1887

Abstract

Little sequence information is available for human immunodeficiency virus type 1 (HIV-1) genes of African origin. Here we describe 37 new complete genes of 18 AIDS patients from Uganda and showthat hasa high genetic variability. proviral DNA sequences of peripheral blood cells were determined by direct sequencing of PCR products. Only 52% of the deduced Vif amino acids were absolutely conserved; when Vif sequences previously analysed were considered, only 32% of the Vif consensus sequence comprised conserved and as such possibly functionally important motifs. The high inter-individual variability was in contrast to a very low intra-individual variability. One patient carried a gene with a stable C-terminal deletion, but N-terminal truncations were not found in patients’ predominant sequences. The genes analysed comprised subtypes A, D and an A/D mosaic. Phylogenetic analyses additionally showed that HIV-1 in Uganda has spread across the boundaries of ethnic groups.

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1997-02-01
2022-08-19
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