1887

Abstract

Very little is known about the factors that determine the outcome of infection by human T cell leukaemia virus type I (HTLV-I) and the neurotropism of this virus is still a controversial point. In transgenic mice, the HTLV-I LTR is active mainly in the central nervous system (CNS), in parenchyma as well as in ependymal and choroid plexus cells. The latter are of particular interest and could represent the way of entry of the virus into the CNS. In this study we show that primary cultures of sheep choroid plexus can be infected with HTLV-I, leading to characteristic multinucleated syncytial cells containing virus RNA and proteins. HTLV-I p24 Gag protein was detected in the culture medium and the presence of virus particles was observed by electron microscopy 40 days after infection. At this time post-infection HTLV-I could be transmitted to human cord blood cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-1-143
1997-01-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/1/9010297.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-1-143&mimeType=html&fmt=ahah

References

  1. Bhigjee A. I., Wiley C. A., Wachsman W., Amenomori T., Pirie D., Bill P. L. A., Windsor I. 1991; clinicopathologic correlation with localization of provirus to spinal cord. Neurology 41:1990–1992
    [Google Scholar]
  2. Brahic M., Ozden S. 1992; Simultaneous detection of cellular RNA and proteins. In In situ Hybridization - A Practical Approach pp 85–104 Wilkinson R. G. Edited by Oxford: IRL Press;
    [Google Scholar]
  3. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  4. Falangola M. F., Hanly A., Galvao-Castro B., Petito C. K. 1995; HIV infection of human choroid plexus : a possible mechanism of viral entry into the CNS. Journal of Neuropathology and Experimental Neurology 54:497–503
    [Google Scholar]
  5. Georgsson G. 1994; Neuropathologic aspects of lentiviral infections. Annals of the New York Academy of Science 724:50–67
    [Google Scholar]
  6. Gessain A., Barin E., Vernant J. C., Gout O., Maurs L., Calender A., de Thé G. 1985; Antibodies to human T-lymphotropic virus type- 1 in patients with tropical spastic paraparesis. Lancet ii:407–410
    [Google Scholar]
  7. Gonzalez-Dunia D., Chirinian-Syan S., Brahic M., Ozden S. 1992; Functional analysis of two long terminal repeats from the HTLV-I retrovirus. Gene 116:151–158
    [Google Scholar]
  8. Gonzalez-Dunia D., Komurian-Pradel F., Chirinian-Syan S., de Thé G., Brahic M., Ozden S. 1993; Comparative analysis of HTLV-I promoter activities reveals no disease-linked pattern of expression. Aids Research and Human Retroviruses 9:337–341
    [Google Scholar]
  9. Gupta A., Mingioli E. S., Mora C., McFarlin D. E. 1988; Detection of anti-IgG, IgM, and IgA antibodies against HTLV-I in sera and CSF of patients with tropical spastic paraparesis (TSP). Neurology 38:89–95
    [Google Scholar]
  10. Hara H., Morita M., Iwaki T., Hatae T., Itoyama Y., Kitamoto T., Akizuki S. I., Goto I., Watanabe T. 1994; Detection of human T lymphotrophic virus type I (HTLV-I) proviral DNA and analysis of T cell receptor Vfi CDR3 sequences in spinal cord lesions of HTLV-I associated myelopathy/tropical spastic paraparesis. Journal of Experimental Medicine 180:831–839
    [Google Scholar]
  11. Hirose S., Uemura Y., Fujishita M., Kitagawa T., Yamashita M., Imamura J., Ohtsuki Y., Taguchi H., Miyoshi I. 1986; Isolation of HTLV-I from cerebrospinal fluid of a patient with myelopathy. Lancet ii:397–398
    [Google Scholar]
  12. Hooghe-Peters E., Dubois-Dalcq M., Schmechel D. 1979; Visna virus-induced fusion of nerve cells in vitro. Laboratory Investigation 41:247–255
    [Google Scholar]
  13. Jacobson S., Raine C. S., Mingioli E. S., McFarlin D. E. 1988; Isolation of an HTLV-I-like retrovirus from patients with tropical spastic paraparesis. Nature 331:540–543
    [Google Scholar]
  14. Jacobson S., Shida H., McFarlin D. E., Fauci A. S., Koenig S. 1990; Circulating CD8 + cytotoxic T lymphocytes specific for HTLV- I pX in patients with HTLV-I associated neurological disease. Nature 348:245–248
    [Google Scholar]
  15. Kira T., Itoyama Y., Koyanagi Y., Tateishi J., Kishikawa M., Akizuki S., Kobayashi I., Toki N., Sueishi K., Sato H., Sakaki Y., Yamamoto N., Goto I. 1992; Presence of HTLV-I proviral DNA in central nervous system of patients with HTLV-I associated myelopathy. Annals of Neurology 31:39–45
    [Google Scholar]
  16. Kubota R., Umehara F., Izumo S., Ijishi S., Matsumuro K., Yashiki S., Fujiyoshi T., Sonoda S., Osame M. 1994; HTLV-I proviral DNA amount correlates with infiltrating CD4 + lymphocytes in the spinal cord from patients with HTLV-I associated myelopathy. Journal of Neuroimmunology 53:23–29
    [Google Scholar]
  17. Lehky T. J., Fox C. H., Koenig S., Levin M. C., Flerlage N., Izumo S., Sato E., Raine C. S., Osame M., Jacobson S. 1995; Detection of human T-lymphotropic virus type I (HTLV-I) Tax RNA in the central nervous system of HTLV-I-associated myelopathy/tropical spastic paraparesis patients by in situ hybridization. Annals of Neurology 37:167–175
    [Google Scholar]
  18. Osame M., Usuku K., Izumo S., Ijichi N., Amitani H., Igata A., Matsumoto M., Tara M. 1986; HTLV-I associated myelopathy, a new clinical entity. Lancet i:1031–1032
    [Google Scholar]
  19. Poiesz B. J., Ruscetti F. W., Gadzar A. F., Bunn P. A., Minna J. D., Gallo R. C. 1980; Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T cell lymphoma. Proceedings of the National Academy of Sciences USA: 777415–7419
    [Google Scholar]
  20. Richardson J. H., Edwards A. J., Cruickshank J. K., Rudge P., Dalgleish A. G. 1990; In vivo cellular tropism of human T-cell leukemia virus type I. Journal of Virology 64:5682–5687
    [Google Scholar]
  21. Stoica G., Illanes O., Tasca S. I., Wong P. K. 1993; Temporal central and peripheral nervous system changes induced by a paralyto- genic mutant of Moloney murine leukemia virus TB. Laboratory Investigation 69:724–735
    [Google Scholar]
  22. Yoshida B., Miyoshi I., Hinuma Y. 1982; Isolation and characterization of retrovirus from cell lines of human adult T cell leukaemia and its implication in the disease. Proceedings of the National Academy of Sciences USA: 792031–2035
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-78-1-143
Loading
/content/journal/jgv/10.1099/0022-1317-78-1-143
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error