1887

Abstract

Herpesvirus saimiri strain C488 transforms human CD4 T-lymphocytes to continuous interleukin- 2-dependent growth. Unlike human T-cell lines derived from tumours or those transformed by human T-lymphotropic virus 1, herpesvirus saimiri- immortalized T-cells (HVS T-cells) retain many functions of primary activated T-lymphocytes. We have characterized the course of human immunodeficiency virus types 1 and 2 (HIV-1/-2) infection in three HVS T-cell lines. Our results confirm that HVS T-cells are highly permissive to both HIV-1/-2 prototype viruses and to poorly replicating HIV-2 strains of restricted cell tropism. However, the infection was persistently productive for up to 5 months. The down-regulation of surface CD4 molecules was delayed and virus yields significantly exceeded those obtained in T-cell lines.

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/content/journal/jgv/10.1099/0022-1317-78-6-1405
1997-06-01
2022-05-28
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References

  1. Asjö B., Albert J., Chiodi F., Fenyö E. M. 1988; Improved tissue culture technique for production of poorly replicating human immunodeficiency virus strains. Journal of Virological Methods 19:191–196
    [Google Scholar]
  2. Benson R. E., Sanfridson A., Ottinger J. S., Doyle C., Cullen B. R. 1993; Downregulation of cell-surface CD4 expression by Simian immunodeficiency virus Nef prevents viral super infection. Journal of Experimental Medicine 177:1561–1566
    [Google Scholar]
  3. Biesinger B., Müller-Fleckenstein I., Simmer B., Lang G., Wittmann S., Platzer E., Desrosiers R., Fleckenstein B. 1992; Stable growth transformation of human T lymphocytes by Herpesvirus saimiri. Proceedings of the National Academy of Sciences, USA 89:316–399
    [Google Scholar]
  4. Biesinger B., Tsygankov A. Y., Fickenscher H., Emmrich F., Fleckenstein B., Bolen B., Bröker B. M. 1995; The product of the Herpesvirus saimiri open reading frame 1 (Tip) interacts with T-cell specific kinase p56ick in transformed cells. Journal of Biological Chemistry 270:4729–4734
    [Google Scholar]
  5. Bour S., Geleziunas R., Wainberg M. A. 1994; The role ofCD4 and its down-modulation in establishment and maintenance of HIV-1 infection. Immunological Reviews 140:147–171
    [Google Scholar]
  6. Chen B. K., Gandhi R. T., Baltimore D. 1996; CD4 down-modulation during infection of human T-cells with human immunodeficiency virus type-1 involves independent activities of vpu, env and nef. Journal of Virology 70:6044–6053
    [Google Scholar]
  7. Cucchiarini M., Cagnon L., Giordanego V., Doglio A., Lefebvre J. C. 1995; Induction by human immunodeficiency viruses types 1 and 2 of degradation of CD4 but not of a CD4 mutant unable to bind viral envelope glycoproteins. Journal of Acquired Immune Deficiency Syndromes and Human Retroviruses 8:427–436
    [Google Scholar]
  8. Dalgleish A. G., Beverley P. C. L., Clapham P. R., Crawford D. H., Greaves M. F., Weiss R. 1984; The CD4 (T4) antigen is an essential component ofthe receptor for the AIDS retrovirus. Nature 312:763–767
    [Google Scholar]
  9. Evans L. A., Moreau J., Koudou O., Legg H., Barboza A., Cheng-Mayer C., Levy J. A. 1988; Characterization of a noncytopathic HIV-2 strain with unusual effects on CD4 expression. Science 240:1522–1525
    [Google Scholar]
  10. Fenyö E. M., Morfeldt-Manson L., Chiodi F., Lind B., von Gegerfelt A., Albert J., Olausson E., Asjö B. 1988; Distinct replicative and cytopathic characteristics of human immunodeficiency virus isolates. Journal of Virology 62:4414–4419
    [Google Scholar]
  11. Kanner S. B., Haffar O. K. 1995; HIV-1 down-regulates CD4 costimulation of TCR/CD3-directed tyrosine phosphorylation through CD4/p56ick dissociation. Journal of Immunology 154:2996–3005
    [Google Scholar]
  12. Kong L. I., Lee S.-W., Kappes J. C., Parkin J. S., Decker D., Hexie A., Hahn B. H., Shaw G. M. 1988; West African HIV-2 related human retrovirus with attenuated cytopathicity. Science 240:1525
    [Google Scholar]
  13. Levy J. A., Shimabukuro J. 1985; Recovery of AIDS-associated retroviruses from patients with AIDS or AIDS related conditions and from clinically healthy individuals. Journal of Infectious Diseases 152:734–738
    [Google Scholar]
  14. Lusso P., Cocchi F., Balotta C., Markham A. L., Farci P., Pal R., Gallo R. C., Reitz M. S. 1995; Growth of macrophage tropic and primary human immunodeficiency type 1 (HIV-1) isolates in a unique CD4+ T-cell clone (PM1): failure to downregulate CD4 and to interfere with cell-line tropic HIV-1. Journal of Virology 69:3712–3720
    [Google Scholar]
  15. Mittrücker H. W., Müller-Fleckenstein I., Fleckenstein B., Fleischer B. 1992; CD2-mediated autocrine growth of Herpesvirus saimiri-transformed human T-lymphocytes. Journal of Experimental Medicine 176:909–914
    [Google Scholar]
  16. Nick S., Fickenscher H., Biesinger B., Born G., Jahn G., Fleckenstein B. 1993; Herpes saimiri transformed human T-cell lines: a permissive system for human immunodeficiency viruses. Virology 194:875–877
    [Google Scholar]
  17. Saha K., Sova P., Chao W., Chess L., Volsky J. 1996; Generation of CD4+ and CD8+ T-cell clones from PBLs of HIV-1 infected subjects using Herpesvirus Saimiri. Nature Medicine 2:1272–1275
    [Google Scholar]
  18. Schnittman S. M., Psallidoupoulos M. C., Lane H. C., Thompson L., Baseler M., Massari F., Fox C. H., Salzman N. P., Fauci A. S. 1989; The reservoir for HIV-1 in human peripheral blood is a T-cell that maintains expression of CD4. Science 245:304–308
    [Google Scholar]
  19. Schulz T. F., Whitby D., Hoad J. G., Corrah T., Whittle H., Weiss R. A. 1990; Biological and molecular variability of human immunodeficiency virus type 2 isolates from The Gambia. Journal of Virology 64:5177–5182
    [Google Scholar]
  20. Schwartz S., Felber B. K., Fenyö E. M., Pavlakis G. N. 1989; Rapidly and slowing replicating human immunodeficiency virus type 1 isolates can be distinguished according to target cell tropism in T-cell and monocyte cell lines. Proceedings of the National Academy of Sciences, USA 86:7200–7203
    [Google Scholar]
  21. Spira A., Ho D. D. 1995; Effect of different donor cells on human immunodeficiency virus type 1 replication and selection in vitro . Journal of Virology 69:422–429
    [Google Scholar]
  22. Vella C., Jones D., Smith M. H., Barrett W. Y., Farrar G. H., Daniels R. S. 1995; A molecular and serological study of the envelope gene of the British isolate HIV-1 GB8. Vaccine 13:735–742
    [Google Scholar]
  23. Wiese N. A., Tsygankov A. Y., Klauenberg U., Bolen J., Fleischer B., Bröker B. 1996; Selective activation of T-cell kinase p56Lck by Herpesvirus saimiri protein Tip. Journal of Biological Chemistry 271:847–852
    [Google Scholar]
  24. Williams L., Cloyd M. 1991; Polymorph ic human gene determines differential susceptibility of CD4 lymphocytes to infection by certain HIV-1 isolates. Virology 184:723–728
    [Google Scholar]
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