1887

Abstract

Small DNA viruses (adenoviruses, simian virus 40, or human papillomaviruses) induce S-phase progression but prevent cell division to provide precursors for viral DNA replication. Herpes simplex viruses types 1 or 2 (HSV-1 or HSV-2) contain genes which encode DNA-metabolizing enzymes, for example, ribonucleotide reductase, thymidine kinase and dUTPase, suggesting that S-phase factors are not required for an efficient infection. However, several studies indicated that HSV induces some events that occur during cell-cycle progression. To determine if HSV-2 induces S-phase entry, we examined serum-arrested African green monkey kidney cells (CV-1) after infection. Two hours after infection steady-state levels of the S-phase-specific cyclin, cyclin A, increased. S-phase cyclin-dependent kinase activity (CDK2) was stimulated 10-fold 8 h after infection but decreased at 16 or 24 h after infection. Mitotic CDK activity (CDC2) was not activated after infection, in part due to decreases in CDC2 protein levels and inactivation of enzymatic activity resulting from tyrosine phosphorylation of CDC2. Furthermore, CDK4 activity was not dramatically affected by infection. These studies indicate that HSV-2 infection selectively activates CDK2 after infection but cell-cycle progression does not occur. We hypothesize that infection activates certain components of the cell cycle which enhance viral gene expression and DNA replication.

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/content/journal/jgv/10.1099/0022-1317-78-12-3341
1997-12-01
2022-05-23
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References

  1. Beijersbergen R. L., Carlee L., Kerkhoven R. M., Bernards R. 1995; Regulat ion of the retinoblastoma protein-related p107 by G1 cyclin complexes. Genes & Development 9:1340–1353
    [Google Scholar]
  2. Berns K. I. 1990; Parvoviridae and their replication. In Fields Virology, 2nd edn. pp. 1743–1763 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  3. Cai W., Schaffer P. A. 1991; A cellular function can enhance gene expression and plating efficiency of a mutant defective in the gene for IE110, a transactivating protein of herpes simplex type 1. Journal of Virology 65:4078–4090
    [Google Scholar]
  4. Cardoso M. C., Leonhardt H., Nadal-Ginard B. 1993; Reversal of terminal differentiation and control of DNA replication: cyclin A and CDK2 specifically localize at subnuclear sites of DNA replication. Cell 74:979–992
    [Google Scholar]
  5. Chen L. I., Nishinaka T., Kwan K., Kitabayashi I., Yokoyama K., Fu Y.-H. F., Grunwald S., Chiu R. 1994; The retinoblastoma gene product Rb stimulates Sp1-mediated transcription by liberating Sp1 from a negative regulator. Molecular and Cellular Biology 14:4380–4389
    [Google Scholar]
  6. Daksis J. I., Preston C. M. 1992; Herpes simplex virus immediate early gene expression in the absence of transinduction by Vmw65 varies during the cell cycle. Virology 189:196–202
    [Google Scholar]
  7. de Bruyn Kops A., Knipe D. 1988; Formation of DNA replication structures in herpes virus-infected cells requires a viral DNA binding protein. Cell 55:857–868
    [Google Scholar]
  8. Gershey E. L. 1979; Simian virus 40-host cell interaction during lytic infection. Journal of Virology 30:76–83
    [Google Scholar]
  9. Greenberg A. H., Litchfield D. W. 1995; Granzymes and apoptosis: targeting the cell cycle. Current Topics in Microbiology and Immunology 198:95–119
    [Google Scholar]
  10. Gu Y., Rosenblatt J., Morgan D. O. 1992; Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15. EMBO Journal 11:3995–4005
    [Google Scholar]
  11. Guadagno T., Newport J. W. 1996; CDK2 kinase is required for entry into mitosis as a positive regulator of CDC2-cyclin B kinase activity. Cell 84:73–82
    [Google Scholar]
  12. Hanson N., Henderson G., Jones C. 1994; The herpes simplex virus type 2 gene which encoded the large subunit of ribonucleotide reductase has unusual regulatory properties. Virus Research 34:265–280
    [Google Scholar]
  13. Hayward G. S. 1993; Immediate early gene regulation in herpes simplex virus. Seminars in Virology 4:15–23
    [Google Scholar]
  14. He J., Choe S., Walker R., DiMarzio P., Morgan D. O., Landeau N. R. 1995; Human Vpr arrests cells in the G2 phase of the cell cycle by inhibiting p34CDC2 activity. Journal of Virology 69:6705–6711
    [Google Scholar]
  15. Heichman K. A., Roberts J. M. 1994; Rules to replicate by. Cell 79:557–562
    [Google Scholar]
  16. Heilbronn R., zur Hausen H. 1989; A subset ofherpes simplex virus replication genes induces DNA amplification within the host cell genome. Journal of Virology 63:3683–3692
    [Google Scholar]
  17. Hilton J. M., Mounghane D., Mclean T., Contractor N. V., O’Neil J., Carpenter K., Bachenheimer S. 1995; Induction of free and heterotrimeric forms of E2F transcription factor by herpes simplex virus. Virology 213:624–638
    [Google Scholar]
  18. Hossain A., Schang L., Jones C. 1995; Identification of gene products encoded by the latency related gene of bovine herpes virus type 1. Journal of Virology 69:5345–5352
    [Google Scholar]
  19. Kulomaa P., Paavonen J., Lehtinen M. 1992; Herpes simplex virus induces unscheduled DNA synthesis in virus-infected cervical cancer cell lines. Research in Virology 143:351–359
    [Google Scholar]
  20. Liptak L. M., Uprichard S. L., Knipe D. M. 1996; Functional order of assembly of herpes simplex virus DNA replication proteins into prereplicative site structures. Journal of Virology 70:1759–1767
    [Google Scholar]
  21. Lukonis C. J., Weller S. K. 1996; Characterization of nuclear structures in cells infected with herpes simplex virus type 1 in the absence of viral DNA replication. Journal of Virology 70:1751–1758
    [Google Scholar]
  22. Maltzman W., Czyzyk L. 1984; UV irradiation stimulates levels of p53 cellular tumor antigen in nontransformed mouse cells. Molecular and Cellular Biology 4:1689–1694
    [Google Scholar]
  23. Matsushime H., Quelle D. E., Shurtleff S. A., Shibuya M., Sherr C. J., Kato J.-Y. 1994; D-type cyclin-dependent kinase activity in mammalian cells. Molecular and Cellular Biology 14:2066–2076
    [Google Scholar]
  24. Nacht M., Reed S. I., Alwine J. C. 1995; Simian virus 40 large T antigen affects the Saccharomyces cerevisiae cell cycle and interacts with p34CDC28. Journal of Virology 69:756–763
    [Google Scholar]
  25. Norbury C., Blow J., Nurse P. 1991; Regulatory phosphorylation of the p34CDC2 protein kinase in vertebrates. EMBO Journal 10:3321–3329
    [Google Scholar]
  26. Nurse P. 1994; Ordering S phase and M phase in the cell cycle. Cell 79:547–550
    [Google Scholar]
  27. O’Hare P. 1993; The virion transactivator of herpes simplex virus. Seminars in Virology 4:145–155
    [Google Scholar]
  28. Pagano M., Pepperkok P., Ansorge W., Draetta G. 1992; Cyclin A is required at two points in the human cell cycle. EMBO Journal 11:961–971
    [Google Scholar]
  29. Pilon L., Langelier Y., Royal A. 1986; Herpes simplex virus type 2 mutagenesis: characterization of mutants induced at the hprt locus of nonpermissive XC cells. Molecular and Cellular Biology 6:539–557
    [Google Scholar]
  30. Ralph W. M., Cabatingan M. S., Schaffer P. A. 1994; Induction of herpes simplex virus type 1 immediate early gene expression by a cellular activity expressed in Vero and NB41A3 cells after growth arrest release. Journal of Virology 68:6871–6882
    [Google Scholar]
  31. Reinhardt C. G., Krugh T. R. 1978; A comparative study of ethidium bromide complexes with dinucleotides and DNA: direct evidence for intercalation and nucleic acid sequence preferences. Biochemistry 17:4845–4854
    [Google Scholar]
  32. Resnitzky D., Reed S. I. 1995; Different roles for cyclins D1 and E in regulation of the G1-to-S transition. Molecular and Cellular Biology 15:3463–3469
    [Google Scholar]
  33. Resnitzky D., Hengst L., Reed S. I. 1995; Cyclin A-associated kinase activity is rate limiting for entrance into S phase and is negatively regulated in G1 by p27Kip1. Molecular and Cellular Biology 15:4347–4352
    [Google Scholar]
  34. Roizman B., Sears A. E. 1990; Herpes simplex viruses and their replication. In Fields Virology, 2nd edn. pp. 1795–1841 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  35. Scarano F. J., Laffin J. A., Laffin J. M., Lehman J. M., Freidrich T. D. 1994; Simian virus 40 prevents activation of M-phase-promoting factor during lytic infection. Journal of Virology 68:2355–2361
    [Google Scholar]
  36. Schang L., Hossain A., Jones C. 1996; The latency related gene of bovine herpes virus type 1 encodes a factor which inhibits cell cycle progression. Journal of Virology 70:3807–3814
    [Google Scholar]
  37. Schlehofer J. R., zur Hausen H. 1982; Induction of mutations within the host cell genome by partially inactivated HSV-1. Virology 122:471–475
    [Google Scholar]
  38. Sherr C. J. 1994; G1 phase progression: cyclin on cue. Cell 79:551–555
    [Google Scholar]
  39. Shin E. K., Tevosian S. G., Yee A. Y. 1996; The N-terminal region of E2F-1 is required for transcriptional activation of a new class of target promoter. Journal of Biological Chemistry 271:12261–12268
    [Google Scholar]
  40. Wang H., Jones C., Zanella J., Holt T., Gilchrist D., Dickman M. 1996; Fumonisins and Alternaria alternata lycopersici toxins : sphinganine analog mycotoxins induce apoptosis in monkey kidney cells. Proceedings of the National Academy of Sciences, USA 93:3461–3465
    [Google Scholar]
  41. Wang J. K. J., Knudson E. S., Welch P. J. 1994; The retinoblastoma tumor suppressor protein. Advances in Cancer Research 64:25–85
    [Google Scholar]
  42. Weinberg R. A. 1995; The retinoblastoma protein and cell cycle control. Cell 81:323–330
    [Google Scholar]
  43. Wilcock D., Lane D. P. 1991; Localization of p53, retinoblastoma and host replication proteins at sites of viral replication in herpes-infected cells. Nature 349:429–431
    [Google Scholar]
  44. Zarkowska T., Mittnacht S. 1997; Differential phosphorylation of the retinoblastoma protein by G1/S cyclin-dependent kinases. Journal of Biological Chemistry 272:12738–12745
    [Google Scholar]
  45. Zhan Q., Carrier F., Fornace A. J.Jr 1993; Induction of cellular p53 activity by DNA damaging agents and growth arrest. Molecular and Cellular Biology 13:4242–4250
    [Google Scholar]
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