1887

Abstract

Two rotavirus strains, E210 and E212, implicated in epidemics of gastroenteritis in children in central and northern Australia during 1993–1994, exhibited the unusual combination of a ‘short’ RNA electrophoretic pattern and subgroup II specificity. The outer capsid protein VP7 was found by PCR typing and sequence analysis to be related to that of serotype G2 viruses. Both strains displayed a novel pattern of reactivity to G2-specific monoclonal antibodies that correlated with sequence variation in the antigenic regions of VP7. The VP4 serotype of E210 and E212 was determined as P1B in an enzyme immunoassay, consistent with other G2 viruses. Analysis of the VP6 gene indicated significant identity (98–99%) with other human subgroup II viruses. Northern hybridization analysis of E210 RNA using total genome probes derived from the prototype strains RV4 and RV5 indicated that E210 was derived from multiple gene reassortment between rotaviruses belonging to different genetic types.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-6-1223
1996-06-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/6/JV0770061223.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-6-1223&mimeType=html&fmt=ahah

References

  1. Bishop R. F. 1994; Natural history of rotavirus infections. In Viral Infections of the Gastrointestinal Tract 2nd edn Edited by pp. 131–167 Kapikian A. Z. New York: Marcel Dekker;
    [Google Scholar]
  2. Coulson B. S. 1993; Typing of human rotavirus VP4 by an enzyme immunoassay using monoclonal antibodies. Journal of Clinical Microbiology 31:1–8
    [Google Scholar]
  3. Coulson B. S., Tursi J. M., McAdam W. J., Bishop R. F. 1986; Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. Virology 154:302–313
    [Google Scholar]
  4. Coulson B. S., Unicomb L. E., Pitson G. E., Bishop R. F. 1987; Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping of human rotaviruses. Journal of Clinical Microbiology 25:509–515
    [Google Scholar]
  5. Coulson B. S., Kirkwood C. D., Masendycz P. J., Bishop R. F., Gerna G. 1995; Amino acids involved in distinguishing between monotypes of rotavirus G serotypes 2 and 4. Journal of General Virology 77:239–245
    [Google Scholar]
  6. Dyall-Smith M. L., Holmes I. H. 1984; Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Research 12:3973–3982
    [Google Scholar]
  7. Estes M. K., Cohen J. 1989; Rotavirus genome structure and function. Microbiological Reviews 53:410–449
    [Google Scholar]
  8. Gorziglia M., Hoshino Y., Nishikawa K., Maloy W. L., Jones R. W., Kapikian A. Z., Chanock R. M. 1988; Comparative sequence analysis of the genomic segment 6 of four rotaviruses each with a different subgroup specificity. Journal of General Virology 69:1659–1669
    [Google Scholar]
  9. Gouvea V., Brantly M. 1995; Is rotavirus a population of reassortants?. Trends in Microbiology 3:159–162
    [Google Scholar]
  10. Gouvea V., Glass R. I., Woods P., Taniguchi K., Clark H. F., Forrester B., Fang Z.-Y. 1990; Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. Journal of Clinical Microbiology 28:276–282
    [Google Scholar]
  11. Green K. Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A. Z., Chanock R. M., Flores J. 1987; Comparison of the amino acid sequences of the major neutralization protein of four rotavirus serotypes. Virology 161:153–159
    [Google Scholar]
  12. Greenberg H., McAuliffe V., Valdesuso J., Wyatt R., Flores J., Kalica A., Hoshino Y., Singh N. 1983; Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infection and Immunity 39:91–99
    [Google Scholar]
  13. Kaga E., Nakagomi O. 1994; Recurrent circulation of single nonstructural gene substitution reassortants among human rotaviruses with a short RNA pattern. Archives of Virology 136:63–71
    [Google Scholar]
  14. Kobayashi N., Taniguchi K., Urasawa T., Urasawa S. 1994; Effect of the selection pressure with anti-VP7 and anti-VP4 neutralizing monoclonal antibodies on reassortment formation between two human rotaviruses. Archives of Virology 135:383–396
    [Google Scholar]
  15. Krishnan T., Burke B., Naik T. N., Desselberger U. 1994; Molecular epidemiology of human rotaviruses in Manipur: genome analysis of rotaviruses of long electropherotype and subgroup I. Archives of Virology 134:279–292
    [Google Scholar]
  16. Lopez S., Espinoza R., Greenberg H. B., Arias C. F. 1994; Mapping the subgroup epitopes of rotavirus protein VP6. Virology 204:153–162
    [Google Scholar]
  17. Nakagomi O., Nakagomi T. 1991; Molecular evidence for naturally occurring single VP7 gene substitution reassortant between human rotaviruses belonging to two different genogroups. Archives of Virology 119:67–81
    [Google Scholar]
  18. Nakagomi O., Nakagomi T. 1993; Interspecies transmission of rotaviruses studied from the perspective of genogroup. Microbiology and Immunology 37:337–348
    [Google Scholar]
  19. Palombo E. A., Bishop R. F. 1994; Sequences of VP6 genes of human rotavirus strain RV3 and its vaccine derivative. Journal of General Virology 75:2415–2419
    [Google Scholar]
  20. Palombo E. A., Bishop R. F. 1995; Genetic and antigenic characterization of a serotype G6 human rotavirus isolated in Melbourne, Australia. Journal of Medical Virology 47:348–354
    [Google Scholar]
  21. Steele A. D., Alexander J. J. 1988; The relative frequency of subgroup I and II rotaviruses in black infants in South Africa. Journal of Medical Virology 24:321–327
    [Google Scholar]
  22. Urasawa S., Urasawa T., Taniguchi K. 1986; Genetic reassortment between two human rotaviruses having different serotype and subgroup specificities. Journal of General Virology 67:1551–1559
    [Google Scholar]
  23. Ward R. L., Nakagomi O., Knowlton D. R., McNeal M. M., Nakagomi T., Clemens J. D., Sack D. A., Schiff G. M. 1990; Evidence for natural reassortants of human rotaviruses belonging to different genogroups. Journal of Virology 64:3219–3225
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-6-1223
Loading
/content/journal/jgv/10.1099/0022-1317-77-6-1223
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error