1887

Abstract

Recently, we hypothesized that the tumour-suppressive, human helper-virus-dependent, adeno- associated parvoviruses (AAV) may interfere with transforming functions of human papillomaviruses (HPV) in the development of cervical carcinoma. Here, we demonstrate that in cervical epithelium containing papillomavirus DNA, AAV DNA can be detected in a replication-competent form and that AAV proteins are expressed. In cultured cells con-taining integrated AAV-2 DNA, transfection of HPV- 16 DNA induced rescue of infectious AAV-2, revealing helper functions of HPV-16. Similarly, cotransfection of HPV-16 and AAV-2 DNAs into human epithelial cell lines led to replication of AAV-2, and, in keratinocytes, to a cytopathic effect. These data suggest an interaction of the two viruses, possibly influencing the development of HPV-related lesions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-6-1441
1997-06-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/6/9191942.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-6-1441&mimeType=html&fmt=ahah

References

  1. Armbruster E., Tobiasch E., Leiber K., Schlehofer J. R., Rabreau M. 1994; Detection of sequences of adeno-associated virus and human papillomavirus in genital tissue biopsies: a possible interaction ofviruses?. In 2nd International Congress of Papillomavirus in Human Pathology p. 99 Paris: EUROGIN;
    [Google Scholar]
  2. Bantel-Schaal U., Stöhr M. 1992; Influence of adeno-associated virus on adherence and growth properties of normal cells. Journal of Virology 66:773–779
    [Google Scholar]
  3. Bantel-Schaal U., zur Hausen H. 1984; Characterization of the DNA of a defective human parvovirus isolated from a genital site. Virology 134:52–63
    [Google Scholar]
  4. Berns K. I., Bohensky R. A. 1987; Adeno-associated viruses: an update. Advances in Virus Research 32:243–306
    [Google Scholar]
  5. Blacklow N. R., Hoggan M. D., Sereno M. S., Brandt C. D., Kim H. W., Parrot R. H., Chanock R. M. 1971; A seroepidemiologic study of adeno-associated virus infection in infants and children. American Journal of Epidemiology 94:359–366
    [Google Scholar]
  6. Botquin V., Cid-Arregui A., Schlehofer J. R. 1994; Adeno-associated virus type 2 interferes with early development of mouse embryos. Journal of General Virology 75:2655–2662
    [Google Scholar]
  7. Boukamp P., Petrussevska R. T., Breitkreutz D., Hornung J., Markham A., Fusenig N. 1988; Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. Journal of Cell Biology 106:761–771
    [Google Scholar]
  8. Chan W. K., Klock G., Bernard H. U. 1989; Progesterone and glucocorticoid response elements occur in the long control regions of several human papillomaviruses involved in anogenital neoplasia. Journal of Virology 63:3261–3269
    [Google Scholar]
  9. Chan W. K., Chong T., Bernard H. U., Klock G. 1990; Transcription of the transforming genes of the oncogenic human papillomavirus-16 is stimulated by tumor promoters through AP1 binding sites. Nucleic Acids Research 18:736–769
    [Google Scholar]
  10. Crook T., Morgenstern J. P., Crawford L., Banks L. 1989; Continued expression of HPV 16 E7 protein is required for maintenance of the transformed phenotype of cells co-transformed by HPV 16 plus E7-ras. EMBO Journal 8:513–519
    [Google Scholar]
  11. Cukor G., Blacklow N. R., Hoggan D., Berns K. I. 1984; Biology of adeno-associated virus. In The Parvoviruses pp. 33–66 Berns K. I. Edited by New York: Plenum;
    [Google Scholar]
  12. Dürst M., Gissmann L., Ikenberg H., zur Hausen H. 1983; A new papillomavirus DNA from a cervical carcinoma and its prevalence in cancer biopsy samples from different geographic regions. Proceedings of the National Academy of Sciences, USA 80:3812–3815
    [Google Scholar]
  13. Dürst M., Glitz D., Schneider A., zur Hausen H. 1992; Human papillomavirus type 16 (HPV 16) gene expression and DNA replication in cervical neoplasia: analysis by in situ hybridization. Virology 189:132–140
    [Google Scholar]
  14. Feinberg A., Vogelstein B. 1984; A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 137:266–267
    [Google Scholar]
  15. Georg-Fries B., Biederlack S., Wolf J., zur Hausen H. 1984; Analysis of proteins, helper dependence, and seroepidemiology of a new human parvovirus. Virology 134:64–71
    [Google Scholar]
  16. Gloss B., Bernard H. U., Seedorf K., Klock G. 1987; The upstream regulatory region of the human papillomavirus-16 contains an E2 protein-independent enhancer which is specific for cervical carcinoma cells and regulated by glucocorticoids. EMBO Journal 6:3735–3743
    [Google Scholar]
  17. Grossman Z., Mendelson E., Broksimoni F., Mileguir F., Leitner Y., Rechavi G., Ramot B. 1992; Detection of adeno-associated virus type-2 in human peripheral blood cells. Journal of General Virology 73:961–966
    [Google Scholar]
  18. Han L., Parmley T. H., Keith S., Kozlowski K. J., Smith L. J., Hermonat P. L. 1996; High prevalence of adeno-associated virus (AAV) type 2 rep DNA in cervical materials : AAV may be sexually transmitted. Virus Genes 12:47–52
    [Google Scholar]
  19. Heilbronn R., Bürkle A., Stephan S., zur Hausen H. 1990; The adeno-associated virus rep gene suppresses herpes simplex virus-induced DNA amplification. Journal of Virology 64:3012–3018
    [Google Scholar]
  20. Hermonat P. L. 1989; The adeno-associated virus Rep78 gene inhibits cellular transformation induced by bovine papillomavirus. Virology 172:253–261
    [Google Scholar]
  21. Hermonat P. L. 1994; Adeno-associated virus inhibits human papillo-mavirus type 16 : a viral interaction implicated in cervical cancer. Cancer Research 54:2278–2281
    [Google Scholar]
  22. Hörer M., Weger S., Butz K., Hoppe-Seyler F., Geisen C., Kleinschmidt J. A. 1995; Mutational analysis of adeno-associated virus rep protein-mediated inhibition of heterologous and homologous promoters. Journal of Virology 69:5485–5496
    [Google Scholar]
  23. Klein-Bauernschmitt P., zur Hausen H., Schlehofer J. R. 1992; Induction of differentiation-associated changes in established human cells by infection with adeno-associated virus type-2. Journal of Virology 66:4191–4200
    [Google Scholar]
  24. Kotin R. M., Siniscalco M., Samulski R. J., Zhu X. D., Hunter L., Laughlin C. A., McLaughlin S., Muzyczka N., Rocchi M., Berns K. I. 1990; Site-specific integration by adeno-associated virus. Proceedings of the National Academy of Sciences, USA 87:2211–2215
    [Google Scholar]
  25. Laughlin C. A., Tratschin J.-D., Coon H., Carter B. J. 1983; Cloning of infectious adeno-associated virus genome in bacterial plasmids. Gene 23:65
    [Google Scholar]
  26. Mayor H. D. 1993; Defective parvoviruses may be good for your health. Progress in Medical Virology 40:193–205
    [Google Scholar]
  27. Mayor H. D., Drake S., Stahmann J., Mumford D. M. 1976; Antibodies to adeno-associated satellite virus and herpes simplex in sera from cancer patients and normal adults. American Journal of Obstetrics and Gynecology 126:100–104
    [Google Scholar]
  28. Meisels A., Morin C., Casas-Cordero M., Rabreau M. 1983; Human papillomavirus (HPV) veneral infections and gynecologic cancer. Pathology Annual 18:277–293
    [Google Scholar]
  29. Münger K., Phelps W. C., Buttob V., Howley P. M., Schlegel R. 1989; The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Journal of Virology 63:4417–4421
    [Google Scholar]
  30. Muñoz N., Bosch F. X., de Sanjosé S., Shah K. V. 1994; The role of HPV in the etiology of cervical cancer. Mutation Research 305:293–301
    [Google Scholar]
  31. Rabreau M., Schlehofer J. R. 1995; Antagonism of two virus infections in the development of cervical cancer?. Oncology Reports 2:95–97
    [Google Scholar]
  32. Rigby P. W., Rhodes C., Berg P. 1977; Labelling desoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–252
    [Google Scholar]
  33. Ruffing M., Zentgraf H., Kleinschmidt J. A. 1992; Assembly of viruslike particles by recombinant structural proteins of adeno-associated virus type 2 in insect cells. Journal of Virology 66:6922–6930
    [Google Scholar]
  34. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Samulski R. J. 1993; Adeno-associated virus: integration at a specific chromosomal locus. Current Opinion in Geneticsand Development 3:74–80
    [Google Scholar]
  36. Samulski R. J., Zhu X., Xiao X., Brook J. D., Housman D. E., Epstein N., Hunter L. A. 1991; Targeted integration of adeno-associated virus (AAV) into human chromosome 19. EMBO Journal 10:3941–3950
    [Google Scholar]
  37. Schlehofer J. R. 1994; The tumor suppressive properties of adeno- associated viruses. Mutation Research 305:303–313
    [Google Scholar]
  38. Schlehofer J. R., Heilbronn R. 1990; Infection with adeno-associated virus type 5 inhibits mutagenicity of herpes simplex virus type 1 or 4-nitroquinoline-1-oxide. Mutation Research 244:317–320
    [Google Scholar]
  39. Schlehofer J. R., Gissmann L., Matz B., zur Hausen H. 1983a; Herpes simplex virus-induced amplification of SV40 sequences in transformed Chinese hamster embryo cells. International Journal of Cancer 32:99–103
    [Google Scholar]
  40. Schlehofer J. R., Heilbronn R., Georg-Fries B., zur Hausen H. 1983b; Inhibition of initiator-induced SV40 gene amplification in SV40-transformed Chinese hamster cells by infection with a defective parvovirus. International Journal of Cancer 32:591–595
    [Google Scholar]
  41. Schlehofer J. R., Ehrbar M., zur Hausen H. 1986; Vaccinia virus, herpes simplex virus, and carcinogens induce DNA amplification in a human cell line and support replication of a helpervirus dependent parvovirus. Virology 152:110–117
    [Google Scholar]
  42. Schmitt J., Schlehofer J. R., Mergener K., Gissmann L., zur Hausen H. 1989; Amplification of bovine papillomavirus DNA by N-methyl-N′-nitro-N-nitrosoguanidine, ultraviolet irradiation, or infection with herpes simplex virus. Virology 172:73–81
    [Google Scholar]
  43. Schneider A., Hotz M., Gissmann L. 1987; Increased prevalence of human papillomaviruses in the lower genital tract of pregnant women. International Journal of Cancer 40:198–201
    [Google Scholar]
  44. Schneider-Gädicke A., Schwarz E. 1986; Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO Journal 5:2285–2292
    [Google Scholar]
  45. Schwarz E., Freese U. K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., zur Hausen H. 1985; Structure and transcription of human papillomavirus sequences in cervical cells. Nature 314:111–114
    [Google Scholar]
  46. Stoler M. H., Wolinsky S. M., Whitbeck A., Broker T. R., Chow L. T. 1989; Differentiation linked human papillomavirus types 6 and 11 transcription in genital condylomata revealed by in situ hybridization with message specific RNA probes. Virology 172:331–340
    [Google Scholar]
  47. Sverdrup F., Khan S. A. 1994; Replication of human papillomavirus (HPV) DNAs supported by the HPV type 18 E1 and E2 proteins. Journal of Virology 68:505–509
    [Google Scholar]
  48. Swift F. V., Bhat K., Younghusband H. B., Hamada H. 1987; Characterization of a cell type-specific enhancer found in the human papillomavirus 18 genome. EMBO Journal 6:1339–1344
    [Google Scholar]
  49. Tobiasch E., Rabreau M., Geletneky K., Laruë-Charlus S., Severin F., Schlehofer J. R. 1994; Detection of adeno-associated virus DNA in human genital tissue and in material from spontaneous abortion. Journal of Medical Virology 44:215–222
    [Google Scholar]
  50. Walz C., Schlehofer J. R. 1992; Modification of some biological properties of HeLa cells containing adeno-associated virus DNA integrated into chromosome-17. Journal of Virology 66:2990–3002
    [Google Scholar]
  51. Walz C., Schlehofer J. R., Flentje M., Rudat V., zur Hausen H. 1992; Adeno-associated virus sensitizes HeLa cell tumors to gamma-rays. Journal of Virology 66:5651–5657
    [Google Scholar]
  52. Winocour E., Callaham M. F., Huberman E. 1988; Perturbation of the cell cycle by adeno-associated virus. Virology 167:393–399
    [Google Scholar]
  53. Winocour E., Puzis L., Etkin S., Koch T., Danovitch B., Mendelson E., Shaulian E., Karby S., Lavi S. 1992; Modulation of the cellular phenotype by integrated adeno-associated virus. Virology 190:316–329
    [Google Scholar]
  54. Yakobson B., Koch T., Winocour E. 1987; Replication of adeno-associated virus in synchronized cells without the addition of a helper virus. Journal of Virology 61:972–981
    [Google Scholar]
  55. zur Hausen H. 1987; The role of papillomaviruses in human anogenital cancer. In The Papovaviridae pp. 245–259 Salzman P. H. N. P. Edited by New York: Plenum Press;
    [Google Scholar]
  56. zur Hausen H. 1991; Human papillomaviruses in the pathogenesis of anogenital cancer. Virology 184:9–13
    [Google Scholar]
  57. zur Hausen H. 1994; Molecular pathogenesis of cancer of the cervix and its causation by specific HPV types. Current Topics in Microbiology and Immunology 186:131–156
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-6-1441
Loading
/content/journal/jgv/10.1099/0022-1317-78-6-1441
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error