighton Crop Protection Conference - Pests and Diseases 1992:1235–1240
    [Google Scholar]
  4. Boulton M. I., Markham P. G. 1986; The use of squash-blotting to detect plant pathogens in insect vectors. Developments in Applied Biology I. In Developments and Applications in Virus Testing Jones R. A. C., Torrance L. Edited by p. 55–69 Association of Applied Biologists
    [Google Scholar]
  5. Boulton M. I., Steinkellner H., Donson J., Markham P. G., King D. I., Davies J. W. 1989; Mutational analysis of the virion-sense genes of maize streak virus. Journal of General Virology 70:2309–2323
    [Google Scholar]
  6. Briddon R. W., Markham P. G. 1995; Family Geminiviridae. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Edited by Vienna & New York: Springer-Verlag;
    [Google Scholar]
  7. Briddon R. W., Watts J., Markham P. G., Stanley J. 1989; The coat protein of beet curly top virus is essential for infectivity. Virology 172:628–633
    [Google Scholar]
  8. Briddon R. W., Pinner M. S., Stanley J., Markham P. G. 1990; Geminivirus coat protein replacement alters insect specificity. Virology 177:85–94
    [Google Scholar]
  9. Briddon R. W., Bedford I. D., Tsai J. H., Markham P. G. 1996; Analysis of the nucleotide sequence of the treehopper-transmitted geminivirus, tomato pseudo-curly top virus, suggests a recombinant origin. Virology 219:3387–394
    [Google Scholar]
  10. Cohen S., Duffus J. E., Liu H. Y. 1989; Acquisition, interference and retention of cucurbit leaf curl viruses in whiteflies. Phytopathology 79:109–113
    [Google Scholar]
  11. Etessami P., Callis R., Ellwood S., Stanley J. 1988; Delimitation of essential genes of cassava latent virus DNA 2. Nucleic Acids Research 16:4811–4829
    [Google Scholar]
  12. Frischmuth T., Roberts S., von Arnim A., Stanley J. 1993; Specificity of bipartite geminivirus movement proteins. Virology 196:666–673
    [Google Scholar]
  13. Harrison B. D. 1985; Advances in geminivirus research. Annual Review of Phytopathology 23:55–82
    [Google Scholar]
  14. Jeffrey J. L., Pooma W., Petty I. T. D. 1996; Genetic requirements for local and systemic movement of tomato golden mosaic virus in infected plants. Virology 223:208–218
    [Google Scholar]
  15. Klinkenberg F. A., Stanley J. 1990; Encapsidation and spread of African cassava mosaic virus DNA A in the absence of DNA B when agroinoculated to Nicotiana benthamiana. Journal of General Virology 71:1409–1412
    [Google Scholar]
  16. McGrath P. F., Harrison B. D. 1995; Transmission of tomato leaf curl geminiviruses by Bemisia tabaci: effects of virus isolate and vector biotype. Annals of Applied Biology 126:307–316
    [Google Scholar]
  17. Noueiry A. O., Lucas W. J., Gilbertson R. L. 1994; Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. Cell 76:925–932
    [Google Scholar]
  18. Stanley J. 1983; Infectivity of the cloned geminivirus genome requires sequences from both DNAs. Nature 305:643–645
    [Google Scholar]
  19. Stanley J., Gay M. G. 1983; Nucleotide sequence of cassava latent virus DNA. Nature 301:260–262
    [Google Scholar]
  20. Stanley J., Townsend R. 1986; Infectious mutants of cassava latent virus generated in vivo from intact recombinant DNA clones containing single copies of the genome. Nucleic Acids Research 14:5981–5999
    [Google Scholar]
  21. Stanley J., Townsend R., Curson S. J. 1985; Pseudorecombinants between cloned DNAs of two isolates of cassava latent virus. Journal of General Virology 66:1055–1061
    [Google Scholar]
  22. von Arnim A., Stanley J. 1992a; Determinants of tomato golden mosaic virus symptom development located on DNA B. Virology 186:286–293
    [Google Scholar]
  23. von Arnim A., Stanley J. 1992b; Inhibition of African cassava mosaic virus systemic infection by a movement protein from the related geminivirus tomato golden mosaic virus. Virology 187:555–564
    [Google Scholar]
  24. Wu Z. C., Hu J. S., Polston J. E., Ullman D. E., Hiebert E. 1996; Complete nucleotide sequence of a nonvector-transmissible strain of Abutilon mosaic geminivirus in Hawaii. Phytopathology 86:608–613
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-7-1791
Loading
/content/journal/jgv/10.1099/0022-1317-78-7-1791
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error