1887

Abstract

The genome structure of a nucleopolyhedrovirus (NPV) isolated from the bertha armyworm, Walker (Lepidoptera: Noctuidae) (MacoNPV) was analysed with six restriction endo-nucleases (REN): HI, RI, dIII, I, I, and I. More than 70 MacoNPV REN fragments were cloned into plasmids pUC18 and pBluescript SK( + ). The physical map with 112 restriction sites for the above REN was constructed using double digests and Southern blot hybridization analysis of the MacoNPV DNA clones. The size of the DNA genome of the MacoNPV-90/2 isolate used for this study was estimated at 156 kbp based on REN fragment sizes. The position of the polyhedrin gene, which has by convention been used as the zero point of the REN maps of NPV, was determined by hybridizing the multicapsid nucleopolyhedrovirus dIII-V fragment clone, which contains most of the polyhedrin gene, with genomic blots of MacoNPV. The cloned MacoNPV fragments identified as containing the polyhedrin gene were sequenced and an ORF coding for a 246 amino acid polypeptide with 98·7% sequence identity with nucleopolyhedrovirus (PaflNPV) polyhedrin protein was identified. The putative polyhedrin gene sequence had 97·2% and 91·2% identity with the PaflNPV and multicapsid nucleopolyhedrovirus polyhedrin gene sequences, respectively, and also contained an upstream region identical to the highly conserved 12 bp consensus sequence TGTAAGTAATTT typical of NPV very late genes.

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1997-01-01
2024-04-26
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References

  1. Bracken G. K., Bucher G. E. 1977; An estimate of the relation between density of bertha armyworm (Mamestra configurata) and yield loss on rapeseed, based on artificial infestations. Journal of Economic Entomology 70:701–705
    [Google Scholar]
  2. Bucher G. E., Bracken G. K. 1976; The bertha armyworm, Mamestra configurata (Lepidoptera: Noctuidae). Artificial diet and rearing technique. Canadian Entomologist 108:1327–1338
    [Google Scholar]
  3. Bucher G. E., Turnock W. J. 1983; Dosage responses of the larval instars of the bertha armyworm, Mamestra configurata (Lepidoptera: Noctuidae), to a native nuclear polyhedrosis. Canadian Entomologist 115:341–349
    [Google Scholar]
  4. Cameron I. R., Possee R. D. 1989; Conservation of polyhedrin gene promoter function between Autographa californica and Mamestra brassicae nuclear polyhedrosis viruses. Virus Research 12:183–200
    [Google Scholar]
  5. Erlandson M. 1990; Biological and biochemical comparison of Mamestra configurata and Mamestra brassicae nuclear polyhedrosis virus isolates pathogenic for the bertha armyworm, Mamestra configurata (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology 56:47–56
    [Google Scholar]
  6. Hooft van Iddekinge B. J. L., Smith G. E., Summers M. D. 1983; Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Virology 131:561–565
    [Google Scholar]
  7. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Oakey R., Cameron I. R., Davis B., Davis E., Possee R. D. 1989; Analysis of transcription initiation in the Panolis flammea nuclear polyhedrosis virus polyhedrin gene. Journal of General Virology 70:769–775
    [Google Scholar]
  9. Possee R. D., Kelly D. C. 1988; Physical maps and comparative DNA hybridization of Mamestra brassicae and Panolis flammea nuclear polyhedrosis virus genomes. Journal of General Virology 69:1285–1298
    [Google Scholar]
  10. Possee R. D., Sun T.-P., Howard S. C., Ayres M. D., Hill-Perkins M., Gearing K. L. 1991; Nucleotide sequence of the Autographa californica nuclear polyhedrosis virus 9·4 kbp Eco RI-I and -R (polyhedrin gene) region. Virology 185:229–241
    [Google Scholar]
  11. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  12. Smith G. E., Summers M. D. 1978; Analysis of baculovirus genomes with restriction endonucleases. Virology 89:517–528
    [Google Scholar]
  13. Smith I. R. L., Crook N. E. 1988; Physical maps of the genomes of four variants of Artogeia rapae granulosis virus. Journal of General Virology 69:1741–1747
    [Google Scholar]
  14. Turnock W. J. 1988; Density, parasitism, and disease incidence of larvae of the bertha armyworm, Mamestra configurata Walker (Lepidoptera: Noctuidae), in Manitoba, 1973-1986. Canadian Entomologist 120:401–413
    [Google Scholar]
  15. Wylie H. G., Bucher G. E. 1977; The bertha armyworm, Mamestra configurata Walker (Lepidoptera: Noctuidae). Mortality of immature stages on the rape crop, 1972-1975. Canadian Entomologist 109:823–837
    [Google Scholar]
  16. Vlak J. M., Smith G. E. 1982; Orientation of the genome of Autographa californica nuclear polyhedrosis virus: a proposal. Journal of Virology 41:1118–1121
    [Google Scholar]
  17. Zanotto P. M. de A., Kessing B. D., Maruniak J. E. 1993; Phylogenetic interrelationships among baculoviruses : evolutionary rates and host associations. Journal of Invertebrate Pathology 62:147–164
    [Google Scholar]
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