1887

Abstract

Sequence analysis of a 795 nucleotide region of the fish rhabdovirus viral haemorrhagic septicaemia virus (VHSV) genome revealed one complete and one partial ORF of 369 and 153 nucleotides, respectively. The latter ORF probably encodes the amino-terminal part of the L (polymerase) protein. The former ORF potentially encodes a 122 amino acid protein. The location of this ORF as well as the size and deduced structure of the translation product indicate that it represents a homologue of the non-virion (NV) protein of the related infectious haematopoietic necrosis virus (IHNV). Antisera raised against prokaryotically expressed NV protein of VHSV and IHNV were used to detect NV expression in VHSV- and IHNV-infected cells by Western Blot and immunofluorescence analyses. We present here the sequence of the VHSV NV gene and demonstrate the presence of IHNV and VHSV NV proteins in virus-infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-6-1259
1996-06-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/6/JV0770061259.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-6-1259&mimeType=html&fmt=ahah

References

  1. Benmansour A., Paubert G., Bernard J., DeKinkelin P. 1994; The polymerase-associated protein (M1) and the matrix protein (M2) from a virulent and avirulent strain of viral hemorrhagic septicemia virus (VHSV), a fish rhabdovirus. Virology 198:602–612
    [Google Scholar]
  2. Bernard J., Lecocq-Xhonneux F., Rossius M., Thiry M. E., DeKinkelin P. 1990; Cloning and sequencing the messenger RNA of the N gene of viral haemorrhagic septicaemia virus. Journal of General Virology 71:1669–1674
    [Google Scholar]
  3. Chomzynski P., Sacchi N. 1987; Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  4. DeKinkelin P., Dorson M., Hattenberger-Baudouy A. M. 1982; Interferon synthesis in trout and carp after viral infection. Developmental and Comparative Immunology Supplement 2167–174
    [Google Scholar]
  5. DeKinkelin P., LeBerre M. 1979; Mass virus production in fish cell system. Developments in Biological Standardisation 42:99–104
    [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  7. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  8. Hill B., Underwood B. O., Smale C. J., Brown F. 1975; Physico-chemical and serological characterization of five rhabdo-viruses infecting fish. Journal of General Virology 27:369–378
    [Google Scholar]
  9. Kozak M. 1989; The scanning model for translation: an update. Journal of Cell Biology 108:229–241
    [Google Scholar]
  10. Kurath G., Ahern K. G., Pearson G. D., Leong J. C. 1985; Molecular cloning of the six mRNA species of infectious hematopoietic necrosis virus, a fish rhabdovirus, and gene order determination by R-loop mapping. Journal of Virology 53:469–476
    [Google Scholar]
  11. Kurath G., Leong J. C. 1985; Characterization of infectious hematopoietic necrosis virus mRNA species demonstrates a non-virion rhabdovirus protein. Journal of Virology 53:462–468
    [Google Scholar]
  12. Kurath G., Leong J. C. 1987; Transcription in vitro of infectious haematopoietic necrosis virus, a fish rhabdovirus. Journal of General Virology 68:1767–1771
    [Google Scholar]
  13. Lenoir G., DeKinkelin P. 1975; Fish rhabdoviruses: comparative study of protein structure. Journal of Virology 16:259–262
    [Google Scholar]
  14. Mourton C., Bearzotti M., Piechaczyk M., Paolucci F., Pau B., Bastide J.-M., DeKinkelin P. 1990; Antigen-capture ELISA for viral haemorrhagic septicaemia virus serotype I. Journal of Virological Methods 29:325–334
    [Google Scholar]
  15. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. 1995 Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses Vienna & New York: Springer Verlag;
    [Google Scholar]
  16. Rose J. K. 1980; Complete intergenic and flanking sequences from the genome of vesicular stomatitis virus. Cell 19:415–421
    [Google Scholar]
  17. Schütze H., Enzmann P.-J., Kuchling R., Mundt E., Niemann H., Mettenleiter T. C. 1995; Complete genomic sequence of the fish rhabdovirus infectious haematopoietic necrosis virus. Journal of General Virology 76:2519–2527
    [Google Scholar]
  18. Thiry M., Lecocq-Xhonneux F., Dheur I., Renard A., DeKinkelin P. 1991; Sequence of a cDNA carrying the glycoprotein gene and part of the matrix protein M2 gene of viral haemorrhagic septicaemia virus, a fish rhabdovirus. Biochimica et Biophysica Acta 1090:345–347
    [Google Scholar]
  19. Tordo N., Poch O., Ermine A., Keith G., Rougeon F. 1986; Walking along the rabies genome: is the large G-L intergenic region a remnant gene?. Proceedings of the National Academy of Sciences, USA 83:3914–3918
    [Google Scholar]
  20. Wolf K. 1988 Fish viruses and viral diseases New York: Cornell University Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-6-1259
Loading
/content/journal/jgv/10.1099/0022-1317-77-6-1259
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error