1887

Abstract

We have matched a PCR assay which detects disruptions in the E2 reading frame of human papillomavirus type 16, with RNA hybridization patterns and shown that in 15 out of 16 cervical intraepithelial neoplastic (CIN) III lesions and in 19 out of 19 tumours, the E2 gene is disrupted with no detectable E2 transcripts. Varying levels of E6–E7 transcripts are detected in CIN III lesions, with stronger signals in tumours. The cytokeratin profile of most tumours: cytokeratin 10-, 14- and 19-positive and 4-, 13- and 18negative, is also detected in CIN III lesions. The changes in levels of 2, 1 and 4 integrins, CD44 and E-cadherin occur during the evolution of high-grade CIN lesions. Increases in the levels of expression of CD44 and E6-E7 transcripts, coupled with changes in the cellular localization of the Notch protein, define the transition from CIN III lesions to tumours.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-5-1095
1997-05-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/5/9152428.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-5-1095&mimeType=html&fmt=ahah

References

  1. Artavanis-Tsakonas S., Matsuno K., Fortini M. E. 1995; Notch signalling. Science 268:225–232
    [Google Scholar]
  2. Bartsch D., Boye B., Baust C., zur Hausen H., Schwartz E. 1992; Retinoic acid-mediated repression of human papillomavirus 18 transcription and different ligand regulation of the retinoic acid receptor gene in non-tumorigenic and tumorigenic HeLa hybrid cells. EMBO Journal 11:2283–2292
    [Google Scholar]
  3. Cullen A. P., Reid R., Campion M., Lorincz A. T. 1991; Analysis of the physical state of different human papillomavirus DNAs in intraepithelial and in invasive cervical neoplasm. Journal of Virology 65:606–612
    [Google Scholar]
  4. Daniel B., Mukherjee G., Seshadri L., Vallikad E., Krishna S. 1995; Changes in the physical state and expression of human papillomavirus type 16 in the progression of cervical intraepithelial neoplasia lesions analysed by PCR. Journal of General Virology 76:2589–2593
    [Google Scholar]
  5. Das B. C., Sharma J. K., Gopalakrishna V., Luthra U. K. 1992; Analysis by polymerase chain reaction of the physical state of human papillomavirus type 16 DNA in cervical preneoplastic and neoplastic lesions. Journal of General Virology 73:2327–2336
    [Google Scholar]
  6. Di Luca D., Pilotti S., Stefanon B., Rotola A., Monini P., Tognon M., De Palo G., Rilke F., Cassai E. 1986; Human papillomavirus type 16 DNA in genital tumours: a pathological and molecular analysis. Journal of General Virology 67:583–589
    [Google Scholar]
  7. Dong X. P., Stubenrauch F., Beyer-Finkler E., Pfister E. 1994; Prevalence of deletions of YY1 binding sites in episomal HPV 16 DNA from cervical cancers. International Journal of Cancer 58:803–808
    [Google Scholar]
  8. Dürst M., Kleinheinz A., Hotz M., Gissmann L. 1985; The physical state of human papillomavirus type 16 DNA in benign and malignant genital tumours. Journal of General Virology 66:1515–1522
    [Google Scholar]
  9. Dürst M., Glitz D., Schneider A., zur Hausen H. 1992; HPV 16 gene expression and DNA replication in cervical neoplasia: analysis by in situ hybridisation. Virology 189:132–140
    [Google Scholar]
  10. Ellisen L. W., Bird J., West D. C., Soreng A. L., Reynolds T. C., Smith S. D., Sklar J. 1991; TAN-1 the human homolog of the Drosophila Notch gene, is broken by chromosomal translocations in T lymphoblastic neoplasms. Cell 66:649–661
    [Google Scholar]
  11. Hanahan D., Folkman J. 1996; Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell 86:353–364
    [Google Scholar]
  12. Howley P. M. 1991; Role of human papillomaviruses in human cancer. Cancer Research Suppl 51:5019s–5022s
    [Google Scholar]
  13. Lehn H., Villa L. L., Marziona F., Hilgarth M., Hillemans H.-G., Sauer G. 1988; Physical state and biological activity of human papillomavirus genomes in precancerous lesions of the female genital tract. Journal of General Virology 69:187–196
    [Google Scholar]
  14. McBride A. A., Romanczuk H., Howley P. M. 1991; The papillomavirus E2 regulatory proteins. Journal of Biological Chemistry 266:18411–18414
    [Google Scholar]
  15. Plantefaber L. C., Hynes R. O. 1989; Changes in integrin receptors on oncogenically transformed cells. Cell 56:281–290
    [Google Scholar]
  16. Stanley M. A., Sarkar S. 1994; Genetic changes in cervical carcinoma. Papillomavirus Reports 5:141–147
    [Google Scholar]
  17. Stetler-Stevenson W. G., Aznavoorian S., Liotta L. A. 1993; Tumour cell interactions with the extracellular matrix during invasion and metastasis. Annual Review of Cell Biology 9:541–573
    [Google Scholar]
  18. Zagouras P., Stifani S., Blaumueller C. M., Carcangiu M. L., Artavanis-Tsakonas S. 1995; Alterations in Notch signalling in neoplastic lesions of the human cervix. Proceedings of the National Academy of Sciences, USA 92:6414–6418
    [Google Scholar]
  19. Zhang Z., Vuori K., Wang H.-G., Reed J. C., Ruoslahti E. 1996; Integrin activation by R-ras. Cell 85:61–69
    [Google Scholar]
  20. zur Hausen H. 1991; Human papillomaviruses in the pathogenesis of anogenital cancer. Virology 184:9–13
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-5-1095
Loading
/content/journal/jgv/10.1099/0022-1317-78-5-1095
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error