1887

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Volume 78, Issue 5

Mapping of the target antigens of the rotavirus-specific cytotoxic T cell response. Free

Abstract

The cytotoxicT cell (CTL) response in C57/Bl6 (H-2) mice to rotavirus has been analysed using a cognate set of vaccinia virus recombinants covering the 12 primary gene products of the UKtc strain of bovine rotavirus. The gene products of RNA segments 5 (VP5/NSP-1) and 8 (VP7) both elicited a classic CD8 Class I MHC restricted CTL response. Using L cells transfected with specific Class I MHC loci as targets the VP5/NSP-1 response was found to be restricted at D and the VP7 response at K. Vaccinia virus recombinants expressing VP7 genes from seven G serotypes were used to show that the CTL response to this antigen is completely cross-reactive. By contrast, using the same strategy the CTL response to VP5/NSP-1 was found to be virus strain specific. A vaccinia virus recombinant carrying RNA segment 5 from the deletion mutant P9DA5 was used to localize at least one CTL epitope in VP5/ NSP-1 to the first 150 amino acids of the protein. The expression of a number of fragments of VP7 in vaccinia virus recombinants was used to show that the CTL epitope (amino acids 31–40) previously identified through the use of synthetic peptides is virus serotype specific rather than cross-reactive.

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© Society for General Microbiology Ltd 1997
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1997-05-01
2024-04-26
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Mapping of the target antigens of the rotavirus-specific cytotoxic T cell response.
J. Gen. Virol. 78, 1065 (1997); https://doi.org/10.1099/0022-1317-78-5-1065
/content/journal/jgv/10.1099/0022-1317-78-5-1065
/content/journal/jgv/10.1099/0022-1317-78-5-1065
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