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Volume 78, Issue 7

Structure and genomic organization of a novel human endogenous retrovirus family: HERV-K (HML-6). Free

Abstract

Prototypic elements of a novel human endogenous retrovirus (HERV) family were identified and cloned from a human genomic library by the use of a fragment, HML-6, related to type A and type B retroviruses and class II HERVs. Out of 39 -hybridizing clones, five contained structures of full-length retroviral proviruses, with regions showing similarity to and , flanked by long terminal repeats (LTRs). Restriction mapping and partial sequence analysis of each full-length clone revealed few conserved restriction sites among HML-6 genomes, and about 20% sequence divergence over the reverse transcriptase region sequenced, suggesting that HML-6 constitutes a heterogeneous, but distinct family of elements belonging to the HERV-K superfamily. Sequence analysis of two clones, HML-6p and HML-6.17, revealed a lysine (K) tRNA UUU primer-binding site, and 40–68% nucleotide sequence similarity to LTR, and regions of type B retroviruses and class II HERVs. HERV-K (HML-6) elements are present at about 30–40 copies per haploid genome. The HML-6 LTRs contain putative progesterone-responsive elements, which may be involved in the regulation of HML-6 expression. Furthermore, there are about 50 additional solitary HML-6 LTRs per haploid genome. Such LTRs were integrated within the region of two clones belonging to the same HML-6 family, indicating that some site preference may be involved in HERV integration.

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© Society for General Microbiology 1997
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1997-07-01
2024-04-26
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Structure and genomic organization of a novel human endogenous retrovirus family: HERV-K (HML-6).
J. Gen. Virol. 78, 1731 (1997); https://doi.org/10.1099/0022-1317-78-7-1731
/content/journal/jgv/10.1099/0022-1317-78-7-1731
/content/journal/jgv/10.1099/0022-1317-78-7-1731
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