1887

Abstract

An overall anti-hepatitis C virus (HCV) prevalence of 6.7% was found in a sero-epidemiological study carried out in the town of Conakry (Guinea Conakry, West Central Africa) on 1421 subjects who were either blood donors, pregnant women or in- and outpatients receiving treatment for conditions other than liver disease. Seven HCV isolates from a subsample of 73 sterile sera from this population were studied for genetic characterization and classification. The 5′NCR was analysed by the Line Probe Assay. This method assigned the isolates to genotype 2. Analysis of the 5′NCR sequences alone was unable to give a more accurate classification. Comparison of NS5b region sequences (nucleotides 7575–8196), from Guinea isolates and genotype 2 database sequences, showed evolutionary distances in the range 0.15–0.26. There was a high level of subtype heterogeneity among the genotype 2 Guinea HCV isolates. Four of the subtypes were possibly new.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-9-2073
1996-09-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/9/JV0770092073.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-9-2073&mimeType=html&fmt=ahah

References

  1. Bukh J., Purcell R. H., Miller R. H. 1993; At least 12 genotypes of hepatitis C virus predicted by sequence analysis of the putative El gene of isolates collected worldwide. Proceedings of the National Academy of Sciences, USA 90:8234–8238
    [Google Scholar]
  2. Choo Q.-L., Richman K. H., Han J. H., Berger K., Lee C., Dong C., Gallegos C., Coit D., Medina-Selby R., Barr P. J., Weiner A. J., Bradley D. W., Kuo G., Houghton M. 1991; Genetic organization and diversity of the hepatitis C virus. Proceedings of the National Academy of Sciences, USA 88:2451–2455
    [Google Scholar]
  3. Davidson F., Simmonds P., Ferguson J. C., Jarvis L. M., Dow B. C., Follett E. A. C., Seed C. R. G., Krusius T., Lin C., Medgyesi G. A., Kiyokawa H., Olim G., Duraisamy G., Cuypers T., Saeed A. A., Teo D., Conradie J., Kew M. C., Lin M., Nuchaprayoon C., Ndimbie O. K., Yap P. L. 1995; Survey of major genotypes and subtypes of hepatitis C virus using RFLP of sequences amplified from the 5′ noncoding region. Journal of General Virology 76:1197–1204
    [Google Scholar]
  4. Felsenstein J. 1993; PHYLIP inference package, version 3.5.. Department of Genetics; University of Washington, Seattle, Wash., USA.:
    [Google Scholar]
  5. McOmish F., Chan S.-W., Dow B. C., Gillon J., Frame W. D., Crawford R. J., Yap P. L., Follett E. A. C., Simmonds P. 1993; Detection of three types of hepatitis C virus in blood donors: investigation of type-specific differences in serological reactivity and rate of alanine aminotransferase abnormalities. Transfusion 33:7–13
    [Google Scholar]
  6. McOmish F., Yap P. L., Dow B. C., Follett E. A. C., Seed C., Keller A. J., Cobain T. J., Krusius T., Kolho E., Naukkarinen R., Lin C., Lai C., Leong S., Medgyesi G. A., Hejjas M., Kiyokawa H., Fukada K., Cuypers T., Saeed A. A., Alrasheed A. M., Lin M., Simmonds P. 1994; Geographical distribution of hepatitis C virus genotypes in blood donors - an international collaborative survey. Journal of Clinical Microbiology 32:884–892
    [Google Scholar]
  7. Mellor J., Holmes E. C., Jarvis L. M., Yap P. L., Simmonds P. The International HCV Collaborative Study Group 1995; Investigation of the pattern of hepatitis C virus sequence diversity in different geographical regions: implications for virus classification. Journal of General Virology 76:2493–2507
    [Google Scholar]
  8. Rapicetta M., Attili A. F., Mele A., De Santis A., Chionne P., Cristiano K., Spada E., Giuliani E., Carli L., Goffredo F., Capocaccia L. 1992; Prevalence of hepatitis C virus antibodies and hepatitis C virus-RNA in an urban population. Journal of Medical Virology 37:87–92
    [Google Scholar]
  9. Simmonds P., Holmes E. C., Cha T.-A., Chan S.-W., McOmish F., Irvine B., Beall E., Yap P. L., Kolberg J., Urdea M. S. 1993; Classification of hepatitis C virus into six major genotype and a series of subtypes by phylogenetic analysis of the NS-5 region. Journal of General Virology 74:2391–2399
    [Google Scholar]
  10. Stuyver L., Rossau R., Wyseur A., Duhamel M., Vanderborght B., Van Heuverswyn H., Maertens G. 1993; Typing of hepatitis C virus isolates and characterization of new subtypes using a line probe assay. Journal of General Virology 74:1093–1102
    [Google Scholar]
  11. Tanaka S. 1994; Type C hepatitis and hepatocellular carcinoma in Japan. In Viral Hepatitis and Liver Disease pp 713–715 Edited by Nishioka K., Suzuki H., Mishiro S., Oda T. Tokyo: Springer-Verlag;
    [Google Scholar]
  12. Tokita H., Okamoto H., Lizuka H., Kishimoto J., Tsuda F., Lesmana L. A., Miyakawa Y., Mayumi M. 1996; Hepatitis C virus variants from Jakarta, Indonesia classifiable into novel genotypes in the second (2e and 2f), tenth (10a) and eleventh (11a) genetic groups. Journal of General Virology 77:293–301
    [Google Scholar]
  13. Xu L.-Z., Larzul D., Delaporte E., Brechot C., Kremsdorf D. 1994; Hepatitis C virus genotype 4 is highly prevalent in central Africa (Gabon). Journal of General Virology 752393–2398
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-9-2073
Loading
/content/journal/jgv/10.1099/0022-1317-77-9-2073
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error