1887

Abstract

Using polyclonal and monoclonal (MAbs) antibodies to human herpesvirus 7 (HHV-7), we have studied HHV-7-specific polypeptides. Human sera were obtained during the convalescent phase from patients with exanthem subitum due to HHV-7, and at least 16 HHV-7-specific polypeptides with apparent molecular masses of 26–210 kDa were immuno- precipitated. Sera prepared in mice also precipitated at least 17 HHV-7-specific polypeptides with molecular masses of 26–210 kDa. Among them, the most commonly observed antigenic protein had an apparent molecular mass of 52 kDa. Forty-two clones secreting MAbs against HHV-7-specific proteins, as determined by immunofluorescence assays, were established from BALB/c mice immunized with HHV-7-infected cell extracts. Seven MAbs which immunoprecipitated HHV-7-specific polypeptides were further characterized. Two of these, MAbs 5E12 and 5F12, reacted predominantly with glycoproteins of 78 kDa and 85 kDa, respectively, and possessed neutralizing activity. This suggests that there are at least two neutralization-inducing proteins in HHV-7. MAb 16B4 reacted with the major immunogenic protein of 52 kDa. Five of the 42 MAbs also reacted in immunofluorescence assays with HHV-6 antigens to the same degree as to HHV- 7. Two other MAbs, 7C10 and 10F1, recognized an HHV-7 protein of 40 kDa, and only 7C10 cross- reacted with an HHV-6 protein of 45 kDa.

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1997-05-01
2024-12-14
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References

  1. Berneman Z. N., Gallo R. C., Ablashi D. V., Frenkel N., Katsafanas G., Kramarsky B., Brus I. 1992a; Human herpesvirus 7 (HHV-7) strain JI: independent confirmation of HHV-7. Journal of Infectious Diseases 166:690–691
    [Google Scholar]
  2. Berneman Z. N., Ablashi D. V., Li G., Eger-Fletcher M., Reitz M. S. Jr Hung C. L., Brus I., Komaroff A. L., Gallo R. C. 1992b; Human herpesvirus 7 is a T-lymphotropic virus and is related to, but significantly different from, human herpesvirus 6 and human cytomegalovirus. Proceedings of the National Academy of Sciences, USA 89:10552–10556
    [Google Scholar]
  3. Black J. B., Inoue N., Kite-Powell K., Zaki S., Pellett P. E. 1993; Frequent isolation of human herpesvirus 7 from saliva. Virus Research 29:91–98
    [Google Scholar]
  4. Black J. B., Schwarz T. F., Patton J. L., Kite-Powell K., Pellett P. E., Wiersbitzky S., Bruns R., Muller C., Jager G., Stewart J. A. 1996; Evaluation of immunoassays for detection of antibodies to human herpesvirus 7. Clinical and Diagnostic Laboratory Immunology 3:79–83
    [Google Scholar]
  5. Foà-Tomasi L., Avitabile E., Ke L., Campadelli-Fiume G. 1994; Polyvalent and monoclonal antibodies identify major immunogenic proteins specific for human herpesvirus 7-infected cells and have weak cross-reactivity with human herpesvirus 6. Journal of General Virology 75:2719–2727
    [Google Scholar]
  6. Foà-Tomasi L., Fiorilli M. P., Avitabile E., Campadelli-Fiume G. 1996; Identification of an 85 kDa phosphoprotein as an immunodominant protein specific for human herpesvirus 7-infected cells. Journal of General Virology 77:511–518
    [Google Scholar]
  7. Frenkel N., Schirmer E. C., Wyatt L. S., Katsafanas G., Roffman E., Danovich R. M., June C. H. 1990; Isolation of a new herpesvirus from human CD4 + T cells. Proceedings of the NationalAcademy ofSciences, USA 87:748–752
    [Google Scholar]
  8. Furukawa M., Yasukawa M., Yakushijin Y., Fujita S. 1994; Distinct effects of human herpesvirus 6 and human herpesvirus 7 on surface molecule expression and function of CD4 + T cells. Journal of Immunology 152:5768–5775
    [Google Scholar]
  9. Hidaka Y., Liu Y., Yamamoto M., Mori R., Miyazaki C., Kusuhara K., Okada K., Ueda K. 1993; Frequent isolation of human herpesvirus 7 from saliva samples. Journal of Medical Virology 40:343–346
    [Google Scholar]
  10. Lusso P., Secchiero P., Crowley R. W., Garzino-Demo A., Berneman Z. N., Gallo R. C. 1994; CD4 is a critical component of the receptor for human herpesvirus 7: interference with human immunodeficiency virus. Proceedings of the National Academy of Sciences, USA 91:3872–3876
    [Google Scholar]
  11. Mukai T., Isegawa Y., Yamanishi K. 1995; Identification of the major capsid protein gene of human herpesvirus 7. Virus Research 37:55–62
    [Google Scholar]
  12. Nicholas J. 1996; Determination and analysis of the complete nucleotide sequence of human herpesvirus. Journal of Virology 70:5975–5989
    [Google Scholar]
  13. Okuno T., Yamanishi K., Shiraki K., Takahashi M. 1983; Synthesis andprocessing ofglycoproteins ofvaricella-zoster virus (VZV) as studied with monoclonal antibodies to VZV antigens. Virology 129:357–368
    [Google Scholar]
  14. Okuno T., Sao H., Asada H., Shiraki K., Takahashi M., Yamanishi K. 1990; Analysis of a glycoprotein of human herpesvirus 6 (HHV-6) using monoclonal antibodies. Virology 176:625–628
    [Google Scholar]
  15. Okuno T., Shao H., Asada H., Shiraki K., Takahashi M., Yamanishi K. 1992; Analysis ofhuman herpesvirus 6 glycoproteins recognized by monoclonal antibody OHV1. Journal of General Virology 73:443–447
    [Google Scholar]
  16. Secchiro P., Berneman Z. N., Gallo R. C., Lusso P. 1994; Biological and molecular characteristics of human herpesvirus 7: in vitro growth optimization and development of a syncytia inhibition test. Virology 202:506–512
    [Google Scholar]
  17. Tanaka K., Kondo T., Torigoe S., Okada S., Mukai T., Yamanishi K. 1994; Human herpesvirus 7: another causal agent for roseola (exanthem subitum). Journal of Pediatrics 125:1–5
    [Google Scholar]
  18. Tanaka-Taya K., Kondo K., Mukai T., Miyoshi H., Yamamoto Y., Okada S., Yamanishi K. 1996; Seroepidemiological study ofhuman herpesvirus-6 and -7 in children of different ages and detection of these two viruses in throat swabs by polymerase chain reaction. Journal of Medical Virology 48:88–94
    [Google Scholar]
  19. Ueda K., Kusuhara K., Okada K., Miyazaki C., Hidaka Y., Tokugawa K., Yamanishi K. 1994; Primary human herpesvirus 7 infection and exanthema subitum. Pediatric Infectious Disease Journal 13:167–168
    [Google Scholar]
  20. Wyatt L. S., Rodriguez W. J., Balachandran N., Frenkel N. 1991; Human herpesvirus 7: antigenic properties and prevalence in children and adults. Journal of Virology 65:6260–6265
    [Google Scholar]
  21. Wyatt L. S., Frenkel N. 1992; Human herpesvirus 7 is a constitutive inhabitant of adult human saliva. Journal of Virology 66:3206–3209
    [Google Scholar]
  22. Yamanishi K., Okuno T., Shiraki K., Takahashi M., Kondo T., Asano Y., Kurata T. 1988; Identification of human herpesvirus-6 as a causal agent for exanthem subitum. Lancet 1:1065–1067
    [Google Scholar]
  23. Yasukawa M., Yakushijin Y., Furukawa M., Fujita S. 1993; Specificity analysis of human CD4+ T-cell clones directed against human herpesvirus 6 (HHV-6), HHV-7, and human cytomegalovirus. Journal of Virology 67:6259–6264
    [Google Scholar]
  24. Yoshikawa T., Asano Y., Kobayashi I., Nakashima T., Yazaki T., Suga S., Ozaki T., Wyatt L. S., Frenkel N. 1993; Seroepidemiology of human herpesvirus 7 in healthy children and adults in Japan. Journal of Medical Virology 41:319–323
    [Google Scholar]
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