1887

Abstract

Using a series of new insertion/expression vectors, we constructed a set of recombinant vaccinia viruses (recVV) encoding the murine T cell costimulatory molecules mB7-1 or mB7-2, or both together in the same construct. On infection with replication incompetent and non-cytopathic recVV, several tumour cell lines expressed the respective molecules and bound to CTLA-4. The highest binding capacity was found when both mB7 molecules were co-expressed. Mouse B16.F10 melanoma cells expressing mB7-1 or mB7-2 provided effective costimulation for proliferation of resting CD4 T cells in the presence of concanavalin A and plate-bound anti-T cell receptor antibodies, respectively. If mB7-1 and mB7-2 were delivered together on the same cell, the proliferative response of CD4 T cells increased further. The costimulatory effect could be blocked with CTLA-4, the soluble ligand for B7 molecules. The possibility of engineering tumour cells using recVV holds implications for the future design of vaccination strategies.

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1996-12-01
2021-10-16
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References

  1. Chen C., Nabavi N. 1994; In vivo induction of T cell anergy by blocking B7 and early T cell costimulatory molecule ETC-1/B7-2. Immunity 1:147–154
    [Google Scholar]
  2. Davison A. J., Moss B. 1989; Structure of vaccinia vims early promoters. Journal of Molecular Biology 210:749–769
    [Google Scholar]
  3. Earl P. L., Moss B. 1995; Expression of proteins in mammalian cells using vaccinia. In Current Protocols in Molecular Biology 1st edn, pp 16.15.1–16.18.10 Edited by Ausubel F. M., Brent R., Kingston R. E. New York: Greene Publishing Association;
    [Google Scholar]
  4. Falkner F. G., Moss B. 1990; Transient dominant selection of recombinant vaccinia vims. Journal of Virology 64:3108–3111
    [Google Scholar]
  5. Fearon E. R., Pardoll D. M., Itaya T., Golumbek P. T., Levitsky H. I., Simons J. W., Karasuyana H., Vogelstein B., Frost P. 1990; Interleukin-2 production by tumor cells bypasses T helper function in the generation of an antitumor response. Cell 60:397–403
    [Google Scholar]
  6. Freeman G. J., Freedman A. S., Segil J. M., Lee G., Whitman J. F., Nadler L. M. 1989; B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells. Journal of Immunology 143:2714–2722
    [Google Scholar]
  7. Freeman G. J., Gribben J. G., Boussiotis V. A., Ng J. W., Restivo V. A., Lombard L. A., Gray G. S., Nadler L. M. 1993; Cloning of B7-2: a CTLA-4 counter-receptor that costimulates human T cell proliferation. Science 262:909–911
    [Google Scholar]
  8. Gimmi C. D., Greeman G. J., Gribben J. G., Sugita K., Freedman A. S., Morimoto C., Nadler L. M. 1981; B-cell surface antigen B7 provides a costimulatory signal that induces T cells to proliferate and secrete interleukin 2. Proceedings of the National Academy of Sciences, USA 88:6575–6579
    [Google Scholar]
  9. Goebel S. J., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete DNA sequence of vaccinia virus. Virology 179:247–266
    [Google Scholar]
  10. Golumbek P. T., Lazenby A. J., Levitsky H. I., Jaffee L. M., Kara-suyama H., Baker M., Pardoll D. M. 1991; Treatment of established renal cancer by tumor cells engineered to secrete interleukin-4. Science 254:713–716
    [Google Scholar]
  11. Harding F. A., McArthur J. G., Gross J. A., Raulet D. H., Allison J. P. 1992; CD28 mediated signalling co-stimulates murine T cells and prevents induction of anergy in T cell clones. Nature 356:607–609
    [Google Scholar]
  12. Linsley P. S., Brady W., Grosmaire L., Aruffo A., Damle N. K., Ledbetter J. A. 1991; Binding of the B cell activation antigen B7 to CD28 costimulates T cell proliferation and interleukin 2 mRNA accumulation. Journal of Experimental Medicine 173:721–730
    [Google Scholar]
  13. Linsley P. S., Greene J. L., Brady W., Bajorath J., Ledbetter J. A., Peach R. 1994; Human B7-1 (CD80) and B7-2 (CD86) bind with similar avidities but distinct kinetics to CD28 and CTLA-4 receptors. Immunity 1:793–801
    [Google Scholar]
  14. Liu Y., Janeway C. A. 1992; Cells that present both specific ligand and costimulatory activity are most efficient inducers of clonal expansion of normal CD4 T cells. Proceedings of the National Academy of Sciences, USA 89:3845–3849
    [Google Scholar]
  15. Topatian S. L., Rivoltini L., Mancini M., Markus N. R., Robbins P. F., Kawakami Y., Rosenberg S. A. 1994; Human CD4+ T cells specifically recognize a shared melanoma-associated antigen encoded by the tyrosinase gene. Proceedings of the National Academy of Sciences, USA 91:9461–9465
    [Google Scholar]
  16. Tsung K., Yim J. H., Marti W. R., Buller M. L., Norton J. A. 1996; Gene expression and cytopathic effect of chemically inactivated vaccinia virus. Journal of Virology 70:165–171
    [Google Scholar]
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